Optimization and validation of a method for the direct determination of catechin and epicatechin in red wines by HPLC/fluorescence

Dias, Fábio de S.; Palma, Miguel; Barroso, Carmelo Garcı́a; David, Jorge

doi:10.1016/j.microc.2010.01.004

Cited by 38 publications

(27 citation statements)

References 26 publications

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“…The LOD was 0.04-0.450 µg/ml on the foundation of s/n ratio of 3 and the LOQ was 3.3 times of LOD (Table 3, Supplementary Material C 1-2). Our study corroborates with the findings of Dias et al [30], Escarpa and González [31].…”

Section: Linearity Loq and Lodsupporting

confidence: 93%

Simultaneous Estimation of Twenty Eight Phenolic Compounds by a Novel and Expeditious Method Developed on Quaternary Ultra-Performance Liquid Chromatography System with a Photodiode Array Detector

2019

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Plant secondary metabolites including phenolics and flavonoidsare synthesized through phenylpropanoid and phenylpropanoid-acetate pathways and significantly contribute against adverse effect of abiotic and biotic stresses. Herein, we present the development and execution of a novel and expeditious ultra-performance liquid chromatographic-photodiode array (UPLC-PDA) method for qualitative and quantitative analysis of 28 phenolic compounds comprising of flavonoids, phenolic acids, aldehydes and alcohols. The method is able to separate phenolic compounds in just 17 min with the separation of isobaric species such as 3,4 dihydroxybenzoic acid and 3,5 dihydroxy benzoic acid; quercetin and taxifolin. Linear curves concentrations ranged from 6-18 µg/mL (3,5 dihydroxy benzoic acid), 4-12 µg/mL (catechin and salicylic acid) and 2-6 µg/mL for rest of the compounds and correlation coefficients were >0.994. The limit of detection (LOD) varied from 0.04-0.45 µg/mL. Cotton root samples were used to assess the method in terms of recovery efficiency (85-120%), precision (0.12-4.09%) and intermediate precision (0.32-4.0%).Phenolics and flavonoidsin root samples of healthy and diseased plants as well as leaf samples of healthy plants were successfully quantified using this novel method without an expensive Mass Spectrometer.

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Section: Linearity Loq and Lodsupporting

confidence: 93%

Simultaneous Estimation of Twenty Eight Phenolic Compounds by a Novel and Expeditious Method Developed on Quaternary Ultra-Performance Liquid Chromatography System with a Photodiode Array Detector

2019

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“…During vinification, only a portion of catechins and procyanidins is extracted from seeds and is diffused to wine [31]. The use of fluorescence detector has allowed increasing selectivity and sensitivity for the determination of the concentration of catechin and epicatechin in addition to other phenolic compounds [32]. Catechin, epicatechin, and procyanidin dimer (B2) were identified by fluorescence detector in wine samples (Fig.…”

Section: In Wine Samples; (1) Myricetin-3-glucoside; (2) Quercetin-3-mentioning

confidence: 99%

Antioxidant activity and polyphenol profile of Vranac red wines from Balkan region

Kostić

Pavlović

Micić

et al. 2016

Hem Ind

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The objective of the present study was to investigate the correlation between the radical--scavenging properties (measured by evaluating the quenching of the stable 2,2-diphenyl--1-picrylhydrazil radical) of Serbian, Macedonian and Montenegrin red wine Vranac of different geographical origins, and their contents of total phenolics, total flavonoids and polyphenol profile. All tested Vranac wines samples showed a high antioxidant activity ranging from 13.00 to 15.02 mmol/L, while the total polyphenolic content was between 3478.70 and 3935.19 mg/L. The predominant anthocyanin was malvidin-3-glucoside (179.04-281.31 mg/L), predominant flavonol was quercetin-3-glucoronide (5.88-11.78 mg/L), predominant flavan-3-ol was catechin (24.43-76.78 mg/L) and predominant hydroxycinnamic acid was t-caftaric acid (13.46-38.56 mg/L). Generally, red wines Vranac produced from Balkan regions are rich source of phenolics, which the evident antioxidant capacity showed.

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“…Liquid-liquid extraction and solid phase extraction are the methods most widely used to simplify chromatograms of wine samples (del Alamo et al, 2004;GarciaFalcon et al, 2007;Malovana, Montelongo, Perez, & RodriguezDelgado, 2001). Some authors inject the samples directly in HPLC without any preparation (Dias, Lovillo, Barroso, & David, 2010;Fang, Li, Pan, Food Research International 45 (2012) 145-154 & Huang, 2007;Lopez, Martinez, Del Valle, Orte, & Miro, 2001;. This controversy among different sample preparation makes necessary to develop a study of different alternatives.…”

Section: Introductionmentioning

confidence: 99%

Determination of phenolic composition and antioxidant capacity of native red wines by high performance liquid chromatography and spectrophotometric methods

Porgalı

Büyüktuncel

2012

Food Research International

109

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Optimization and validation of a method for the direct determination of catechin and epicatechin in red wines by HPLC/fluorescence

Cited by 38 publications

References 26 publications

Simultaneous Estimation of Twenty Eight Phenolic Compounds by a Novel and Expeditious Method Developed on Quaternary Ultra-Performance Liquid Chromatography System with a Photodiode Array Detector

Simultaneous Estimation of Twenty Eight Phenolic Compounds by a Novel and Expeditious Method Developed on Quaternary Ultra-Performance Liquid Chromatography System with a Photodiode Array Detector

Antioxidant activity and polyphenol profile of Vranac red wines from Balkan region

Determination of phenolic composition and antioxidant capacity of native red wines by high performance liquid chromatography and spectrophotometric methods

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