Specific Thermophilic Bacterial Xylanase Enzyme Activity Using Rice Straw as Substrate and Its Possibility as an Eco-friendly Fabric Bleach

Abstract

Extracellular enzyme xylanase has a wide range of industrial uses. The xylanase enzymes made by thermophilic bacteria can hydrolyze xylan into xylose and xylooligosaccharides. Rice straw, for example, is a natural carbon source that can be utilized to create xylan carbon sources. One of xylanase's uses in industry is as an environmentally friendly bleaching agent (biobleaching). This study's objectives were to ascertain the xylanase enzyme's specific activity at varied substrate concentrations and to evaluate the enzyme's potential impact on fabric brightness. This investigation is experimental. This is an experimental study using RAL that includes three replications and six treatments. The xylanase enzyme's contribution to the fabric's whitening will be evaluated using the kappa number. At a substrate concentration of, the xylanase enzyme's specific activity peaked 4%, or 0.419 U/mg protein, based on the results. The kappa number results show that the xylanase enzyme from agricultural waste substrates can reduce the kappa number (2.21) whereas the kappa number without xylanase is higher. This is supported by the potential activity of the xylanase enzyme on fabric brightness (3.42).