Simultaneous HPLC–DAD Determination of Retinol and Eight Vitamin E Isomers in Human Serum

Yang, Yi; Lu, Dan; Zhang, Jing; Li, Yongxin; Zheng, Bo; Sun, Chengjun

doi:10.1007/s10337-015-2951-6

Cited by 5 publications

(1 citation statement)

References 44 publications

(48 reference statements)

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“…Qualitative and quantitative determination of T and T3 in various matrices is usually performed with the application of fluorescence (FLD) (Chajès, Lhuillery, Sattler, Kostner, & Bougnoux, ; Handelman, Epstein, Machlin, van Kuijk, & Dratz, ; Rupérez, Barbas, Castro, Martínez, & Herrera, ; Shim et al, ; Takahashi et al, ; Weiser, Riss, & Kormann, ; Yeum et al, ) and diode‐array detection (Gleize, Steib, André, & Reboul, ; Yang et al, ; Yuan, Ju, Jin, Ren, & Liu, ). Mass spectrometry (MS) is rarely implemented in determination of T and T3 (Ansolin, de Souza, de Almeida Meirelles, & Batista, ; Bartosińska, Buszewska‐Forajta, & Siluk, ; Nagy et al, ; Viñas et al, ) as the ionization process of these compounds is problematic.…”

Section: Introductionmentioning

confidence: 99%

Determination of tocopherols and tocotrienols in human breast adipose tissue with the use of high performance liquid chromatography–fluorescence detection

Bartosińska

Jacyna

Moor

et al. 2018

Biomedical Chromatography

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Tocopherols and tocotrienols have been extensively studied owing to their anticancer potential, especially against breast cancer. Therefore, the aim of this study was to quantitatively determine tocochromanols in human breast adipose tissue with the use of HPLC-FLD. The sample preparation procedure included homogenization and solvent extraction with isopropanol-ethanol-0.1% formic acid mixture prior to solid-phase extraction. After implementation of central composite design, satisfactory separation of all eight target compounds was achieved within 10.5 min. Chromatographic runs were carried out with the use of a naphthylethyl chromatographic column with methanol-water mixture (89:11, v/v) as the mobile phase. Fluorescence detection of tocochromanols was performed with excitation and emission wavelengths 298 and 330 nm, respectively. The method was validated in terms of linearity, carryover, recovery, precision, accuracy and stability. Extraction yield was also determined for accurate evaluation of vitamin E content in human breast adipose tissue samples. Finally, concentrations of particular tocochromanols compounds were assessed in human breast adipose tissue samples obtained from 99 patients, including women with breast cancer, healthy volunteers and deceased women who had died as a result of accidents. The raw data was transformed according to the newly developed equation for accurate estimation of the concentrations of tocochromanols in breast adipose tissue samples. Results obtained in the study indicated that the proposed analytical assay could be useful in breast cancer research.

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Section: Introductionmentioning

confidence: 99%

Determination of tocopherols and tocotrienols in human breast adipose tissue with the use of high performance liquid chromatography–fluorescence detection

Bartosińska

Jacyna

Moor

et al. 2018

Biomedical Chromatography

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Determination of Five Retinol Isomers in Animal Livers Using Ultra-High Performance Supercritical Fluid Chromatography

Zhao

et al. 2018

Chromatographia

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A Novel Ionic Liquid-Based Liquid-Liquid Microextraction Combined with High Performance Liquid Chromatography for Simultaneous Determination of Eight Vitamin E Isomers in Human Serum

Yin

Yang

Zhang

et al. 2020

Journal of AOAC INTERNATIONAL

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Background Vitamin E deficiencies are prevalent around the world and have become one of the major public health issues. It is necessary to determine their levels in human serum for routine clinical practice. Objective In this study, a simple and green ionic liquid-based (IL)vortex-assisted (VA) liquid–liquid microextraction (LLME) combined with HPLC was developed for simultaneous determination of eight vitamin E isomers in human serum. Methods The IL, 1-octyl-methylimidazolium trifluoromethanesulfonate ([OMIM]OTf), was added into the diluted sample and vortexed to form a cloudy solution. After centrifugation, the IL phase was collected for HPLC analysis. The separation was accomplished on a Phenomenex Luna-C18 column (250 mm × 4.6 mm, 5 μm) and the column temperature was 30°C. The mobile phase was methanol/acetonitrile (80 + 20, v/v) and the flow rate was 0.7 mL/min. A fluorescence detector was used for the simultaneous detection of eight vitamin E isomers, and the detection wavelength was set at 290/327 nm. The LLME procedure can be completed within 10 min without using any organic solvent. The parameters affecting the extraction efficiencies were optimized, including the type and volume of the ILs, dispersive solvent, vortex time, and salt addition. Results Under the optimal conditions, limits of detection were 0.857–4.16 ng/mL. Acceptable recoveries ranging from 80.1% to 103% were achieved, with relative standard deviations less than 13.0%. The proposed method was successfully applied to the detection of eight vitamin E isomers in human serum samples. Conclusions This method is simple, fast, environment-friendly, cheap, and has similar linear ranges, sensitivities, accuracy, and precision as those reported chromatographic methods. Highlights The IL, [OMIM]OTf, was chosen as the green extractant of LLME for vitamin E extraction because of its strong adsorption property for vitamin E isomers. An IL-VA-LLME method has been developed for the analysis of 8 vitamin E isomers. The established method was successfully applied to the analysis of 8 vitamin E isomers in human serum samples.

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Simultaneous HPLC–DAD Determination of Retinol and Eight Vitamin E Isomers in Human Serum

Cited by 5 publications

References 44 publications

Determination of tocopherols and tocotrienols in human breast adipose tissue with the use of high performance liquid chromatography–fluorescence detection

Determination of tocopherols and tocotrienols in human breast adipose tissue with the use of high performance liquid chromatography–fluorescence detection

Determination of Five Retinol Isomers in Animal Livers Using Ultra-High Performance Supercritical Fluid Chromatography

A Novel Ionic Liquid-Based Liquid-Liquid Microextraction Combined with High Performance Liquid Chromatography for Simultaneous Determination of Eight Vitamin E Isomers in Human Serum

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