Optimization of a Method for Preparation and Repopulation of the Tracheal Matrix for Allogenic Transplantation

Kiselevsky, M. V.; Anisimova, Natalia; Olga, Lebedinskaya; Полоцкий, Б. Е.; Давыдов, М. И.

doi:10.1007/s10517-011-1270-7

Cited by 6 publications

(5 citation statements)

References 13 publications

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“…Although such biological matrices have important theoretical advantages, the decellularization process is often very harsh, and biomechanical and biochemical deterioration is often noted (Haykal et al ., ; Sun et al ., ). Other workers continue to establish the optimal decellularization protocols (Jungebluth et al ., ; Kiselevsky et al ., ; Zang et al ., ; Bijonowski et al ., ; Baiguera et al ., ; Weymann et al ., ). However, tissue‐engineering approaches that rely on cadaveric tissue are limited by the shortage of donors, potential infection and suitability of available tissue.…”

Section: Discussionmentioning

confidence: 99%

Towards reconstruction of epithelialized cartilages from autologous adipose tissue-derived stem cells

New

Ibrahim

Guasti

et al. 2016

J Tissue Eng Regen Med

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Deformities of the upper airways, including those of the nose and throat, are typically corrected by reconstructive surgery. The use of autologous somatic stem cells for repair of defects could improve quality and outcomes of such operations. The present study explored the ability of paediatric adipose-derived stem cells (pADSCs), a readily available source of autologous stem cells, to generate a cartilage construct with a functional epithelium. Paediatric ADSCs seeded on the biodegradable nanocomposite polymer, polyhedral oligomeric silsesquioxane poly(ϵ-caprolactone-urea) urethane (POSS-PCL), proliferated and differentiated towards mesenchymal lineages. The ADSCs infiltrated three-dimensional POSS-PCL nanoscaffold and chondroid matrix was observed throughout chondrogenically induced samples. In ovo chorioallantoic membrane-grafted ADSC-nanoscaffold composites were enwrapped by host vessels indicating good compatibility in an in vivo system. Furthermore, pADSCs could be induced to transdifferentiate towards barrier-forming epithelial-like cells. By combining differentiation protocols, it was possible to generate epithelial cell lined chondrogenic micromasses from the same pADSC line. This proof-of-concept study appears to be the first to demonstrate that individual pADSC lines can differentiate towards two different germ lines and be successfully co-cultured. This has important implications for bioengineering of paediatric airways and further confirms the plastic nature of ADSCs. Copyright © 2016 John Wiley & Sons, Ltd.

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Section: Discussionmentioning

confidence: 99%

Towards reconstruction of epithelialized cartilages from autologous adipose tissue-derived stem cells

New

Ibrahim

Guasti

et al. 2016

J Tissue Eng Regen Med

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“…Decellularisation involves removing cells expressing major histocompatibility complex (MHC) class I and II antigens to stop an immunogenic response using detergent. 26 Decellularisation of tissue can be done using chemical treatment of NaClO 4 ; 37 however, this method has not been widely used. Detergent-enzymatic method has been very popular and generally yields good results.…”

Section: Types Of Scaffoldsmentioning

confidence: 99%

Tubular organ epithelialisation

et al. 2016

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Hollow, tubular organs including oesophagus, trachea, stomach, intestine, bladder and urethra may require repair or replacement due to disease. Current treatment is considered an unmet clinical need, and tissue engineering strategies aim to overcome these by fabricating synthetic constructs as tissue replacements. Smart, functionalised synthetic materials can act as a scaffold base of an organ and multiple cell types, including stem cells can be used to repopulate these scaffolds to replace or repair the damaged or diseased organs. Epithelial cells have not yet completely shown to have efficacious cell–scaffold interactions or good functionality in artificial organs, thus limiting the success of tissue-engineered grafts. Epithelial cells play an essential part of respective organs to maintain their function. Without successful epithelialisation, hollow organs are liable to stenosis, collapse, extensive fibrosis and infection that limit patency. It is clear that the source of cells and physicochemical properties of scaffolds determine the successful epithelialisation. This article presents a review of tissue engineering studies on oesophagus, trachea, stomach, small intestine, bladder and urethral constructs conducted to actualise epithelialised grafts.

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“…Among them, 4% sodium deoxycholate (SDC) in combination with DNase is the most commonly used. [4][5][6][7][8][9][10][11][12] Some authors have reported acceptable degrees of decellularization using other detergents such as sodium dodecyl sulfate (SDS), Triton X-100 or 3-((3-cholamidopropyl) dimethylammonio)-1-propanesulfonate (CHAPS) alone or in combination. [13][14][15][16][17][18][19][20] However, in many cases the authors have directly extrapolated the decellularization protocols reported in the literature, without considering important aspects such as differences in the composition of the extracellular matrix that exist between species.…”

Section: Introductionmentioning

confidence: 99%

Optimization of a decellularization protocol of porcine tracheas. Long-term effects of cryopreservation. A histological study

et al. 2021

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Objective: The aim of this study was to optimize a decellularization protocol in the trachea of Sus scrofa domestica (pig) as well as to study the effects of long-term cryopreservation on the extracellular matrix of decellularized tracheas. Methods: Porcine tracheas were decellularized using Triton X-100, SDC, and SDS alone or in combination. The effect of these detergents on the extracellular matrix characteristics of decellularized porcine tracheas was evaluated at the histological, biomechanical, and biocompatibility level. Morphometric approaches were used to estimate the effect of detergents on the collagen and elastic fibers content as well as on the removal of chondrocytes from decellularized organs. Moreover, the long-term structural, ultrastructural, and biomechanical effect of cryopreservation of decellularized tracheas were also estimated. Results: Two percent SDS was the most effective detergent tested concerning cell removal and preservation of the histological and biomechanical properties of the tracheal wall. However, long-term cryopreservation had no an appreciable effect on the structure, ultrastructure, and biomechanics of decellularized tracheal rings. Conclusion: The results presented here reinforce the use of SDS as a valuable decellularizing agent for porcine tracheas. Furthermore, a cryogenic preservation protocol is described, which has minimal impact on the histological and biomechanical properties of decellularized porcine tracheas.

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Optimization of a Method for Preparation and Repopulation of the Tracheal Matrix for Allogenic Transplantation

Cited by 6 publications

References 13 publications

Towards reconstruction of epithelialized cartilages from autologous adipose tissue-derived stem cells

Towards reconstruction of epithelialized cartilages from autologous adipose tissue-derived stem cells

Tubular organ epithelialisation

Optimization of a decellularization protocol of porcine tracheas. Long-term effects of cryopreservation. A histological study

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