Optimization of an ultrasound-assisted extraction method for the determination of parabens and bisphenol homologues in human saliva by liquid chromatography-tandem mass spectrometry

Moscoso-Ruiz, Inmaculada; Gálvez-Ontiveros, Yolanda; Cantarero-Malagón, Samuel; Rivas, Ana; Zafra, Alberto

doi:10.1016/j.microc.2021.107122

Cited by 20 publications

(22 citation statements)

References 25 publications

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“…The main derivatization method used to adapt analytes that are excessively polar and have low volatility to GC determinations is silylation with trimethylsilyl reagent (TMS), N , O -bis(trimethylsilyl)trifluoroacetamide (BSTFA) or other reagents. This type of reaction requires anhydrous conditions and a multistage procedure [ 27 ]. This method of derivatization is not recommended for compounds from the BP group due to the risk of overestimating the obtained results [ 28 ].…”

Section: Introductionmentioning

confidence: 99%

USAEME-GC/MS Method for Easy and Sensitive Determination of Nine Bisphenol Analogues in Water and Wastewater

et al. 2022

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A new, simple and sensitive method for isolating nine compounds from the bisphenol group (analogues: A, B, C, E, F, G, Cl2, Z, AP) based on one-step liquid–liquid microextraction with in situ acylation followed by gas chromatography-mass spectrometry was developed and validated using influent and effluent wastewaters. The chemometric approach based on the Taguchi method was used to optimize the main conditions of simultaneous extraction and derivatization. The recoveries of the proposed procedure ranged from 85 to 122%, and the repeatability expressed by the coefficient of variation did not exceed 8%. The method’s limits of detection were in the range of 0.4–64 ng/L, and the method’s limits of quantification ranged from 1.3 to 194 ng/L. The developed method was used to determine the presence of the tested compounds in wastewater from a municipal wastewater treatment plant located in northeastern Poland. From this sample, eight analytes were detected. Concentrations of bisphenol A of 400 ng/L in influent and 100 ng/L in effluent were recorded, whereas other bisphenols reached 67 and 50 ng/L for influent and effluent, respectively. The removal efficiency of bisphenol analogues in the tested wastewater treatment plant ranged from 7 to approximately 88%.

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Section: Introductionmentioning

confidence: 99%

USAEME-GC/MS Method for Easy and Sensitive Determination of Nine Bisphenol Analogues in Water and Wastewater

et al. 2022

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“…The fellow helped to design the experiments and to apply the extraction procedure. Final results about the optimisation and validation have been published recently (Moscoso‐Ruiz et al, 2022 ). Briefly, 1 g of fresh saliva is subjected to a protein precipitation with ACN and acidic medium, followed by an ultrasound‐assisted extraction with ethanol and re‐extracted with acetone (30 min, 35% power).…”

Section: Description Of Work Programmementioning

confidence: 99%

“…This is considered of particular concern as this population can develop diabetes mellitus type II and cardiovascular disease among others. Recently different scientific studies have started to consider additional factors playing a role in the onset of obesity including diet and lifestyle habits, genetics (Herbert, 2008;Heindel and Blumberg, 2019), living environment (Nappi et al, 2016) and exposure to obesogens. Obesogens can be found in food, dust, water, drugs and personal care products (PCPs), so they can enter the human body by oral and dermal contact or inhalation (Monneret, 2017).…”

Section: Introductionmentioning

confidence: 99%

Association between dietary exposure to bisphenols and body mass index in Spanish schoolchildren

Stecca

Moscoso-Ruiz

Gálvez-Ontiveros

et al. 2022

EFS2

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The increase in children obesity worldwide has been of particular concern in recent decades. Environmental factors have been proposed as contributors to obesity, and there is a growing concern over obesogens, environmental chemicals with potential obesity‐related endocrine‐disrupting properties. In this regard, bisphenol A (BPA) and its analogues are suspected to have obesogenic properties. Current document report on the activities of the fellow, undertaken during the fourth, 2020–2021 cycle of the EU‐FORA programme at the University of Granada, Institute of Nutrition and Food Science, in Spain. The work programme offered by the hosting site was related to the extrapolation of bisphenols exposure following the determination of these compounds in food frequently consumed by children and in their biological samples. The fellow has participated in the recruitment of the study population in the health centres. In addition, she has participated in the collection of the children biological samples, anthropometric measurements and dietary surveys and in the optimisation of the laboratory methodology for the extraction of bisphenols in biological samples. All these activities also provided the fellow an opportunity to develop her data science related skills, which will benefit her professional development. In addition, the fellow gained an overview of various topics related to food safety risk assessment by attending the EU‐FORA dedicated training modules.

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“…The detection limit of their method was 0.23 to 2.54 ng/ml. Moscoso-Ruiz et al [22] detected parabens and bisphenols in human saliva by using ultrasound-assisted extraction for purification and UPLC-MS/MS for detection. Liu et al [23] used SPE + HPLC-MS/MS to study the presence of 24 steroids in aquatic environments and organisms.…”

Section: Introductionmentioning

confidence: 99%

“…UPLC-MS/MS has become one of the main popular analytical methods for EDC detection [22,25], but due to the varied properties of EDCs, multiclass and high-throughput detection methods in biological samples still need to be developed. In our group, the analysis methods for 31 EDCs (including seven androgens, five glucocorticoids, six progesterones, seven estrogens, and five industrial compounds) in water [31,32] and fish samples [25] were established by mixed cation exchange (MCX) cartridgebased SPE combined with HPLC-MS/MS.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of 31 endocrine disrupting chemicals in fish plasma by solid‐phase extraction coupled with ultra‐performance liquid chromatography‐tandem mass spectrometry

Zhou

Bao

Huang

et al. 2022

J of Separation Science

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Solid phase extraction combined with ultra‐performance liquid chromatography‐tandem mass spectrometry was developed for the simultaneous determination of 31 endocrine‐disrupting chemicals in fish plasma. The strong anion exchange/primary‐secondary amine cartridge and the mixed cation exchange cartridge were used in tandem instead of using a single mixed cation exchange cartridge for sample purification. Suitable eluents were selected for each of the two cartridges: 4.5% ammonia/acetonitrile solution for cartridges in tandem and acetone:n‐hexane (V:V = 3:7) for the strong anion exchange/primary‐secondary amine cartridge alone. With this optimized Solid phase extraction method, the recoveries of 31 endocrine disrupting chemicals were between 43.0% and 131.3%, the method detection limits were 0.45 to 1.35 ng/ml, and the limits of quantitation were 1.50–4.50 ng/ml. The innovative pretreatment method that connects two cartridges in tandem is well positioned to mitigate the matrix effects of fish plasma, thereby improving the accuracy of multiclass endocrine‐disrupting chemicals determination. The significance of this method is to facilitate the application of the fish plasma model for the environmental risk assessment of endocrine‐disrupting chemicals.

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Optimization of an ultrasound-assisted extraction method for the determination of parabens and bisphenol homologues in human saliva by liquid chromatography-tandem mass spectrometry

Cited by 20 publications

References 25 publications

USAEME-GC/MS Method for Easy and Sensitive Determination of Nine Bisphenol Analogues in Water and Wastewater

USAEME-GC/MS Method for Easy and Sensitive Determination of Nine Bisphenol Analogues in Water and Wastewater

Association between dietary exposure to bisphenols and body mass index in Spanish schoolchildren

Simultaneous determination of 31 endocrine disrupting chemicals in fish plasma by solid‐phase extraction coupled with ultra‐performance liquid chromatography‐tandem mass spectrometry

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