Optimization of Agricultural Waste Substrate as an Alternative Medium for Xylan in Producing Xylanase Enzymes by Thermophilic Bacteria

Irdawati, I; Sofiyyana, A; Advinda, Linda; Fiffendy, M; Salvia, S; Syamsuardi, S; Agustien, Anthoni; Rilda, Yetria; Yahya, Yasin

doi:10.1088/1742-6596/1940/1/012052

Cited by 3 publications

(2 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An essential component in the manufacture of xylanase enzymes is the choice of substrate type and the make-up of the appropriate medium. According to the study's findings, rice straw was the best substrate for the growth of the SSA 2 isolate as a replacement for pure xylan [14]. Jacobsen et al stated that the hemicellulose content in rice straw was 24.5% [15].…”

Section: The Specific Activity Of the Xylanase Enzymementioning

confidence: 99%

Specific Thermophilic Bacterial Xylanase Enzyme Activity Using Rice Straw as Substrate and Its Possibility as an Eco-friendly Fabric Bleach

Irdawati,

Hidayati,

Advinda

et al. 2023

Advances in Biological Sciences Research

View full text Add to dashboard Cite

Extracellular enzyme xylanase has a wide range of industrial uses. The xylanase enzymes made by thermophilic bacteria can hydrolyze xylan into xylose and xylooligosaccharides. Rice straw, for example, is a natural carbon source that can be utilized to create xylan carbon sources. One of xylanase's uses in industry is as an environmentally friendly bleaching agent (biobleaching). This study's objectives were to ascertain the xylanase enzyme's specific activity at varied substrate concentrations and to evaluate the enzyme's potential impact on fabric brightness. This investigation is experimental. This is an experimental study using RAL that includes three replications and six treatments. The xylanase enzyme's contribution to the fabric's whitening will be evaluated using the kappa number. At a substrate concentration of, the xylanase enzyme's specific activity peaked 4%, or 0.419 U/mg protein, based on the results. The kappa number results show that the xylanase enzyme from agricultural waste substrates can reduce the kappa number (2.21) whereas the kappa number without xylanase is higher. This is supported by the potential activity of the xylanase enzyme on fabric brightness (3.42).

show abstract

Section: The Specific Activity Of the Xylanase Enzymementioning

confidence: 99%

Specific Thermophilic Bacterial Xylanase Enzyme Activity Using Rice Straw as Substrate and Its Possibility as an Eco-friendly Fabric Bleach

Irdawati,

Hidayati,

Advinda

et al. 2023

Advances in Biological Sciences Research

View full text Add to dashboard Cite

show abstract

“…Industrially, xylanases are considered eco-friendly where they are consumed in the processing of xylan for different applications such as in pharmaceutical industries (including the preparation of xylooligosaccharides (XOS), an oligomer produced as a result of the catalytic action of xylanase on xylan ) 23 , the paper and pulp industry (for improving the e ciency of bleaching without consumption of harsh chemicals as chlorine) 24,25 , food industry 26 and generation of biofuel 27 . Hence, our goal is to purify and elucidate the enzymatic characteristics of an extracellular xylanase from a novel marine bacterium and study its biotechnological applications.…”

Section: Introductionmentioning

confidence: 99%

Xylanolytic Biotechnological Applications of Extracellular Xylanase Isolated from Marine Bacillus Subtilis AKM1

Mahmoud

Elhakim

Abdelhamid

et al. 2023

Preprint

View full text Add to dashboard Cite

Xylanase enzyme from marine bacteria was investigated for production improvement and studying its effect on agriculture wastes for use in biotechnological applications. By identification of the selective strain by using 16SRNA, the isolate was a new strain called Bacillus Subtilis AKM1 and has the accession number MK377251. The optimum culture conditions for xylanase production were as follows: incubation period (48 hrs.), initial pH (7.0), agitation speed (100 rpm), and incubation temperature(40°C). After purification, xylanase showed maximal activity at pH 7.0 and 50 °C. Metal ions such as Mn2+, Ca2+, and Fe2+ increased the residual activity by increasing the concentration, while Ag+, SDS, and EDTA completely inhibited the activity at higher concentrations. the molecular weight was 34 KDa, Km 0.044 mM, and Vmax 22.222. Xylooligosaccharides produced by enzymatic hydrolysis using the purified xylanase showed antimicrobial activity, antioxidant activity, and anti-tumor activity against breast and colon cancer cells.

show abstract

Bacillus spp. enzymatic activity to support circular economy

Dujkovic,

Pajcin,

Vlajkov

et al. 2023

ACTA PERIOD TECHN

View full text Add to dashboard Cite

Agricultural and industrial waste represents the most abundant renewable resource in the biosphere and is readily available in substantial quantities. Recently, there has been a growing focus on harnessing the potential of these types of waste as raw materials for producing value-added products, such as microbial enzymes, using solid-state or submerged fermentation systems. Among all the bacterial genera utilized in the production of industrially important enzymes, the Bacillus genus stands out as the most extensively employed for this purpose. In this study, the production of various enzymes such as protease, pectinase, cellulase, xylanase, lipase, and gelatinase was evaluated using the different Bacillus strains isolated from vegetables? rhizosphere. The growth zones and halos were observed to determine production of the following enzymes: cellulases, proteases, pectinases, xylanases, gelatinases and lipases, confirming the breakdown of complex substrates in the medium. Bacillus Pap 3 exhibited the highest EAI (enzymatic activity index) value (4.00) for pectinase activity, indicating its remarkable extracellular level of pectinase performance. The highest enzymatic activity in terms of halo zone diameters was achieved in the case of cellulases and xylanases production by the isolates Mah 1a and Mah 1b. Further research would be directed towards the production of the tested enzymes by Bacillus spp. on the waste streams of food and agroindustry, but also towards the utilization of the proven enzymatic activity to obtain other microbial value-added products to develop viable bioprocess solutions usable in the circular economy context.

show abstract

Optimization of Agricultural Waste Substrate as an Alternative Medium for Xylan in Producing Xylanase Enzymes by Thermophilic Bacteria

Cited by 3 publications

References 9 publications

Specific Thermophilic Bacterial Xylanase Enzyme Activity Using Rice Straw as Substrate and Its Possibility as an Eco-friendly Fabric Bleach

Specific Thermophilic Bacterial Xylanase Enzyme Activity Using Rice Straw as Substrate and Its Possibility as an Eco-friendly Fabric Bleach

Xylanolytic Biotechnological Applications of Extracellular Xylanase Isolated from Marine Bacillus Subtilis AKM1

Bacillus spp. enzymatic activity to support circular economy

Contact Info

Product

Resources

About