Optimization of a plant regeneration and genetic transformation protocol for Eucalyptusclonal genotypes

Deepika, Raj; Veale, Adri; Ma, Cathleen; Strauss, Steven H.; Myburg, Alexander Andrew

doi:10.1186/1753-6561-5-s7-p132

Cited by 6 publications

(3 citation statements)

References 3 publications

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“…Developing a regeneration protocol for this species would be an important step towards the improvement of its properties for the utilization in pulp and paper industries. Several reports regarding transformation and micropropagation methods in various eucalyptus genotypes are available (Kawaoka et al, 2006;Deepika et al, 2011;Ahad et al, 2014). E.…”

Section: Discussionmentioning

confidence: 99%

“…Micropropagation of E. camaldulensis has been documented previously in vitro via direct organogenesis (Yashoda et al, 1997;Kawaoka et al, 2006;Pinto et al, 2013) and indirect organogenesis (Mullins et al, 1997;Ho et al, 1998;Harcourt et al, 2000;Rahim et al, 2003;Dibax et al, 2005;Dibax et al, 2010;Ahad et al, 2014) and indirect somatic embryogenesis (Prakash and Gurumurthi, 2010). Deepika et al (2011) established in vitro shoot regeneration on MS basal medium supplemented by plant growth hormones (BAP, NAA and TDZ) for various eucalyptus genotypes i.e. E. grandis, E. grandis × E. nitens, E. grandis × E. camaldulensis and E. grandis × E. urophylla.…”

Section: Introductionmentioning

confidence: 98%

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DEVELOPMENT OF EFFICIENT MICROPROPAGATION SYSTEM FOR E. camaldulensis WITH RESPECT TO AGE OF EXPLANTS

Ahad¹

2018

PAKJAS

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Efficient regeneration protocol is important for mass propagation, genetic transformation and germplasm alteration of desired plants. Thus, development of an efficient protocol is of the considerable importance for further genetic manipulation. In vitro plant regeneration was studied from two age groups of seedlings (2-11 weeks and 12-21 weeks old) by using their cotyledons, hypocotyls & leaf segments as explants. Shoot organogenesis was evaluated by using different explants on MS medium supplemented with varying concentrations of phtyohormones (1mg/l BAP ; 0.2 mg/l BAP + 1mg/l Zn ; 0.5mg/l BAP + 0.1mg/l NAA; 1.5mg/l BAP + 0.5mg/l NAA). Similarly, rooting of regenerated shoots was optimized on different concentrations of IBA (0.1mg/l;0.5mg/l;1mg/l). Hypocotyls of two to eleven weeks old seedlings as explants showed the best results of direct organogenesis on BAP (0.5mg/l) and NAA (0.1mg/l) in 21 days. However, BAP (0.2 mg/l) and Zn (1mg/l) showed no growth. Indole-3-butyric acid (1 mg/l) induced rooting within a minimum period of 12 days. Overall, rate of shoot and root formation was found to be 50%, respectively. Successfully, the platelets were acclimatized in net house and further transferred to open field conditions.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

DEVELOPMENT OF EFFICIENT MICROPROPAGATION SYSTEM FOR E. camaldulensis WITH RESPECT TO AGE OF EXPLANTS

Ahad¹

2018

PAKJAS

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“…Although the primary benefit of grey water reuse is that it conserves water resources for potable uses, despite it reduces the service demand on the potable water supply (source, treatment, and transmission), demands on the downstream wastewater infrastructure (collection, treatment, and disposal), and potential impacts on the receiving environment [6]. Grey water from single house premises may also be considered to be a potential resource and may be reused on-site for garden and lawn watering, IJASR|VOL 03|ISSUE 05|2017 www.ssjournals.com toilet flushing, and laundry use depending on the type of grey water and its level of treatment.…”

Section: Introductionmentioning

confidence: 99%

Study on the efficiency of continuous flow-based constructed wetland system for grey water treatment

Devi

N.D.Shrinithivihahshini

Viji

2017

Int J of Adv in Sci Res

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Water is inevitable for our life. Due to the population growth, there is a tremendous pressure on the existing fresh water resources such as surface water and ground water. Increasing water demand and improper usage of potable water lead to scarcity of fresh water resources. Globally, treating grey water is a real constraint to minimize the problem of water scarcity. The continuous flow-based constructed wetland system for grey water treatment is a technique for reusing the domestic grey water and it is a low-cost method. The current study was aimed to evolve a suitable user-friendly treatment system for handling the household grey water. In the present study, grey water has been collected from the Bharathidasan University and it has been treated with biofiltration and rhizhodegradation techniques using continuous flow-based constructed wetland system. The system has been found as more effective for treating the Physico-chemical parameters such as suspended solids, pH, electrical conductivity, TS, TDS, DO, BOD, COD, TOC, CO 3, HCO 3, SO 4 , NO 3 , PO 4 , Ca, Mg, Na, K, total hardness, calcium hardness, chloride, and total alkalinity. The results reported the reduction in the biological oxygen demand (89%), chemical oxygen demand (81%), DO (95%), carbonate (100%), sodium (65%), and potassium (85%).It also examined the benefits and risks associated with the results in the reuse of domestic grey water for the purpose of vegetable gardening, irrigation, and toilet flushing. Consequently, this biofiltration method is natural, simple, and low cost-effective treatment in a holistic manner.

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Effects of an inducible aiiA gene on disease resistance in Eucalyptus urophylla × Eucalyptus grandis

Ouyang

2016

Transgenic Res

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N-acyl-homoserine lactones (AHLs) are metabolites of mostly gram-negative bacteria and are critical signaling molecules in bacterial quorum-sensing systems. At threshold concentrations, AHLs can activate the expression of pathogenic genes and induce diseases. Therefore, reducing AHL concentrations is a key point of disease control in plants. AHL-lactonase, which is expressed by aiiA, is widespread in Bacillus sp and can hydrolyze AHLs. In the present study, we cloned aiiA from Bacillus subtilis by PCR. A plant expression vector of aiiA was constructed and name Pcam-PPP3-aiiA, in which expression of aiiA was controlled by the pathogen-inducible plant promoter PPP3. The recombinant plasmid was transferred into Eucalyptus × urophylla × E. grandis by an Agrobacterium-mediated transformation. PCR and Southern blotting showed that aiiA was successfully integrated into the E. urophylla × E. grandis genome and its expression was induced by Ralstonia solanacearum 12 h after inoculation, as shown by reverse transcription-PCR. The transcription efficacy of aiiA increased 43.88-, 30.65-, and 18.95-fold after inoculation with R. solanacearum, Erwinia carotovora ssp. zeae (Sabet) and Cylindrocladium quinqueseptatum, respectively as shown by RT-real-time PCR. Transgenic E.urophylla × E.grandis expressing the AIIA protein exhibited significantly enhanced disease resistance compared to non-transgenic plants by delaying the onset of wilting and reducing the disease index.

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Optimization of a plant regeneration and genetic transformation protocol for Eucalyptusclonal genotypes

Cited by 6 publications

References 3 publications

DEVELOPMENT OF EFFICIENT MICROPROPAGATION SYSTEM FOR E. camaldulensis WITH RESPECT TO AGE OF EXPLANTS

DEVELOPMENT OF EFFICIENT MICROPROPAGATION SYSTEM FOR E. camaldulensis WITH RESPECT TO AGE OF EXPLANTS

Study on the efficiency of continuous flow-based constructed wetland system for grey water treatment

Effects of an inducible aiiA gene on disease resistance in Eucalyptus urophylla × Eucalyptus grandis

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