Optimization and Modeling of Laccase Production by Trametes versicolor in a Bioreactor Using Statistical Experimental Design

Tavares, Ana P. M.; Coelho, Maria Alice Zarur; Agapito, M S M; Coutinho, João A. P.; Xavier, Ana M. R. B.

doi:10.1385/abab:134:3:233

Cited by 82 publications

(51 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The results of the present work will be the starting point for future approaches to biotechnological applications, such as biopulping and laccase production at higher level. Both systems are highly affected by agitation, as reported by other authors (Hess et al 2002;Mohorčič et al 2004;Tavares et al 2006). For C. versicolor f. antarcticus BAFC 266, fungal growth was highest at 25 ºC and pH 5.5 at the 10 th day of culture (p<0.05), whilst at higher incubation temperatures (29 °C and 33 °C) mycelium growth was favored at pH 4.5.…”

Section: Resultssupporting

confidence: 69%

Influence of Culture Conditions on Laccase Production, Growth, and Isoenzymes Patterns in Native White Rot Fungi from the Misiones Rainforest (Argentina)

et al. 2013

View full text Add to dashboard Cite

aMany biotechnological processes pursuing sustainability aim for effective, inexpensive, and environmentally friendly alternatives to replace conventional practices. Laccase-containing lignocellulolytic systems from white rot fungi have been shown to be an efficient enzymatic tool for ecofriendly biological treatments. One objective of the biotechnological enzymes production process is to find optimum growing and secretion conditions for a selected fungus. In this work, different fungi isolated from the Misiones rainforest (Coriolus versicolor f. antarcticus BAFC-266, Ganoderma applanatum strain F, Phlebia brevispora BAFC-633, and Pycnoporus sanguineus BAFC-2126) were incubated at different temperatures (25, 29, 33 °C) and pH values (3.5, 4.5, 5.5) under static conditions for 7, 10, and 14 days to evaluate their growing ability and laccase (Lac) production. Results revealed specific favorable conditions for growth and protein secretion depending on the fungus under consideration, making it necessary to adjust these parameters for each particular case. The combined effect of these cultivation parameters showed a marked influence on the secreted Lac activity by P. brevispora BAFC 633, with the highest activity (~ 240 U/l) at 29 ºC and pH 4.5 at the 10 th day of cultivation. The presence of Lac isoenzymes also depended on the pH, temperature, and time of cultivation for the different tested fungi.

show abstract

Section: Resultssupporting

confidence: 69%

Influence of Culture Conditions on Laccase Production, Growth, and Isoenzymes Patterns in Native White Rot Fungi from the Misiones Rainforest (Argentina)

et al. 2013

View full text Add to dashboard Cite

show abstract

“…The maintenance and growth parameters were lumped to a single parameter c in the growth phase or to c d in the death phase, respectively. Previously, also Tavares et al [51] based their growth kinetics parameters on two phases of growth when modeling cell growth, substrate consumption and laccase production of Trametes versicolor in SmC. Similarly to us, they described the laccase production using the LuedekingPiret model.…”

Section: Discussionmentioning

confidence: 90%

Production of manganese peroxidase and laccase in a solid-state bioreactor and modeling of enzyme production kinetics

Moilanen¹,

Winquist²,

Mattila³

et al. 2014

Bioprocess Biosyst Eng

View full text Add to dashboard Cite

Lignin-modifying enzymes have various promising applications such as biobleaching, biopulping, the functionalization of lignocellulosic materials, the modification of wood fibers, the remediation of contaminated soil and effluents, as well as improvement of the enzymatic hydrolysis of lignocellulosic substrates. In this study, the production of laccase and manganese peroxidase (MnP) in solid-state cultivation was examined. Oat husks were used as an inexpensive substrate for the white-rot fungus Cerrena unicolor PM170798 (FBCC 387). The addition of a fines fraction (consisting of oat flour and finely ground husks) enhanced MnP production fivefold and laccase production almost threefold. The enzyme production was studied first on a 100 g scale, and the cultivation experiments were then repeated at a larger laboratory-scale (4 kg) in a solid-state bioreactor. High enzyme activity levels were obtained (MnP: 340 nkat g(-1) DM, laccase: 470 nkat g(-1) DM). In addition, the correlation between the CO2 evolution rate and enzyme production was mathematically modeled from the bioreactor experimental data. The model parameters could be used to predict enzyme production.

show abstract

“…When final optimization was done using central composite design [24], laccase production has also been increased by 28.3% under submerged fermentation by Taguchi DOE methodology by Pleurotus ostreatus [25]. Optimization and modeling of laccase production by Trametes versicolor in a bioreactor using statistical experimental design resulted in 11,403 U/l laccase activity [26]. Response surface methodology was also applied for the decolorization of the azo dye reactive black 5 (RB-5) using purified laccase from Pleurotus sajorcaju.…”

Section: Discussionmentioning

confidence: 99%

Optimization of media components for laccase production by litter dwelling fungal isolate Fusarium incarnatum LD‐3

Chhaya

Gupte

2010

J. Basic Microbiol.

View full text Add to dashboard Cite

Laccase production by solid state fermentation (SSF) using an indigenously isolated litter dwelling fungus Fusarium incarnatum LD-3 was optimized. Fourteen medium components were screened by the initial screening method of Plackett-Burman. Each of the components was screened on the basis of 'p' (probability value) which was above 95% confidence level. Ortho-dianisidine, thiamine HCl and CuSO(4) . 5 H(2)O were identified as significant components for laccase production. The Central Composite Design response surface methodology was then applied to further optimize the laccase production. The optimal concentration of these three medium components for higher laccase production were (g/l): CuSO(4) . 5 H(2)O, 0.01; thiamine HCl, 0.0136 and ortho-dianisidine, 0.388 mM served as an inducer. Wheat straw, 5.0 g was used as a solid substrate. Using this statistical optimization method the laccase production was found to increase from 40 U/g to 650 U/g of wheat straw, which was sixteen times higher than non optimized medium. This is the first report on statistical optimization of laccase production from Fusarium incarnatum LD-3.

show abstract

Optimization and Modeling of Laccase Production by Trametes versicolor in a Bioreactor Using Statistical Experimental Design

Cited by 82 publications

References 38 publications

Influence of Culture Conditions on Laccase Production, Growth, and Isoenzymes Patterns in Native White Rot Fungi from the Misiones Rainforest (Argentina)

Influence of Culture Conditions on Laccase Production, Growth, and Isoenzymes Patterns in Native White Rot Fungi from the Misiones Rainforest (Argentina)

Production of manganese peroxidase and laccase in a solid-state bioreactor and modeling of enzyme production kinetics

Optimization of media components for laccase production by litter dwelling fungal isolate Fusarium incarnatum LD‐3

Contact Info

Product

Resources

About