Optimization Conditions of Extracellular Proteases Production from a Newly Isolated Streptomyces Pseudogrisiolus NRC‐15

Abstract

Microbial protease represents the most important industrial enzymes, which have an active role in biotechnological processes. The objective of this study was to isolate new strain ofStreptomycesthat produce proteolytic enzymes with novel properties and the development of the low-cost medium. An alkaline protease producer strain NRC-15 was isolated from Egyptian soil sample. The cultural, morphological, physiological characters and chemotaxonomic evidence strongly indicated that the NRC-15 strain represents a novel species of the genusStreptomyces, hence the nameStrptomyces pseudogrisiolusNRC-15. The culture conditions for higher protease production by NRC-15 were optimized with respect to carbon and nitrogen sources, metal ions, pH and temperature. Maximum protease production was obtained in the medium supplemented with 1% glucose, 1% yeast extract, 6% NaCl and 100 μmol/L of Tween 20, initial pH 9.0 at 50 °C for 96 h. The current results confirm that for this strain, a great ability to produce alkaline proteases, which supports the use of applications in industry.

An Alkaline Protease from Bacillus pumilus MP 27: Functional Analysis of Its Binding Model toward Its Applications As Detergent Additive

“…Further the interaction between the surfactant and carbon source is of relevance, where, the lower level in the carbon source was balanced by higher levels of surfactant for the establishment of worthy protease activity. This might be due to the adsorbed surfactant film around the cell which decreased or increased permeability or enhanced the availability of important ions, which has a favorable effect on protease activity ( Mostafa et al, 2012 ). Thus, from the present study it can be concluded that protease production in B. pumilus can be improved by controlling the carbon source and surfactants and this interaction could only be well-understood by suitable RSM selection.…”

Production and Characterization of Extremophilic Proteinases From a New Enzyme Source, Barrientosiimonas sp. V9

“…For protease production, a 2% (v/v) inoculum was used in 500 mL (1:10 medium:flask volume ratio) at initial pH 7.0. The initial evaluation was carried out using basal media previously reported for protease production [17][18][19][20][21], as detailed in Table 1. Further evaluation was performed with different carbon (10 g L −1 glucose, sucrose, glycerol, fructose, maltose, lactose, galactose, cellobiose, starch, cane molasses) or nitrogen sources (10 g L −1 peptone, yeast extract, skim milk, NH 4 Cl, (NH 4 ) 2 SO 4 , casein hydrolysate, beef extract, urea, gelatin, casein) by substituting original C or N sources in the selected basal medium B for protease production.…”

O Potencial Do Streptomyces Isolados Na Região De Maués Na Produção De Enzimas Hidrolíticas

“…Os grupos das proteases constituem o maior grupo de enzimas utilizadas industrialmente no mundo, movimentando a economia deste setor ativamente (PILLAI et al, 2011). Estas enzimas têm como função hidrolisar ligações peptídicas (LI et al, 2012) e podem ser obtidas de diferentes fontes, entretanto as proteases microbianas se destacam pela sua metabolização e baixo custo (AWAD et al, 2013;MOSTAFA et al, 2012).…”

“…Os grupos das proteases constituem o maior grupo de enzimas utilizadas industrialmente no mundo, movimentando a economia deste setor ativamente (PILLAI et al, 2011). Estas enzimas têm como função hidrolisar ligações peptídicas (LI et al, 2012) e podem ser obtidas de diferentes fontes, entretanto as proteases microbianas se destacam pela sua metabolização e baixo custo (AWAD et al, 2013;MOSTAFA et al, 2012).As amilases estão entre as mais importantes enzimas industriais, são aplicadas como aditivos em detergentes, indústrias de alimentos, fermentação, papel e têxtil, farmacêutica, médica e químico-analítica (PANDEY et al, 2000; SOARES et al, 2010).As enzimas lipolíticas catalisam a hidrólise de gorduras e óleos, são consideradas o terceiro maior grupo baseado no volume total de compras, onde o uso comercial foi estimado em um bilhão (JAEGER et al, 1999). Possuem diversas aplicações industriais, utilizadas como biocatalisadores ideais em química orgânica entre outros (TREICHEL et al, 2010;LOU et al, 2013;SHARMA; KANWAR, 2014).…”