Optimization of amylase production from Bacillus sp. using statistics&lt;br&gt;based experimental design

Zambare, Vasudeo

doi:10.9755/ejfa.v23i1.5311

Cited by 36 publications

(20 citation statements)

References 30 publications

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“…The optimum levels of the said variables were then determined by employing RSM. Present findings are also in accordance with the findings of Zambare, [18] in terms of the independent variables obtained. There are a number of reports in literature which suggests that lower temperatures enhance the production of EPS [19,7].…”

Section: C Validation Of the Modelsupporting

confidence: 92%

Optimization of Various Process Parameters Using Response Surface Methodology for Exopolysaccharide Production from a Novel Strain Pediococcus acidilactici KM0 (Accession Number KX671557) Isolated from Milk Cream

Sharma¹,

Sharma²,

Bajwa³

et al. 2017

IJERMT

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Abstractediococcus acidilactici KM0 (accession number KX671557) isolated from milk cream was optimized by response surface methodology by using the Design Of Experiments (DOE) to evaluate the interactive effects of the process parameters i.e. incubation time (h), temperature (•C), pH, carbon concentration, nitrogen concentration production for the production of EPS . Maximum EPS production of 32.64 mg/ml observed after 24 h incubation at 35 •C using 1.50 % and 3% of carbon and nitrogen concentration respectively, at pH 6.5.

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Section: C Validation Of the Modelsupporting

confidence: 92%

Optimization of Various Process Parameters Using Response Surface Methodology for Exopolysaccharide Production from a Novel Strain Pediococcus acidilactici KM0 (Accession Number KX671557) Isolated from Milk Cream

Sharma¹,

Sharma²,

Bajwa³

et al. 2017

IJERMT

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“…In this study inoculum size of 4% gave maximum enzyme production. Zambare () reported inoculum size of 2.95% optimized through central composite design of response surface methodology for amylase production by Bacillus sp.…”

Section: Resultsmentioning

confidence: 99%

Potato peels: A potential food waste for amylase production

Mushtaq

Irfan

Tabssum

et al. 2016

J Food Process Engineering

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In the present study, thermophilic and amylase producing bacterium Bacillus subtilis K‐18 (KX881940) was isolated from local environment and identified by 16S rRNA gene sequencing technology. The amylase producing potential was checked by starch hydrolysis test using potato peels as carbon source. Central composite design (CCD) of response surface methodology (RSM) was used to optimize multiple process parameters such as substrate concentration, inoculum size, yeast extract, and pH. The optimized conditions obtained from RSM model were substrate concentration (2%), inoculum size (4%), yeast extract concentration (0.6%), and pH (5) yielding amylase activity of 1394.145 IU/ml after 24 hr of fermentation period at 50 °C. The maximum saccharification by indigenously produced amylase was 32.03% after 24 hr of incubation. The results confirmed that amylase produced by this newly isolated Bacillus subtilis K‐18 (KX881940) could be potent strain for industrial process particular for biofuel production. Practical application The enzyme produced in this study could be effectively used in hydrolysis of starch to produce sugars which could be widely used for the production of various chemicals such ethanol and other products through fermentation. This enzyme also has potential application in textile industry.

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“…has been carried out by Ramkumar et al (2011). Also Zambare (2011) with the help of response surface methodology, optimized amylase enzyme production from Bacillus sp. by using cellulosic material.…”

Section: Effects Of Different Cellulosic Substrates On the Productionmentioning

confidence: 99%

Production and characterization of cellulolytic enzymes by isolated<br>Klebsiella sp. PRW-1 using agricultural waste biomass

Waghmare

Kshirsagar

Saratale

et al. 2014

Emir. J. Food Agric

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The efficient cellulolytic microorganism was isolated from soil samples collected from Shivaji University campus. Biochemical test and phylogenetic analysis of isolated culture identified as Klebsiella sp. PRW-1. The isolated culture could utilize pure cellulosic substrates (carboxymethylcellulose (CMC) and avicel) and different agricultural wastes like sugarcane bagasse, sugarcane barbojo, sorghum husks, grass powder, corn straw and paddy straw by producing a large amount of endoglucanase, exoglucanase, β-glucosidase, filter paperase (FPU), xylanase and glucoamylase. The reducing sugar production was found higher in the presence of grass powder and sugarcane barbojo. Effects of different physic-chemical parameters to achieve maximum cellulolytic enzymes production were systematically investigated. The effects of supplementation of different metals additives as well as the stability in the presence of higher temperature, pH and commercial detergents, on cellulolytic enzymes were also studied. The foregoing result increases the applicability of the strain for the utilization and bioconversion of lignocellulosic biomass that could be used for bioenergy production.

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Optimization of amylase production from Bacillus sp. using statistics<br>based experimental design

Cited by 36 publications

References 30 publications

Optimization of Various Process Parameters Using Response Surface Methodology for Exopolysaccharide Production from a Novel Strain Pediococcus acidilactici KM0 (Accession Number KX671557) Isolated from Milk Cream

Optimization of Various Process Parameters Using Response Surface Methodology for Exopolysaccharide Production from a Novel Strain Pediococcus acidilactici KM0 (Accession Number KX671557) Isolated from Milk Cream

Potato peels: A potential food waste for amylase production

Production and characterization of cellulolytic enzymes by isolated<br>Klebsiella sp. PRW-1 using agricultural waste biomass

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