Optimization of a quantitative HPLC determination of potentially anticarcinogenic flavonoids in vegetables and fruits

Hertog, M.G.L.; Hollman, P.C.H.; Venema, D.P.

doi:10.1021/jf00021a023

Cited by 649 publications

(475 citation statements)

References 13 publications

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“…They have the ability to inhibit lipid peroxidation and lipoxygenase in vitro. Consumption of flavonoid containing fruits and vegetables has been linked to protection against cancer and heart disease (Hertog et al, 1992;Atoui et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Contents of constituents and antioxidant activity of seed and pulp extracts of Annona coriacea and Annona sylvatica

et al. 2015

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The antioxidant potential of fruit pulp and seeds of extracts of the Annona coriacea, and A. sylvatica (Annonaceae) were investigated, as well contents total phenolics, flavonoids, condensed tannins and ascorbic acid. Was used to determine the antioxidant activity the 1,1-diphenyl-1-picrylhydrazyl free radical (DPPH), β-carotene bleaching and ABTS radical cation method. The total phenol, total flavonoid, condensed tannin, and ascorbic acid contents were measured spectrophotometrically. In this study, the pulp and seeds of the fruits were extracted using methanol/water (8:2) for maceration. The seed extracts of A. coriacea demonstrated a moderate antioxidant effect with free radical scavenging activity of 31.53%, by the DPPH test, 51.59% by the β-carotene bleaching test and 159.50 µM trolx/g of extract in the ABTS assay. We found that the hydromethanolic seed extract of A. coriacea had high total phenol (147.08 ± 4.20 mg of GAE/g of extract) and flavonoid (131.18 ± 2.31 mg of QE/g of extract) content. This indicated that the antioxidant activity of the extracts was related to the contents of these constituents.Keywords: Annona, antioxidant activity, phytochemistry. Conteúdo

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Section: Discussionmentioning

confidence: 99%

Contents of constituents and antioxidant activity of seed and pulp extracts of Annona coriacea and Annona sylvatica

et al. 2015

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“…The nutrient contents of the diets, including hesperetin, naringenin and quercetin, were analysed from the duplicate portions at the Agricultural Research Center of Finland (Jokioinen, Finland). Flavonoids were extracted and analysed according to the method of Hertog et al (1992) with a minor modification (a gradient was used in high-performance liquid chromatography). The limit of quantitation for the analysis of the flavonoids in question was 2 mg=100 g of fresh weight of diet.…”

Section: Dietary Analysesmentioning

confidence: 99%

Plasma concentrations of the flavonoids hesperetin, naringenin and quercetin in human subjects following their habitual diets, and diets high or low in fruit and vegetables

et al. 2002

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Objectives: To determine the fasting plasma concentrations of quercetin, hesperetin and naringenin in human subjects consuming their habitual diets, and diets either high or low in fruit and vegetables. To investigate whether plasma concentrations of flavanones can serve as biomarkers of their intake. Design: This was a cross-over, strictly controlled dietary intervention consisting of a 2 week baseline period, and two 5 week dietary periods with a 3 week wash-out period in between. The low-vegetable diet contained few fruit and vegetables and no citrus fruit. The high-vegetable diet provided various fruits and vegetables daily including on average one glass of orange juice, one-half orange and one-half mandarin. Subjects: Thirty-seven healthy females. Results: The high-vegetable diet provided 132 mg of hesperetin and 29 mg of naringenin. The low-vegetable diet contained no flavanones. The mean plasma hesperetin concentration increased from 12.2 nmol=l after the low-vegetable diet to 325 nmol=l after the high-vegetable diet. The respective increase for naringenin was from < 73.5 nmol=l for all subjects to a mean value of 112.9 nmol=l. The mean plasma quercetin concentration was 52 nmol=l after the baseline period, during which habitual diets were consumed, and it did not change significantly during the intervention. Interindividual variation in the plasma levels of hesperetin and naringenin was marked and, after the baseline and wash-out periods, and the low-vegetable diet, a majority of the samples had plasma flavanone levels below the limit of detection. After the high-vegetable diet, hesperetin and naringenin were detectable in 54 and 22% of all samples. Quercetin was detectable in nearly all samples after all study periods. Conclusion: Hesperetin, naringenin and quercetin are bioavailable from the diet, but the plasma concentrations of hesperetin and naringenin are poor biomarkers of intake.

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“…Vitamin E was extracted according to Grimm and Tiews (1972) and determined chromatographically (McMurray and Blanch¯ower, 1979). Selenium was determined according to Welz and Melcher (1984) and avonoids according to Hertog et al (1992). Total lipids were isolated according to Osborne and Voogt (1978).…”

Section: Examinationsmentioning

confidence: 99%

Fruit and fish consumption: a possible explanation for population differences in COPD mortality (The Seven Countries Study)

Tabak

Feskens²,

Heederik

et al. 1998

Eur J Clin Nutr

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Objective: To investigate whether average intake of antioxidants, fruits, vegetables and ®sh may help to explain international differences in chronic obstructive pulmonary disease (COPD) mortality. Design: Ecological analysis using information on baseline diet and the 25-year COPD mortality rate in the 16 cohorts of the Seven Countries Study. Setting: Population-based cohorts. Subjects: Men aged 40 ± 59 years at baseline. Methods: Dietary information was collected at baseline in small random samples of each cohort. In 1987 the reported foods were bought locally and analysed chemically. After 25 years of follow-up the underlying cause of death of those who died was established centrally. COPD mortality rate ratios were calculated, for a change equivalent to 10% of the overall mean consumption of a dietary factor. Results: We observed independent inverse associations between 25-year COPD mortality and baseline consumption of fruits (rate ratio 0.49; 95% con®dence interval 0.36 ± 0.67) and ®sh (rate ratio, 0.97; 95% con®dence interval 0.93 ± 1.00), after adjustment for potential confounders. COPD mortality showed no statistically signi®cant association with intake of antioxidants or vegetables. Fruit and ®sh consumption together explained about 67% of the variance in the COPD mortality rates of the cohorts. Conclusions: Fruit and ®sh consumption may partly explain population differences in COPD mortality. This is in accordance with suggestions for a relationship between fruit and ®sh consumption and COPD observed in studies in individuals.

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Optimization of a quantitative HPLC determination of potentially anticarcinogenic flavonoids in vegetables and fruits

Cited by 649 publications

References 13 publications

Contents of constituents and antioxidant activity of seed and pulp extracts of Annona coriacea and Annona sylvatica

Contents of constituents and antioxidant activity of seed and pulp extracts of Annona coriacea and Annona sylvatica

Plasma concentrations of the flavonoids hesperetin, naringenin and quercetin in human subjects following their habitual diets, and diets high or low in fruit and vegetables

Fruit and fish consumption: a possible explanation for population differences in COPD mortality (The Seven Countries Study)

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