Optimization of Acetone-Fractionation for 1-Palmitoyl-2-Oleoyl-3-Oleoyl Glycerol and 1-Palmitoyl-2-Oleoyl-3-Palmitoyl Glycerol by Response Surface Methodology

Abstract

1-Palmitoyl-2-oleoyl-3-oleoyl glycerol (POO) and 1-palmitoyl-2-oleoyl-3-palmitoyl glycerol (POP) were enriched from palm stearin using an acetone fractionation process. Response surface methodology was employed to optimize the purity of POO (Y1, %) and POP (Y2, %) along with POO＋POP content (Y3, g) based on independent variables such as fractionation temperature (X1, 25, 30, and 35 o C) and the ratio of palm stearin to acetone (X2, 1:3, 1:6 and 1:9, w/v). Fractionation conditions were optimized to maximize Y1, Y2, and Y3, in which fractionation temperature was 29.3 o C with a 1:5.7 acetone ratio. With such parameters, 60.9% of POP and 23.8% of POO purity were expected with a 75% yield (3.0 g) of POO and POP.