Optimization and evaluation of the performance of arrangements for UV detection in high-resolution separations using fused-silica capillaries

Bruin, Gerard; Stegeman, G.; Asten, Arian C. van; Xu, X.; Kraak, J.C.; Poppe, H.

doi:10.1016/0021-9673(91)80068-r

Cited by 83 publications

(49 citation statements)

References 23 publications

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“…Consequently, limits of detection are about 25-50 times lower compared to on-column detection. For instance, the limit of detection for uracil with on-column detection was 3.1.10 -6 mol 1-], and with the longitudinal flow cell it was equal to 9.8" 10 -8 mol 1-t [ 138]. The contribution of longitudinal flow cells to extracolumn band broadening, i.e.…”

Section: Uv Absorbance Detectionmentioning

confidence: 97%

“…Limits of detection reported for on-column detection are typically around 1-5.10 6 mol 1-J for compounds such as uracil, cytosine and thymine, with a 100 I~m I.D. flow cell [138]. Despite the extremely small path lengths, detection is feasible at the sub-ng level.…”

Section: Uv Absorbance Detectionmentioning

confidence: 99%

“…A more successful approach towards improvement of the detection sensitivity with UV absorbance detection in microcolumn LC was the introduction of longitudinal flow cells with an optical path length up to 3-8 mm [141]. This type of flow cell and on-column flow cells have been studied extensively with respect to sensitivity, linear dynamic range, and its contribution to extracolumn band broadening and noise [ 138,142]. The sensitivity of the longitudinal type of cells is normally 50-100 times higher compared to on-column detection.…”

Section: Uv Absorbance Detectionmentioning

confidence: 99%

See 2 more Smart Citations

Microcolumn liquid chromatography: instrumentation, detection and applications

Vissers¹,

Claessens

Cramers

1997

Journal of Chromatography A

208

101

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This review discusses many different aspects of microcolumn liquid chromatography (LC) and reflects the areas of microcolumn LC research interest over the past decades. A brief theoretical discussion on a number of major issues, such as column characterisation, chromatographic dilution effects and extracolumn band broadening in microcolumn LC is given. Recent progress in column technology and the demands and developments of instrumentation and accessories for microcolumn LC are also reviewed. Besides that, the developments in a large number of established and also more recent detection techniques for microcolumn LC are also discussed. The potential of hyphenation of microcolumn LC with other techniques, more particularly of multidimensional chromatography and microcolumn LC coupled to mass spectrometry is reviewed. Finally, the perspective of microcolumn LC separation methods is stressed by a number of relevant applications.

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Section: Uv Absorbance Detectionmentioning

confidence: 97%

Section: Uv Absorbance Detectionmentioning

confidence: 99%

Section: Uv Absorbance Detectionmentioning

confidence: 99%

See 1 more Smart Citation

Microcolumn liquid chromatography: instrumentation, detection and applications

Vissers¹,

Claessens

Cramers

1997

Journal of Chromatography A

208

101

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“…The pressure was monitored with a digital pressure sensor (Type 4041A200; Kistler, Winterthur, Switzerland) combined with a current amplifier (Type 4061; Kistler) and a recorder (Model BDlll; Kipp & Zonen, Delft, Netherlands). Two on-column detection systems were employed, a UV absorbance detector (Kratos Model 757) using a laboratory-built on-column detection cell with adjustable slit [6] (the detector rise time was set to 0.1 s) and a heliumcadmium laser ()rex = 325 nm) (Model 356 XM; Omnichrome, Chino, CA, USA) used as a light source for laser-induced fluorescence detection (LIF) with emission wavelength set at 380 nm. The fluorescence yield was measured with a…”

Section: Apparatusmentioning

confidence: 99%

Performance of an ethoxyethylacrylate stationary phase for open-tubular liquid chromatography

Swart

Kraak

Poppe

1995

Journal of Chromatography A

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Disclaimer/Complaints regulationsIf you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library: http://uba.uva.nl/en/contact, or a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible. AbstractFused-silica capillaries of I.D. ca 11.4 tzm were coated with polyacrylate layers. The layers consisted of copolymers of silicone acrylate and ethoxyethylacrylate which were polymerized in situ by UV irradiation. Capillaries with phase ratios up to 0.9 were prepared. This results in very high mass loadabilities so that solute amounts suitable for UV detection can be injected without overloading the stationary phase. This is illustrated by the separation of various mixtures containing phenolic and aromatic compounds. The retention behaviour and the kinetic performance were studied with both UV and laser-induced fluorescence detection. On a 5-m column more than 10 6 plates could be generated for anthracene. Further, the possibility of on-column preconcentration and the effect of temperature on the kinetic performance were investigated on this stationary phase.

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“…On-column UV-detection is most commonly used in CZE. One of the main restrictions is the high detection limit, in the 10-5-10-6 mol/1 range [3], as a result of the short available light path, the inner diameter of the capillary. Capillaries with diameters of 10-100 gm I.D.…”

Section: Introductionmentioning

confidence: 99%

Correlation capillary zone electrophoresis, a novel technique to decrease detection limits

et al. 1995

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General rightsIt is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), other than for strictly personal, individual use, unless the work is under an open content license (like Creative Commons). Disclaimer/Complaints regulationsIf you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library: http://uba.uva.nl/en/contact, or a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible. SummaryThe application of multiple injections and correlation techniques in capillary zone electrophoresis, a method known for its high detection limits, is described. An experimental electrokinetic injection device, particulary suited for fast and reproducible multiple injections is used in combination with a conventional CZE system. The results show a considerable reduction of the detection limit, in agreement with the experience in the already fully developed correlation chromatographic methods, and in agreement with the theoretical predicted values.

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Optimization and evaluation of the performance of arrangements for UV detection in high-resolution separations using fused-silica capillaries

Cited by 83 publications

References 23 publications

Microcolumn liquid chromatography: instrumentation, detection and applications

Microcolumn liquid chromatography: instrumentation, detection and applications

Performance of an ethoxyethylacrylate stationary phase for open-tubular liquid chromatography

Correlation capillary zone electrophoresis, a novel technique to decrease detection limits

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