Optimization for biodegradation of 2,4,6-trinitrotoluene (TNT) by Pseudomonas putida

Park, Chulhwan; Kim, Tak Hyun; Kim, Sang Yong; Kim, Seung Wook; Lee, Jinwon; Kim, Sun Hwan

doi:10.1016/s1389-1723(03)80163-9

Cited by 42 publications

(23 citation statements)

References 14 publications

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“…The optimal value of kinetic constant average for asphaltene biodegradation was 0.138 g/ (L day) for B. lentus [17]. The optimal biokinetic constant of Pseudomonas putida was 0.16 g/ (L day) for the biodegradation of TNT [18]. The motor oil biodegradation capacity of Pseudomonas aeruginosa was enhanced from 0.178 g/ (L day) to 0.34 g/ (L day) in optimum conditions [19].…”

Section: Resultsmentioning

confidence: 99%

“…A consortium of the mixed facultative anaerobes Acinetobacter calcoaceticus and Pantoea agglomerans highly degraded LAS and SDS at pH 8.5 [20]. Optimal pH in various processes were 6 for biodegradation of 4-chlorophenol by Candida tropicalis PHB5 [21], 6.7 for asphaltene biodegradation by pure cultures of B. lentus [17], and 7 for the biodegradation of TNT by Pseudomonas putida [18]. The optimal growth and biomass yields occurred at pH 6.8 in chemostat [22].…”

Section: Resultsmentioning

confidence: 99%

“…LAS (700 ppm) and SDS (8000 ppm) as surfactants at higher concentrations became toxic to the A. calcoaceticus [20]. The optimal tween80 concentration for the biodegradation of TNT by Pseudomonas putida was 0.1% [18]. Thus, according to microorganism and substrate, amount and type of a surfactant can be changed into produce biomass or raise kinetic constant.…”

Section: Resultsmentioning

confidence: 99%

“…For biodegradation of crude oil concentration of 1 g/L, optimal nitrogen was supplemented with 190.21 mg/L [25]. The optimum NH 4 Cl as a nitrogen source was 0.025% for the biodegradation of TNT by Pseudomonas putida [18]. Optimum NH 4 Cl for BTEX degradation was found as 1.484 g/L [26].…”

Section: Resultsmentioning

confidence: 99%

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Optimization of biomass and biokinetic constant in Mazut biodegradation by indigenous bacteria BBRC10061

Khorasani

Mashreghi

Yaghmaei

2014

J Environ Health Sci Engineer

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Optimization based on appropriate parameters can be applied to improve a process. Mazut degradation as a critical issue in environment requires optimization to be efficiently done. To provide biodegradation conditions, experiments were designed on the least interactions among levels of parameters consisting of pH, Tween 80, glucose, phosphorous source, nitrogen source, and time. Kinetic constants and biomass were calculated based on 16 assays, designed using Taguchi method, which constructed various mazut biodegradation conditions. Kinetics of mazut degradation by newly isolated bacteria Enterobacter cloacae closely followed second order kinetic model. Results of the 16 experiments showed that biomass was in the range of 0.019 OD600 to 2.75 OD600, and biokinetic constant was in the range of 0.2 × 10-5 L/ (mg day) to 10-4 L/ (mg day). Optimal level for each parameter was obtained through data analysis. For optimal biomass equal to 2.75 OD600, optimal pH, Tween80, glucose, phosphorous source, and time were 8.3, 4 g/L, 4 g/L, 9 g/L, and 10 days, respectively. For biokinetic constant equal to 1.2 × 10-4 L/ (mg day), optimal pH, Tween80, glucose, phosphorous source, and nitrogen source were 8.3, 1 g/L, 4 g/L, 1 g/L, and 9 g/L, respectively. The optimum levels for biomass and biokinetic constant were the same except the levels of the Tween 80, and phosphorous source. Consequently, mazut may be more degraded with adjusting the conditions on the optimum condition.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Optimization of biomass and biokinetic constant in Mazut biodegradation by indigenous bacteria BBRC10061

Khorasani

Mashreghi

Yaghmaei

2014

J Environ Health Sci Engineer

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“…2,4,6-Trinitrotoluene (TNT) is one of the most frequently used organic high explosive by the armed forces and terrorist groups around the world. Military activities like training, testing, mining, dismantling, open burning/open detonation, and wars have caused widespread contamination of soil and water with TNT and its degradation products [1,2]. Major route of entry of TNT to surface water and thus food chain is through discharge of waste streams generated during its manufacturing Abbreviations: 1,3,5-TNB, 1,3,5-trinitrobenzene; 1,3-DNB, 1,3-dinitrobenzene; 2-Am-4,6-DNT, 2-amino-4,6-dinitrotoluene; 4-Am-2,6-DNT, 4-amino-2,6-dinitrotoluene; BIN, Barrel Insert and Needle Assembly; DNT, dinitrotoluene; LLE, liquid-liquid extraction; MEPS, microextraction by packed sorbent; NB, nitrobenzene; NT, nitrotoluene; SIM, selected ion monitoring; Tetryl, 2,4,6-trinitrophenyl-N-methylnitramine; TNT, 2,4,6-trinitrotoluene Conflict of interest: The authors have declared no conflict of interest.…”

Section: Introductionmentioning

confidence: 99%

Development of a microextraction by packed sorbent with gas chromatography‐mass spectrometry method for quantification of nitroexplosives in aqueous and fluidic biological samples

Dhingra

Bansal

Dhingra³

et al. 2018

J of Separation Science

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A new method for quantification of 12 nitroaromatic compounds including 2,4,6-trinitrotoluene, its metabolites and 2,4,6-trinitrophenyl-N-methylnitramine with microextraction by packed sorbent followed by gas chromatography and mass spectrometric detection in environmental and biological samples is developed. The microextraction device employs 4 mg of C silica sorbent inserted into a microvolume syringe for sample preparation. Several parameters capable of influencing the microextraction procedure, namely, number of extraction cycles, washing solvent, volume of washing solvent, elution solvent, volume of eluting solvent and pH of matrix, were optimized. The developed method produced satisfactory results with excellent values of coefficient of determination (R > 0.9804) within the established calibration range. The extraction yields were satisfactory for all analytes (> 89.32%) for aqueous samples and (> 87.45%) for fluidic biological samples. The limits of detection values lie in the range 14-828 pg/mL.

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Aerobic degradation of 2,4‐dinitrotoluene: Effect of raw organic wastes and nitrogen fortification

2020

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Abstract2,4‐Dinitrotoluene (2,4‐DNT), a principal derivative generated in the synthesis of 2,4,6‐trinitrotoluene, is widely used as a waterproofer, plasticizer, and gelatinizer in propellants and explosives. This compound has been documented as a priority pollutant because of its toxicity. Therefore, its removal from contaminated systems is a major focus of research and environmental attention. The presence of 2,4‐DNT bacterial‐degrading strains that could utilize 2,4‐DNT as growth substrate in polluted sites in Ibadan, Nigeria, was determined using continual enrichment techniques on nitroaromatic mixtures. Proteus sp. strain OSES2 isolated in this study was characterized by phenotypic typing and 16S ribosomal RNA gene sequencing. Growth of the strain on 2,4‐DNT resulted in an exponential increase in biomass and complete substrate utilization within 72 h, accompanied by NO3− elimination. Degradation competence was enhanced in the presence of corn steep liquor, molasses, and Tween 80 compared with incubation without amendment. Conversely, amendment with nitrogen sources yielded no significant improvement in degradation. Use of these organic wastes as candidates in a bioremediation strategy should be exploited. This would provide a less‐expensive organic source supplement for cleanup purposes, with the ultimate aim of reducing the cost of bioremediation while reducing wastes intended for landfill.

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Optimization for biodegradation of 2,4,6-trinitrotoluene (TNT) by Pseudomonas putida

Cited by 42 publications

References 14 publications

Optimization of biomass and biokinetic constant in Mazut biodegradation by indigenous bacteria BBRC10061

Optimization of biomass and biokinetic constant in Mazut biodegradation by indigenous bacteria BBRC10061

Development of a microextraction by packed sorbent with gas chromatography‐mass spectrometry method for quantification of nitroexplosives in aqueous and fluidic biological samples

Aerobic degradation of 2,4‐dinitrotoluene: Effect of raw organic wastes and nitrogen fortification

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