Optimization and Validation of RP-LC/UV–VIS Detection Method for Simultaneous Determination of Fat-Soluble Anti-Oxidant Vitamins, all-trans-Retinol and α-Tocopherol in Human Serum: Effect of Experimental Parameters

Khan, Muhammad Imran; Khan, Abad; Iqbal, Zafar; Ahmad, Lateef; Shah, Yatrik M.

doi:10.1365/s10337-010-1486-0

Cited by 16 publications

(17 citation statements)

References 35 publications

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“…The chromatographic method was validated according to international guidelines with emphasis on specificity/ selectivity, linearity within the expected concentration range, recovery, precision (repeatability and intermediate precision), sensitivity, stability of solutions, and robustness [24,25].…”

Section: Methods Validationmentioning

confidence: 99%

Simultaneous Determination of the Endogenous Free α-Lipoic Acid and Dihydrolipoic Acid in Human Plasma and Erythrocytes by RP-HPLC with Electrochemical Detection

et al. 2011

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A highly sensitive, precise, and accurate reversed-phase high-performance liquid-chromatography/ electrochemical detection method for simultaneous determination of the endogenous free a-lipoic acid and dihydrolipoic acid in biological matrices was developed and validated. The two analytes were extracted from the samples with acetonitrile/10% metaphosphoric acid solution (aqueous) (50/50 v/v). To determine the total lipoic acid, samples were treated with tris(2-carboxyethyl)phosphine solution in phosphate buffer, pH 2.5 with 85% orthophosphoric acid prior to deproteination. The two analytes were separated on a C 18 (150 9 4.6 mm, 5 lm) analytical column using acetonitrile-50 mM phosphate buffer, pH 2.5 with 85% orthophosphoric acid (35/65 v/v) as the isocratic mobile phase pumped at a flow rate of 2.0 mL min -1 at the column oven temperature of 35°C. The column eluents were monitored at a potential of 0.9 V. These analytes were efficiently resolved in \7 min. The present method was sufficiently robust and specific for simultaneous determination of the two analytes and demonstrated acceptable values for linearity (r 2 = 0.999 in the range of 0.1-500 and 0.25-1,000 ng mL -1 for a-lipoic acid and dihydrolipoic acid, respectively), recovery ([97%), precision (RSD% \2), and sensitivity (on column limit of detection, 150 and 375 fg for a-lipoic acid and dihydrolipoic acid, respectively and limit of quantification: 0.5 and 1.25 pg for a-lipoic acid and dihydrolipoic acid, respectively), indicating that the proposed method was more sensitive, precise, economical, and versatile, and has higher throughput than the previously reported methods for simultaneous determination of the two analytes.

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Section: Methods Validationmentioning

confidence: 99%

Simultaneous Determination of the Endogenous Free α-Lipoic Acid and Dihydrolipoic Acid in Human Plasma and Erythrocytes by RP-HPLC with Electrochemical Detection

et al. 2011

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“…The proposed analytical method was validated according to international guidelines with emphasis on selectivity, linearity within the expected concentration range, recovery, precision (repeatability and intermediate precision), sensitivity, stability of solutions, and robustness [21,22].…”

Section: Methods Validationmentioning

confidence: 99%

Simultaneous determination of cefdinir and cefixime in human plasma by RP-HPLC/UV detection method: Method development, optimization, validation, and its application to a pharmacokinetic study

Khan

Iqbal

Khan

et al. 2011

Journal of Chromatography B

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“…(HPLC) [22][23][24][25][26][27][28][29][30][31][32]. The detectors most often used are the ultraviolet detector (UVD) [22], fluorescence detector (FLD) [23], electrochemical detector (ECD) [24], and mass spectrometer (MS) [25].…”

mentioning

confidence: 99%

“…The detectors most often used are the ultraviolet detector (UVD) [22], fluorescence detector (FLD) [23], electrochemical detector (ECD) [24], and mass spectrometer (MS) [25]. Tedious derivatization procedures are usually required before GC, and CE lacks high sensitivity.…”

mentioning

confidence: 99%

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Simultaneous HPLC–DAD Determination of Retinol and Eight Vitamin E Isomers in Human Serum

Yang

Zhang

et al. 2015

Chromatographia

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Optimization and Validation of RP-LC/UV–VIS Detection Method for Simultaneous Determination of Fat-Soluble Anti-Oxidant Vitamins, all-trans-Retinol and α-Tocopherol in Human Serum: Effect of Experimental Parameters

Cited by 16 publications

References 35 publications

Simultaneous Determination of the Endogenous Free α-Lipoic Acid and Dihydrolipoic Acid in Human Plasma and Erythrocytes by RP-HPLC with Electrochemical Detection

Simultaneous Determination of the Endogenous Free α-Lipoic Acid and Dihydrolipoic Acid in Human Plasma and Erythrocytes by RP-HPLC with Electrochemical Detection

Simultaneous determination of cefdinir and cefixime in human plasma by RP-HPLC/UV detection method: Method development, optimization, validation, and its application to a pharmacokinetic study

Simultaneous HPLC–DAD Determination of Retinol and Eight Vitamin E Isomers in Human Serum

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