Optimization, antioxidant potential, modulatory effect and anti-apoptotic action in of Euphorbia bivonae polysaccharides on hydrogen peroxide-induced toxicity in human embryonic kidney cells HEK293

Athmouni, Khaled; Belhaj, Dalel; Feki, Abdelfattah El; Ayadi, Habib

doi:10.1016/j.ijbiomac.2018.04.172

Cited by 9 publications

(7 citation statements)

References 33 publications

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“…The results showed that the half maximal inhibitory concentration (IC50) of H2O2 was indicated as 1.57 mmole/L (R 2 = 0.9972). Previously reported [25], H2O2 concentration 2 mmole/L was affected to 50 % cell viability of HEK-293 cells which was close to our experiment of H2O2 concentration. Oxidative stress has been proposed as "an imbalance between oxidants and antioxidants in favor of the oxidants, leading to a disruption of redox signaling and control and/or molecular damage" [46].…”

Section: Effect Of Mjp On H2o2-induced Toxicity In Hek-293 Cellssupporting

confidence: 92%

“…This finding reported [30] that the HEK-293 cells of Thai fruits juice significantly decreased the ROS level. Similar to present study [25] found that Euphorbia bivonae polysaccharides diminished ROS production in HEK-293 cells compare with only H2O2 induced cells.…”

Section: Effect Of Mjp On Intracellular Ros Productionsupporting

confidence: 92%

“…In addition, lipid peroxidation was expressed as MDA contents (by product of lipid peroxidation). The results showed that significantly (p < 0.01) increased MDA of 72.23% in H2O2 treated group, but in the co-treatment with 0.1-10 mg/mL MJP significantly lower MDA contents than H2O2 treated group of 11.74 to 34.71% (Figure 5c) [25,29,30]. The most important protection mechanisms against the free radicals or oxidants in the body are endogenous intracellular antioxidant enzymes such as superoxide dismutase (SOD) changes superoxide radical (O2 •-. )…”

Section: Effect Of Mjp On Enzymatic Antioxidant Activity and Lipid Peroxidationmentioning

confidence: 91%

“…Cytotoxicity of MJP was determined by the MTT assay according to the method previously [25] with some modifications. Cells were seeded in 96-well culture plates at a density of 5×10 4 cells/well (n=3).…”

Section: Determination Of Cytotoxicity Of Mjpmentioning

confidence: 99%

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Evaluation on antioxidative capacity, nephroprotective effect, and DNA damage protection of mixed low potassium vegetables and fruits juice powder in HEK-293 cells

Khamhae

Kanokwiroon

Chansuwan

et al. 2020

FFHD

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Background: An imbalance between free radical production and antioxidant defenses is one of the multifactorial natures contributing to many disorders. One of them is chronic kidney disease (CKD). The development of novel diets to slow progression or reduces complication of CKD are highly needed. Therefore, the objective of this study was to evaluate the in vitro antioxidative capacity and nephroprotective effect of a product, mixed low potassium vegetables and fruits juice powder (MJP) in HEK-293 cells.Methods: The produced MJP was determined for phenolics and flavonoids content. The antioxidative capacity was examined by DPPH, FRAP and ABTS assay. The cell viability to MJP and hydrogen peroxide (H2O2)-treated were determined by MTT assay. The intracellular antioxidative capacity was determined by co-treatment of MJP and H2O2-induced HEK-293 cell damage including reactive oxygen species (ROS) production, intracellular enzymes activities, and DNA damage protection.Results: The MJP at the concentration of 5 and 10 mg/ml showed positive effects on H2O2-induced HEK-293 cells protection and significantly increased cell viability. The ROS production measured by 2’,7’-dichlorodihydrofluorescein diacetate (DCF) were increased in the H2O2 treatment approximately 2 times but significantly decreased in the MJP treatment. The result of intracellular enzymatic antioxidant markers, including superoxide dismutase (SOD) and catalase (CAT) activities were increased while the Malonaldehyde (MDA) level was attenuated in the co-treatment of MJP and H2O2-induced HEK293 cells compared with only H2O2-inducing. In the examination of the morphological change due to oxidative stress exhibited less nuclei fragment in the cell of MJP treatment. In addition, the isolated DNA was protected by MJP application. Conclusion: The produced MJP contains bioactive phenolic and flavonoid compounds. MJP possesses potential antioxidative capacity through the reducing H2O2-induced HEK-293 cells damage and increasing the intracellular antioxidant enzymes. In addition, the nephroprotective effects of MJP related to the antioxidant enzymes was involved in the free radical scavenging. Therefore, MJP could be a functional drink for individual limiting potassium and liquid uptake.Keywords: Antioxidative capacity, DNA damage protection, low potassium vegetables and fruits, nephroprotective, chronic kidney disease

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Section: Effect Of Mjp On H2o2-induced Toxicity In Hek-293 Cellssupporting

confidence: 92%

Section: Effect Of Mjp On Intracellular Ros Productionsupporting

confidence: 92%

Section: Effect Of Mjp On Enzymatic Antioxidant Activity and Lipid Peroxidationmentioning

confidence: 91%

Section: Determination Of Cytotoxicity Of Mjpmentioning

confidence: 99%

See 2 more Smart Citations

Evaluation on antioxidative capacity, nephroprotective effect, and DNA damage protection of mixed low potassium vegetables and fruits juice powder in HEK-293 cells

Khamhae

Kanokwiroon

Chansuwan

et al. 2020

FFHD

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show abstract

“…Moreover, as most of the synthetic antioxidants are potentially harmful to humans, it is particularly important to seek natural antioxidants instead. In fact, most of the polysaccharides extracted from natural resources present low toxicity and exhibit a strong biological activity when compared with other natural antioxidants [20]. It should be noted that many plant-based products rich in polysaccharides, such as that from Astragalus membranaceus, exist in the market and are commercialized for their health-related properties.…”

Section: Introductionmentioning

confidence: 99%

Water-Soluble Polysaccharides from Ephedra alata Stems: Structural Characterization, Functional Properties, and Antioxidant Activity

et al. 2020

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In this study, the physicochemical characterization, functional properties, and antioxidant activity of polysaccharides extracted from Ephedra alata (EAP) were investigated. EAP were extracted in water during 3 h with a liquid/solid ratio of 5 in a water bath at 90 °C. The structure of the extracted EAP was examined by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), and gas chromatography-mass spectrometry (GC-MS). The functional properties and biochemical activities of EAP were determined. The chemical analysis revealed that the contents of carbohydrates, uronic acid, and proteins were 73.24% ± 1.24%, 6.82% ± 0.57%, and 6.56% ± 0.36%, respectively. The results showed that the extracted EAP essentially contain three functional groups: C=O, C-H, and O-H. SEM images showed that EAP present numerous high porosity particles. The monosaccharide composition revealed a polymer composed of glucose (43.1%), galactose (36.4%), mannose (14.9%), arabinose (3.7%), and gluconic acid (1.7%). EAP showed interesting functional properties (solubility, oil holding capacity, foaming and emulsion properties). Finally, the results revealed that EAP displayed excellent antihypertensive and antioxidant activities. Overall, EAP present a promising natural source of food additives, antioxidants, and antihypertensive agents.

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Plant flavonoids bioavailability in vivo and mechanisms of benefits on chronic kidney disease: a comprehensive review

et al. 2022

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Optimization, antioxidant potential, modulatory effect and anti-apoptotic action in of Euphorbia bivonae polysaccharides on hydrogen peroxide-induced toxicity in human embryonic kidney cells HEK293

Cited by 9 publications

References 33 publications

Evaluation on antioxidative capacity, nephroprotective effect, and DNA damage protection of mixed low potassium vegetables and fruits juice powder in HEK-293 cells

Evaluation on antioxidative capacity, nephroprotective effect, and DNA damage protection of mixed low potassium vegetables and fruits juice powder in HEK-293 cells

Water-Soluble Polysaccharides from Ephedra alata Stems: Structural Characterization, Functional Properties, and Antioxidant Activity

Plant flavonoids bioavailability in vivo and mechanisms of benefits on chronic kidney disease: a comprehensive review

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