Exploring Anti-Klebsiella Pneumoniae Activity of Probiotic Lactobacilli of Curd Origin

Mandal, Sandip; Halder, Debashis

doi:10.31080/asmi.2018.01.0038

Cited by 5 publications

(8 citation statements)

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“…MSU3IR strain, which against pathogenic bacteria: Staphylococcus aureus and Pseudomonas aeruginosa, displayed higher bacteriocin activity (393.2 ± 2.61 to 556.0 ± 5.34 AU/ml) in MRS medium, compared to the activity value (341.2 ± 2.36 to 473.2 ± 3.96 AU/ml) as recorded in Lactobacillus selection broth. Earlier, the 'R' values of curd lactobacilli strains ranged 3.00 -13.17 mm, while the bacteriocin activity, in terms of arbitrary units (AU/ml), ranged 155.60 -293.33, against MDR Klebsiella pneumoniae clinical isolates (Mandal and Halder, 2018).…”

Section: Resultsmentioning

confidence: 99%

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Insights into the antagonism of Lactobacillus fermentum curd isolate against Gram-positive and Gram-negative pathogenic bacteria

Halder¹,

Mandal²

2018

Biosci. Biotech. Res. Comm

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This communication characterizes the curd isolate of lactic acid bacteria having the capacity to antagonize human pathogenic bacteria. The commercially available curd, in sealed form in a plastic cup, was procured from Malda town market (West Bengal state, India) and processed microbiologically, using de Man Rogosa Sharpe medium, for the isolation of lactic acid bacteria. The pure bacteria culture obtained was identifi ed, by phenotypic characterization through conventional methods, as Lactobacillus fermentum, and designated as LMEM 22. The Lactobacillus fermentum LMEM 22 curd isolate had mixed antibiotic susceptibility patterns, showing resistance (ZDI: ≤15 mm) to amikacin, ciprofl oxacin, kanamycin, methicillin and vancomycin, sensitivity (ZDI: ≥ 21 mm) to ampicillin, amoxyclav, gentamycin, cefotaxime, imipenem, meropenem and tetracycline, and intermediate susceptibility (ZDI: 16-20 mm) to cfoxitin and trimethoprim. The L. fermentum LMEM22 antagonizes both gram-negative: Acinetobacter baumannii, Pseudomonas aeruginosa, Escherichia coli, Proteus vulgaris, Klebsiella pneumoniae, Salmonella enterica serovar Typhi, and gram-positive: Staphylococcus aureus, Bacillus cereus, Enterococcus faecalis, Listeria monocytogenes, bacterial pathogens, following agar overlay (ZDI: 17±1.73 mm to 20±1.00 mm, for gram-positive, and 18±2.00 mm to 33±2.65 mm for gram-negative bacteria) as well as agar-well diffusion (ZDI: 10.67±2.08 mm to 12±1.00 mm, for gram-positive, and 13.00±2.65 mm to 18.00±3.00 mm, for gram-negative bacteria) techniques. The overall bacteriocin activity (AU/ml) of Lactobacillus fermentum LMEM 22 for the test bacterial pathogens ranged 142.27-240.00, and the 'R' value ranged 5.5-13.5. This study underlines the usefulness of locally available lactic acid bacteria in designing the probiotic microorganisms for biotherapy.

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Section: Resultsmentioning

confidence: 99%

“…following agar-well diffusion (Tagg, 1971;Halder et al, 2017) and agar overlay (Shokryazdan et al, 2018) methods, as described and interpreted earlier (Shokryazdan et al, 2014, Halder et al, 2017, Mandal and Halder, 2018.…”

Section: Methodsmentioning

confidence: 99%

Insights into the antagonism of Lactobacillus fermentum curd isolate against Gram-positive and Gram-negative pathogenic bacteria

Halder¹,

Mandal²

2018

Biosci. Biotech. Res. Comm

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“…According to study conducted by Sharma et al there was a significant increase in resistant K. pneumoniae from 7.5% in 2018 to 21.4% in 2022, while XDR K. pneumoniae among mechanically ventilated ICU patients significantly increased from 62.5% in 2018 to 71% in 2022. 25 Multi-drug-resistant K. pneumoniae has become an urgent risk to public health 9 . Thus, in the era of antimicrobial resistance, the possible use of probiotics as antibacterial agents is an emerging issue with a rapidly expanding field of applications.…”

Section: Discussionmentioning

confidence: 99%

“…36 This finding is in contrast to the result obtained by Halder and Mandal who got greater zone of inhibition values for L. rhamnosus than L. acidophilus against K. pneumoniae isolates. 9 Such differences in the antibacterial activity of the Lactobacilli could be due to the strain and their source variation, in the later study the strains were obtained from curd (L. acidophilus LMEM8 and L. rhamnosus LMEM9) while in our study we used standard strains of L. acidophilus and L. rhamnosus (L. acidophilus MTCC 10307 and L. rhamnosus MTCC 1408). In this study, we also compared the inhibitory action of treated and untreated suspension of L. acidophilus and L. rhamnosus and found out that untreated suspension possess greater inhibitory effect in both cases.…”

Section: Discussionmentioning

confidence: 99%

“…The need of such new therapies is important in the treatment of health careassociated infections caused by antibiotic resistant (MDR: multidrug resistant; XDR: extensively drugresistant; PDR: pan-drug resistant) bacteria. 9 The lactobacilli have extensively been studied for their remarkable capacity to inhibit the growth of other microorganisms through various mechanisms. 10,11 As drug resistant strains of K. pneumoniae are increasing at an alarming rate, antimicrobial susceptibility pattern was also determined to identify the resistant strains and to investigate whether probiotics have any action on the same.…”

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Evaluation of antimicrobial activity of Lactobacillus acidophilus and Lactobacillus rhamnosus against clinically isolated Klebsiella pneumoniae: an in vitro study

Anil,

Raj,

M. M.

et al. 2024

Int J Res Med Sci

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Background: Klebsiella pneumoniae are Gram-negative opportunistic pathogen, belonging to the family Enterobacteriaceae that can cause severe nosocomial infections particularly in immuno-compromised individuals. They exhibit co-resistance to multiple antibiotics which emphasize the need for non-antibiotic therapies. The goal of the presented study was to investigate the antimicrobial ability of probiotic Lactobacilli on clinical isolates of K. pneumoniae. Methods: In this cross-sectional study, antimicrobial activities of probiotic L. acidophilus and L. rhamnosus on K. pneumoniae were evaluated by Agar overlay interference technique. Clear zone around Lactobacilli were taken as positive inhibition. Antibiotic susceptibility profiles of K. pneumoniae were determined by Kirby-Bauer disk diffusion method, analyzed using interpretive standards of CLSI M100-S33 and categorized into MDR, XDR and Non MDR groups. Statistical analysis was done using descriptive statistics such as mean and standard error and inferential statistics such as ANOVA single factor. Results: K. pneumoniae exhibited positive inhibition with both the probiotic strain. On comparing the zone of inhibition of L. acidophilus and L. rhamnosus (both treated-pH adjusted and untreated), L. acidophilus had greater zone of inhibition against K. pneumoniae but concluded that statistically the values are insignificant (p>0.05). Based on antibiotic susceptibility pattern of K. pneumoniae, 63% of isolates were XDR, 3% were MDR and 34% were Non MDR Conclusions: It can be concluded that L. acidophilus and L. rhamnosus had significant inhibitory effect against K. pneumoniae in vitro and should be further studied for their human health benefit.

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Lactiplantibacillus plantarum isolates from natural honey (Malda, India): Probiotic potentiality and antibacterial activity analysis by in vitro methods

Roy,

Mandal

2024

Food and Humanity

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Exploring Anti-Klebsiella Pneumoniae Activity of Probiotic Lactobacilli of Curd Origin

Abstract: Keywords: Probiotic Lactobacilli; MDR Klebsiella pneumoniae; Antagonistic Activity; Bacteriocin Activity; MAR Index Four curd lactobacilli probiotic strains: Lactobacillus animalisnosus LMEM9, were utilized in the current study. The methodol-

Cited by 5 publications

References 11 publications

Insights into the antagonism of Lactobacillus fermentum curd isolate against Gram-positive and Gram-negative pathogenic bacteria

Insights into the antagonism of Lactobacillus fermentum curd isolate against Gram-positive and Gram-negative pathogenic bacteria

Evaluation of antimicrobial activity of Lactobacillus acidophilus and Lactobacillus rhamnosus against clinically isolated Klebsiella pneumoniae: an in vitro study

Lactiplantibacillus plantarum isolates from natural honey (Malda, India): Probiotic potentiality and antibacterial activity analysis by in vitro methods

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