Optimization of a locomotion-based zebrafish seizure model

Shaw, Philip Anthony Gilbert; Panda, Siva Prasad; Stanca, Alexandru; Luyten, Walter

doi:10.1016/j.jneumeth.2022.109594

Cited by 5 publications

(11 citation statements)

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“…Also, they can react to visual and acoustic stimuli [9]. As zebrafish larva starts to feed at 5 days post fertilization (dpf) [10] and according to regulations the nonfeeding larva is not considered an animal, thus ethical permit is not required for performing experiments on � 5dpf zebrafish larvae. Hence zebrafish larvae can be considered as a non-animal in vivo vertebrate model.…”

Section: Introductionmentioning

confidence: 99%

“…Lower concentrations of PTZ can increase locomotion activity and high concentrations of PTZ (15 mM) cause a clonus-like convulsion that can only be reversed by some anti-epileptic drugs [2]. PTZ in 10 mM concentration is ideal for pharmacological tests and the behavioral effects of this concentration can last for approximately 16 hours [10]. The PTZ treatment under different illuminations also alters the anxiety level of zebrafish larvae differently, and the pattern of triggered movement abnormalities has not yet been investigated [3,16].…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Details of the research methods of epilepsy model of zebrafish are often not sufficiently described [10]. Tracking time of movements and duration time of recording vary in different publications [10]. Only a few studies focused on the methodology have been published, which correlate to specific locomotion detection techniques and drug concentration [3,10,16].…”

Section: Introductionmentioning

confidence: 99%

“…Tracking time of movements and duration time of recording vary in different publications [10]. Only a few studies focused on the methodology have been published, which correlate to specific locomotion detection techniques and drug concentration [3,10,16]. For the zebrafish epilepsy model refinement and standardization further experiments are needed [22].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

A comparative study to optimize experimental conditions of pentylenetetrazol and pilocarpine-induced epilepsy in zebrafish larvae

Szep,

Dittrich,

Gorbe

et al. 2023

PLoS ONE

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A common way to investigate epilepsy and the effect of antiepileptic pharmaceuticals is to analyze the movement patterns of zebrafish larvae treated with different convulsants like pentylenetetrazol (PTZ), pilocarpine, etc. Many articles have been written on this topic, but the research methods and exact settings are not sufficiently defined in most. Here we designed and executed a series of experiments to optimize and standardize the zebrafish epilepsy model. We found that during the light and the dark trials, the zebrafish larvae moved significantly more in the light, independent of the treatment, both in PTZ and pilocarpine-treated and the control groups. As expected, zebrafish larvae treated with convulsants moved significantly more than the ones in the control group, although this difference was higher between the individuals treated with PTZ than pilocarpine. When examining the optimal observation time, we divided the half-hour period into 5-minute time intervals, and between these, the first 5 minutes were found to be the most different from the others. There were fewer significant differences in the total movement of larvae between the other time intervals. We also performed a linear regression analysis with the cumulative values of the distance moved during the time intervals that fit the straight line. In conclusion, we recommend 30 minutes of drug pretreatment followed by a 10-minute test in light conditions with a 5-minute accommodation time. Our result paves the way toward improved experimental designs using zebrafish to develop novel pharmaceutical approaches to treat epilepsy.

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Section: Introductionmentioning

confidence: 99%