Optimization for Production of Intracellular Polysaccharide from Cordyceps ophioglossoides L2 in Submerged Culture and Its Antioxidant Activities in vitro

Xu, Qinqin; Liu, Zhenhua; Sun, Yisheng; Ding, Zhongjie; Lü, Longxian; Li, Yongquan

doi:10.1016/s1004-9541(12)60391-7

Cited by 13 publications

(12 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Link and other ascomycetes. The reason for this is low oxygen requirement by the culture at 150 rpm rotation [27, 37]. The results obtained under present experiments on the effect of rotation speed on polysaccharide production were the same as those obtained in C. ophioglossoides [37].…”

Section: Resultssupporting

confidence: 72%

“…C. gracilis incubated for 6 days and pH 6.0 showed maximum EPS and IPS production (Table 1). In the species, namely, C. ophioglossoides and C. sinensis, incubation period of 5-6 days and slightly acidic pH 5.0–6.0 promoted maximum IPS production [37, 46]. C. gracilis culture showed maximum EPS (405.24 ± 1.11 mg/L) and IPS (215.60 ± 1.71 mg/L) production at 23°C.…”

Section: Resultsmentioning

confidence: 99%

“…Subculturing was done in every 45-day interval to maintain them viable. Submerged culturing was done in a standard basal medium (saccharine 30.0 g/L, yeast powder 5.0 g/L, peptone 5.0 g/L, MgSO 4 ·7H 2 O 1.0 g/L, and KH 2 PO 4 0.5 g/L) [37, 38]. Effect of medium capacity (50, 100, 150, 200, and 250 mL), rotation speed (50, 100, 125, 150, 175, and 200 rpm) of culture medium, incubation time (2–10 days), pH (3.0–8.0), temperature (20, 23, 25, 27, 30, and 33°C), carbon sources (glucose, galactose, sucrose, mannitol, maltose, and fructose), nitrogen sources (yeast extract, peptone, NaNO 2 (NH 4 ) 2 SO 4 , and L-arginine HCL), and C/N (1 : 5, 1 : 10, 1 : 20, 1 : 30, and 1 : 40) ratio on EPS and IPS production was studied by orthogonal experiments using one-factor-at-a-time method.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Optimization, Composition, and Antioxidant Activities of Exo- and Intracellular Polysaccharides in Submerged Culture ofCordyceps gracilis(Grev.) Durieu & Mont.

Sharma

Gautam

Atri

2015

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Under present experiments, EPS and IPS production, monosaccharide composition, and antioxidant activities of C. gracilis were studied for the first time under submerged culture conditions. Effect of different factors on polysaccharides production was studied by orthogonal experiments using one-factor-at-a-time method. Incubation of culture in the medium with capacity 200 mL (675.12 ± 5.01 and 385.20 ± 5.01 mg/L), rotation speed 150 rpm (324.62 ± 3.32 and 254.62 ± 4.62 mg/L), 6-day culture incubation time (445.24 ± 1.11, 216.60 ± 1.71 mg/L), pH 6.0 (374.81 ± 2.52 and 219.45 ± 2.59 mg/L), and temperature 23°C (405.24 ± 1.11 and 215.60 ± 1.71 mg/L) produced higher EPS and IPS, respectively. Maximum EPS and IPS production was observed in the medium supplemented with glucose as a carbon source (464.82 ± 2.12 and 264.42 ± 2.62 mg/L) and yeast extract as a nitrogen source (465.21 ± 3.11 and 245.17 ± 3.24 mg/L), respectively. Carbon to nitrogen ratio for maximum EPS and IPS production was observed as 10 : 1 (395.29 ± 2.15 and 235.62 ± 1.40 mg/L), respectively. Glucose was found to be the major monosaccharide (62.15 ± 7.33%). Both EPS and IPS of C. gracilis showed significant DPPH radical scavenging activity, ABTS radical scavenging activity, reducing power, and iron chelating activity.

show abstract

Section: Resultssupporting

confidence: 72%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Optimization, Composition, and Antioxidant Activities of Exo- and Intracellular Polysaccharides in Submerged Culture ofCordyceps gracilis(Grev.) Durieu & Mont.

Sharma

Gautam

Atri

2015

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

show abstract

“…One another study of [29] IPS exhibited scavenging activity on hydroxyl radical in a concentration dependent manner. The scavenging ratios were improved with the increase of extracts concentration.…”

Section: Discussionmentioning

confidence: 94%

Antioxidant potentials of aqueous and acetone ips extracts of gracilaria crassa

2017

ajbpr

View full text Add to dashboard Cite

Abstract:With the increasing demand of functional foods with antioxidant properties it is necessary to establish new sources such as macro algae which could provide beneficial effects to human health. The present study was evaluated for antioxidant potentials of Gracilaria crassa. Results revealed that Gracilaria crassa significantly recorded the highest IC50 value in acetone than aqueous extracts. Hence it is noteworthy that both aqueous and acetone IPS extracts of Gracilaria crassa hold promising antioxidant potentials.

show abstract

“…Our lab has purified and identified the fungus from fruit body of C. ophioglossoides and set up an artificial fermentation system of it. 22,23) Previously, we have reported the discovery of a novel spiro [4.5] decane sesquiterpene, cordycepol C, isolated from the cultured mycelia of C. ophioglossoides. Cordycepol C contains a hydroperoxy group and is cytotoxic to HepG2 cells.…”

mentioning

confidence: 99%

Cordycepol C Induces Caspase-Independent Apoptosis in Human Hepatocellular Carcinoma HepG2 Cells

Sun

Zhao

et al. 2014

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

Cordycepol C, a novel sesquiterpene isolated from the cultured mycelia of Cordyceps ophioglossoides, contains a hydroperoxy group and is cytotoxic to HepG2 cells. So far, no sesquiterpenes have been found in the genus Cordyceps and it would be interesting to investigate the antitumor efficacy as well as the mechanism of action of this unusual sesquiterpene. In this study, we showed that cordycepol C induced apoptosis of the HepG2 cells without affecting the normal liver cell line L-02. Cordycepol C caused poly(ADP-ribose) polymerase-1 (PARP-1) cleavage and triggered the loss of mitochondrial membrane potential (Δψ m ) in HepG2 cells in a time-and dose-dependent manner, resulting in the nuclear translocation of apoptosis-inducing factor (AIF) and endonuclease G (Endo G). We also found that cordycepol C induced the expression of Bax protein, followed by its translocation from the cytosol to mitochondria in both wild type and p53 knockdown HepG2 cells. However, cordycepol C could not cause cleavages of procaspase-3, -8, and -9. Caspase activities were not increased and Z-VAD-fmk, a caspase inhibitor, could not prevent the apoptosis induced by cordycepol C. These findings indicate that cordycepol C induces caspase-independent apoptosis in HepG2 cells through a p53-independent and Bax-mediated mitochondrial pathway, leading to the nuclear translocation of AIF and Endo G. Our study provides the molecular mechanism by which cordycepol C induces apoptosis in hepatocellular carcinoma cells and indicates the potential use of cordycepol C as an antitumor agent.

show abstract

Optimization for Production of Intracellular Polysaccharide from Cordyceps ophioglossoides L2 in Submerged Culture and Its Antioxidant Activities in vitro

Cited by 13 publications

References 39 publications

Optimization, Composition, and Antioxidant Activities of Exo- and Intracellular Polysaccharides in Submerged Culture ofCordyceps gracilis(Grev.) Durieu & Mont.

Optimization, Composition, and Antioxidant Activities of Exo- and Intracellular Polysaccharides in Submerged Culture ofCordyceps gracilis(Grev.) Durieu & Mont.

Antioxidant potentials of aqueous and acetone ips extracts of gracilaria crassa

Cordycepol C Induces Caspase-Independent Apoptosis in Human Hepatocellular Carcinoma HepG2 Cells

Contact Info

Product

Resources

About