Optimization and Performance of Silica‐Based Media for Industrial‐Scale Antibody Purification

Abstract

A silica-based medium with optimized pore size, pore volume, and coupling density was prepared for protein A affinity chromatography, to be used for industrial purification of IgG. Performance and durability of the silica-based medium were studied during repeated use and compared with other protein A media available on the market. The silica-based medium provided high dynamic binding capacities for human polyclonal IgG in the superficial liquid velocity range from 150 to 720 cm h -1 because of a high mass transfer coefficient and high mechanical strength. The dynamic binding capacity and IgG recovery were high and varied only slightly during 50-150 cycles of repeated use. The release of protein A ligand was low.