Optimization of Analytical Procedure for In-hospital Rapid Quantification of Serum Level of Favipiravir in the Pharmacological Treatment of COVID-19

Moriiwa, Yukiko; Morikawa, Go; Okazawa, Katsuko; Yanagida, Akio

doi:10.2116/analsci.21n004

Cited by 11 publications

(12 citation statements)

References 17 publications

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“…This method consists of an initial pretreatment step using solid-phase extraction (SPE) and a subsequent separation and detection step using high-performance liquid chromatography (HPLC). Interestingly, our previous study showed that FPV selectively formed complexes with ferric (Fe 3+ ) and cupric (Cu 2+ ) ions [12]. Based on this finding, we speculated that blood FPV levels may be sensitive to differences in serum ferritin levels considering that ferritin complexes can store Fe 3+ ions.…”

Section: Introductionmentioning

confidence: 88%

“…The detailed method for quantifying blood (serum) FPV levels was reported previously (The terms "blood" FPV level and "serum" FPV level are interchangeable in this study) [12]. Briefly, a centrifugal spin-cartridge (MonoSpinC 18 , GL Science, Inc., Tokyo, Japan) was used for the SPE treatment prior to HPLC analysis of FPV.…”

Section: Measurement Of Serum Fpv Levelsmentioning

confidence: 99%

“…result demonstrating that FPV is selectively complexed with Fe 3+ ions in an aqueous buffer (pH 7.4) solution [12], we hypothesized that complexation occurs between stored iron-bound ferritin and FPV in the blood of COVID-19 patients with hyperferritinemia. We also hypothesized that elevated blood FPV levels (after FPV administration) were inversely associated with blood ferritin levels.…”

Section: Figmentioning

confidence: 99%

“…In the present study, the practical FPV quantification method we developed previously [12] was well applied and easily performed by the clinical staff of our hospital, representing little medical or economic burden. In the near future, we expect that this method will be utilized more widely for therapeutic drug monitoring of blood FPV levels and would be clinically useful in assessing COVID-19 treatment with FPV.…”

Section: Figmentioning

confidence: 99%

“…To monitor blood FPV levels, we previously developed a rapid, inhospital (i.e., in our facility) method for quantifying blood FPV levels in COVID-19 patients [12]. This method consists of an initial pretreatment step using solid-phase extraction (SPE) and a subsequent separation and detection step using high-performance liquid chromatography (HPLC).…”

Section: Introductionmentioning

confidence: 99%

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Elevated blood favipiravir levels are inversely associated with ferritin levels and induce the elevation of uric acid levels in COVID-19 treatment: A retrospective single-center study

Morikawa

Kubota

Kondo

et al. 2022

Journal of Infection and Chemotherapy

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Introduction Measurement of blood Favipiravir (FPV) levels and accumulation of data in COVID-19 patients are critical for assessing FPV efficacy and safety. We performed a retrospective study based on measurements of blood levels of FPV and related factors in COVID-19 patients admitted to our hospital. Furthermore, we also investigated the association between blood FPV levels and uric acid level alterations before and after FPV administration. Methods We enrolled 27 COVID-19 patients who had received FPV treatment at Hokushin General Hospital from April 1 to December 31, 2020. Age, gender, COVID-19 severity, presence of comorbidities, and laboratory data for each subject were investigated to identify factors that correlate with blood FPV levels. Uric acid levels were measured before and after FPV administration and a difference between the levels ( i.e. , a change of uric acid level) was evaluated. Results When a significant univariate variable was input by the stepwise method and a combination of variables that maintained statistical superiority was searched, serum ferritin was the only factor that independently affected blood FPV level. Furthermore, in the high-FPV group (20 μg/mL or more), a significant increase in uric acid levels was observed after FPV administration. The increment value was significantly larger than that in the low-FPV group (less than 20 μg/mL). Conclusions Ferritin level was an important independent factor inversely affecting blood FPV level. Furthermore, a high blood FPV level induced the elevation of uric acid levels in COVID-19 treatment.

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Section: Introductionmentioning

confidence: 88%

Section: Measurement Of Serum Fpv Levelsmentioning

confidence: 99%

Section: Figmentioning

confidence: 99%

Section: Figmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Elevated blood favipiravir levels are inversely associated with ferritin levels and induce the elevation of uric acid levels in COVID-19 treatment: A retrospective single-center study

Morikawa

Kubota

Kondo

et al. 2022

Journal of Infection and Chemotherapy

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Core–shell nanocomposite of flower-like molybdenum disulfide nanospheres and molecularly imprinted polymers for electrochemical detection of anti COVID-19 drug favipiravir in biological samples

Wang

Xin

et al. 2022

Microchim Acta

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A novel electrochemical sensor is reported for the detection of the antiviral drug favipiravir based on the core–shell nanocomposite of flower-like molybdenum disulfide (MoS 2 ) nanospheres and molecularly imprinted polymers (MIPs). The MoS 2 @MIP core–shell nanocomposite was prepared via the electrodeposition of a MIP layer on the MoS 2 modified electrode, using o-phenylenediamine as the monomer and favipiravir as the template. The selective binding of target favipiravir at the MoS 2 @MIP core–shell nanocomposite produced a redox signal in a concentration dependent manner, which was used for the quantitative analysis. The preparation process of the MoS 2 @MIP core–shell nanocomposite was optimized. Under the optimal conditions, the sensor exhibited a wide linear response range of 0.01 ~ 100 nM (1.57*10 −6 ~ 1.57*10 −2 μg mL −1 ) and a low detection limit of 0.002 nM (3.14*10 −7 μg mL −1 ). Application of the sensor was demonstrated by detecting favipiravir in a minimum amount of 10 μL biological samples (urine and plasma). Satisfied results in the recovery tests indicated a high potential of favipiravir monitoring in infectious COVID-19 samples. Graphical abstract Supplementary Information The online version contains supplementary material available at 10.1007/s00604-022-05213-9.

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Functional metal–organic framework as high-performance adsorbent for selective enrichment of pharmaceutical contaminants in aqueous samples

Zhao

Xie

et al. 2022

Chemical Engineering Journal

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Optimization of Analytical Procedure for In-hospital Rapid Quantification of Serum Level of Favipiravir in the Pharmacological Treatment of COVID-19

Cited by 11 publications

References 17 publications

Elevated blood favipiravir levels are inversely associated with ferritin levels and induce the elevation of uric acid levels in COVID-19 treatment: A retrospective single-center study

Elevated blood favipiravir levels are inversely associated with ferritin levels and induce the elevation of uric acid levels in COVID-19 treatment: A retrospective single-center study

Core–shell nanocomposite of flower-like molybdenum disulfide nanospheres and molecularly imprinted polymers for electrochemical detection of anti COVID-19 drug favipiravir in biological samples

Functional metal–organic framework as high-performance adsorbent for selective enrichment of pharmaceutical contaminants in aqueous samples

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