Optimization of an amplified system for the detection of Clostridium tyrobutyricum on nitrocellulose filters by use of monoclonal antibody in a gelified medium

Nedellec, M.; Cleret, J. J.; Robreau, Georges; Talbot, F.; Malcoste, R.

doi:10.1111/j.1365-2672.1992.tb05184.x

Cited by 6 publications

(7 citation statements)

References 14 publications

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“…Apart from not exclusively detecting C. tyrobutyricum, MPN methods for enumeration of butyric acid bacteria spores have several other disadvantages: the analysis time is long (4 to 7 days) and the results have a high uncertainty (which is inherent to most MPN procedures). Alternative methods for detection of C. tyrobutyricum are available, for instance methods based on qPCR (Herman et al 1995, Lopez-Enriquez et al 2007 or immunological techniques (Nedellec et al 1992, Lavilla et al 2010. However, these methods are currently not used for routine analyses in the dairy sector, presumably because they are relatively laborious and costly.…”

Section: Clostridium Speciesmentioning

confidence: 99%

Silage and the safety and quality of dairy foods: a review

Driehuis

2013

AFSci

113

115

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Silage contains a number of potential hazards to the safety and quality of milk and dairy products. This paper reviews the present knowledge about silage as a source of (1) spores of anaerobic spore-formers (Clostridium species) and aerobic spore-formers (mainly Bacillus and Paenibacillus species), (2) the zoonotic pathogenic bacteria Listeria monocytogenes and Escherichia coli, and (3) mycotoxins. A distinction is made between field-derived mycotoxins, i.e. mycotoxins that are formed during growth of crops in the field, and ensilage-derived mycotoxins, i.e. mycotoxins that are formed after ensiling. The routes of transmission of these hazards from feed to milk, the effect of pasteurization of milk, and reduction strategies are discussed. Aerobic deterioration of silages is a major factor influencing levels of spores of both aerobic and anaerobic spore-formers, L. monocytogenes, and certain mycotoxins.

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Section: Clostridium Speciesmentioning

confidence: 99%

Silage and the safety and quality of dairy foods: a review

Driehuis

2013

AFSci

113

115

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“…Immunoassays have also been developed for the vegetative cells of both sporeformers and nonsporeformers (Hartman et al 1992). An immunoassay specific for the detection of spores of Clostridium tyrobutyricum was developed because of the defects caused in cheese fermentations due to the presence of even very small numbers of spores of this butyric anaerobe during fermentation (Nedellec et al 1992). To date, however, there are no commercially available ELISAs for the rapid detection of total spore loads in food products or ingredients, or for detection of spores of a particular physiological category, i.e.…”

Section: Introductionmentioning

confidence: 99%

MONOCLONAL ANTIBODY‐BASED ELISAS FOR THE DETECTION OF BACTERIAL SPORES ¹

Quinlan

Foegeding

1998

Rapid Methods Automation Mic

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Monoclonal antibodies against bacterial spores were used to develop ELISAs to detect a range of spores. ELISAs utilizing anti‐Bacillus spore antibodies detected spores of Bacillus megaterium ATCC 12872, Bacillus stearothermophilus ATCC 7953, Bacillus subtilis var. globigii and Bacillus cereus T. They did not detect Bacillus subtilis A, Bacillus coagulans ATCC 56177 or Bacillus licheniformis ATCC 9789. One ELISA utilizing anti‐Clostridium spore antibodies detected spores of Desulfotomaculum nigrificans ATCC 7946, Clostridium perfringens ATCC 3624 and B. stearothermophilus ATCC 7953, but not spores of Clostridum sporogenes PA3679 or Clostridium botulinum 62A. Whole milk, skim milk, gelatin and starch all interfered to some degree with the abilities of the assays to detect spores. Neither centrifugation nor immunomagnetic bead separation provided sufficient concentration of spores from the food matrix to be applicable to the ELISAs developed. These results demonstrate the ability to detect a range of spores using monoclonal antibody‐based ELISAs. Such assays may, however, be limited by sensitivity and interference from food components.

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“…An alternative method is based on membrane filtration of the milk followed by growing of the bacteria on the membrane (1). The identity of C. tyrobutyricum may be confirmed by immunological methods (12) or by a DNA hybridization method (13).…”

mentioning

confidence: 99%

A direct PCR detection method for Clostridium tyrobutyricum spores in up to 100 milliliters of raw milk

Herman¹,

Block²,

Waes³

1995

Appl Environ Microbiol

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A direct detection method for Clostridium tyrobutyricum spores in up to 100 ml of raw milk is presented. The bacterial spores are concentrated by centrifugation after chemical extraction of the milk components. The vegetative cells are selectively lysed, and their DNA is digested and washed away. Afterwards, the DNA is liberated from the spores by microwave treatment. For the identification of the C. tyrobutyricum DNA, a two-step PCR method with two nested pairs of primers is used. The primers were derived from the 16S-23S rRNA spacer region of C. tyrobutyricum, and the specificity of each of them for C. tyrobutyricum is demonstrated. The detection limit can be estimated to be between 3 and 30 spores in 100 ml of raw milk.

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Optimization of an amplified system for the detection of Clostridium tyrobutyricum on nitrocellulose filters by use of monoclonal antibody in a gelified medium

Cited by 6 publications

References 14 publications

Silage and the safety and quality of dairy foods: a review

Silage and the safety and quality of dairy foods: a review

MONOCLONAL ANTIBODY‐BASED ELISAS FOR THE DETECTION OF BACTERIAL SPORES ¹

A direct PCR detection method for Clostridium tyrobutyricum spores in up to 100 milliliters of raw milk

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Optimization of an amplified system for the detection of Clostridium tyrobutyricum on nitrocellulose filters by use of monoclonal antibody in a gelified medium

Cited by 6 publications

References 14 publications

Silage and the safety and quality of dairy foods: a review

Silage and the safety and quality of dairy foods: a review

MONOCLONAL ANTIBODY‐BASED ELISAS FOR THE DETECTION OF BACTERIAL SPORES 1

A direct PCR detection method for Clostridium tyrobutyricum spores in up to 100 milliliters of raw milk

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MONOCLONAL ANTIBODY‐BASED ELISAS FOR THE DETECTION OF BACTERIAL SPORES ¹