Contents of constituents and antioxidant activity of seed and pulp extracts of Annona coriacea and Annona sylvatica

Benites, RS; Formagio, Anelise Samara Nazari; Argandoña, EJ; Volobuff, CR; Trevizan, LN; Vieira, M.C.; Silva, S. Ravi P.

doi:10.1590/1519-6984.21313

Cited by 40 publications

(33 citation statements)

References 45 publications

(39 reference statements)

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“…DPPH is a stable free radical interacting with antioxidant substances, which transfer electrons or atoms of hydrogen to DPPH, neutralizing ("scavenging") the free radical. This process can be observed as a change in the color of the reaction agent from violet to yellow and a reduction in the absorbance at 517 nm (Benites et al, 2015;Banerjee et al, 2005). The data showed that the EtOH and AcOEt extracts exhibited excellent free radical scavenging activity (IC 50 < 100 µg/mL).…”

Section: Resultsmentioning

confidence: 98%

Assessment of the antibacterial, cytotoxic and antioxidant activities of Morus nigra L. (Moraceae)

et al. 2017

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This study was carried out to assess the antibacterial, cytotoxic and antioxidant activities of extracts of Morus nigra L. HPLC was used to determine the fingerprint chromatogram of the crude ethanolic extract (Mn-EtOH). The antibacterial effect was assessed through the method of microdilution. The cytotoxicity was tested against human tumour cell lines using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. The total phenolic and flavonoid contents were also assessed through the Folin-Ciocalteu and aluminum chloride methods, respectively. Antioxidant activities of the extracts were evaluated by using 2,2-diphenyl-1-picrylhydrazil (DPPH) radical scavenging and β-carotene-linoleic acid bleaching methods. The presence of phenolic compounds in Mn-EtOH was confirmed using HPLC. The extracts showed activity against most microorganisms tested. The extracts did not show any expressive antiproliferative effect in the assessment of cytotoxicity. The most significant total phenolic content was 153.00 ± 11.34 mg of gallic acid equivalent/g to the ethyl acetate extract (AcOEt). The total flavonoid content was 292.50 ± 70.34 mg of catechin equivalent/g to the AcOEt extract, which presented the best antioxidant activity (IC 50 50.40 ± 1.16 μg/mL) for DPPH scavenging. We can conclude that this species shows strong antibacterial and antioxidant activities, as well as weak cytotoxic effects.Keywords: antibacterial, antioxidant, cytotoxic, Moraceae, Morus nigra. Avaliação das atividades antibacteriana, citotóxica e antioxidante de Morus nigra L. (Moraceae) ResumoEste estudo foi realizado para avaliar as atividades antibacteriana, citotóxica e antioxidante de extratos de Morus nigra L. HPLC foi utilizado para determinar o perfil de compostos fenólicos do extrato etanólico bruto (Mn-EtOH). O efeito antibacteriano foi avaliado através do método de microdiluição. A citotoxicidade foi testada contra linhagens celulares de tumores humanos utilizando o ensaio do brometo de 3-(4,5-dimetil-2-tiazolil)-2,5-difenil-2H-tetrazólio (MTT). O conteúdo total de compostos fenólicos e flavonoides também foi avaliado por meio dos métodos de Folin-Ciocalteu e cloreto de alumínio, respectivamente. A atividade antioxidante dos extratos foi avaliada por meio do sequestro do radical livre 2,2-difenil-1-picrilhidrazil (DPPH) e co-oxidação do sistema β-caroteno-ácido linoleico. A presença de compostos fenólicos em Mn-EtOH foi confirmada utilizando HPLC. Os extratos mostraram atividade contra a maioria dos microrganismos testados. Os extratos não mostraram qualquer efeito antiproliferativo expressivo na avaliação da citotoxicidade. O conteúdo fenólico total mais significativo foi de 153,00 ± 11,34 mg de equivalente de ácido gálico/g para o extrato acetato de etila (AcOEt). O conteúdo de flavonoides totais foi de 292,50 ± 70,34 mg de equivalente de catequina/g para o extrato AcOEt, que apresentou a melhor atividade antioxidante (IC 50 50,40 ± 1,16 mg/mL) para o sequestro do DPPH. Podemos concluir que esta espécie apres...

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Section: Resultsmentioning

confidence: 98%

Assessment of the antibacterial, cytotoxic and antioxidant activities of Morus nigra L. (Moraceae)

et al. 2017

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“…Analisis meliputi kadar hara N, P, dan K daun pada awal generatif (30 HSP) dan kadar hara N, P, dan K buah menggunakan metode Kjeldahl untuk N, metode pengabuan basah untuk P dan K. Analisis kadar total fenol (Benites et al, 2015) dengan pengekstrak etanol. Sampel buah untuk analisis kadar hara N, P, dan K buah dan analisis kadar total fenol menggunakan buah yang sudah dikeringkan dalam April 2019 oven pada suhu 60 o C selama tiga hari sampai kadar air konstan.…”

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Hasil dan Kualitas Okra (Abelmoschus esculentus L. Moench.) Merah dan Okra Hijau dengan Jenis Pupuk yang Berbeda

Manik¹,

Melati

Kurniawati

et al. 2019

J Agron Indonesia

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Okra fruit contains phenolic compounds as natural antioxidant. The aim of this research was to analyze the effect of different types of fertilizers on production and quality of two okra varieties. The experiment was conducted in October 2017 to February 2018 at Bogor Agricultural University experimental field at Leuwikopo, Darmaga, Bogor (-6033’49.3”SL, 106043’30.7’’EL). The experiment used nested randomized complete block design with 2 factors and 4 replications. The first factor consisted of two okra varieties with different colour (red and green okra). The second factor was fertilizer types consisted of no fertilizer, organic (cow manure), combination of organic + inorganic, and inorganic fertilizer. The results showed that fertilizer types significantly affected plant height and fruit N content but did not affect fruit production. Red okra had a higher plant height, lower number of leaves and leaf K content than those of the green okra. The interaction effect of both factors was significant on total phenolic content in fruit and P content in leaf. The highest total phenolic content in fruit was showed by red okra with inorganic fertilizer, although it was not significantly different to those of red okra with combination organic + inorganic fertilizer, and also to green okra with organic fertilizer. The highest P content in leaf was found in red okra without fertilizer, however it was not significantly different to red okra with combination organic + inorganic fertilizer, green okra with organic fertilizer, and green okra with inorganic fertilizer.Keywords: inorganic fertilizer, leaf nutrient, organic fertilizer, phenolic compound

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“…Analisis kadar klorofil total, karotenoid dan antosianin menggunakan metode yang diadopsi dari Sims dan Gamon (2002), dilakukan di Laboratorium Pascapanen di Departemen Agronomi dan Hortikultura IPB. Analisis fenolik total dengan menggunakan metode yang diadopsi dari Benites et al (2015) dilaksanakan di Laboratorium Departemen Ilmu dan Teknologi Pangan.…”

Section: Bahan Dan Metodeunclassified

Keragaman Kualitas Buah pada Dua Varietas Okra (Abelmoschus esculentus L. Moench) dari Umur Panen Berbeda

Aplugi¹,

Melati

Kurniawati

et al. 2019

J Agron Indonesia

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Okra (Abelmoschus esculentus L. Moench) is a functional vegetables that has high economic value and it is usedas health purposes due to high bioactive compounds and dietary fiber contents. Okra pod is gradually harvested followsflowering time. The purpose of this study was to evaluate the effect of harvest age on Nitrogen (N), Phosphorus (P), Potassium(K), pigment and phenolic contents of two okra varities. The experiment was conducted from October 2017 to February 2018at IPB experimental station in Leuwikopo, Dramaga, Bogor. The experimental field is located at ± 185 m asl with 6033’49.3”S and 106043’30.7” E. The experiment used single factor, namely harvest time, in randomized complete block nested in factorof okra variety with 4 replicates. The varieties of okra were Zahira (red okra) and Naila (green okra), while harvest agesconsisted of 5, 7, 9, and 11 days after the anthesis (DAA). The results of the study showed significant effect of harvest age onN, P, anthocyanin and phenolic contents. Okra pod harvested at 5 DAA, had the highest N, P and anthocyanin levels; whilethe highest phenolic level was obtained from okra pod harvested at 7 DAA. There were interactions effect between harvesttime and okra varieties on chlorophyll and carotenoid contents. The red okra harvested at 5 DAA had the highest content oftotal chlorophyll (536.83 μg g-1) and carotenoid (147.79 μg g-1).

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Contents of constituents and antioxidant activity of seed and pulp extracts of Annona coriacea and Annona sylvatica

Cited by 40 publications

References 45 publications

Assessment of the antibacterial, cytotoxic and antioxidant activities of Morus nigra L. (Moraceae)

Assessment of the antibacterial, cytotoxic and antioxidant activities of Morus nigra L. (Moraceae)

Hasil dan Kualitas Okra (Abelmoschus esculentus L. Moench.) Merah dan Okra Hijau dengan Jenis Pupuk yang Berbeda

Keragaman Kualitas Buah pada Dua Varietas Okra (Abelmoschus esculentus L. Moench) dari Umur Panen Berbeda

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