Abstract:Fabrication and characterization of chitosan/poly(vinyl alcohol) electrospun nanofibers for adsorption of phenol from water were investigated. The effects of voltage (15–30 kV), solution injection flow rate (0.5–1.5 ml/hr), distance of needle and collector (10–20 cm) and chitosan/poly(vinyl alcohol) ratio (25/75, 50/50, 75/25) were studied to obtain the optimum electrospinning conditions for the maximum adsorption capacity of phenol. Central composite design (CCD) was used to investigate and optimize the proce… Show more
“…As a result, increased surface charge density results in nanofibers with smaller diameters and increases the drag force imposed on the polymer jet flow. 45,46 Figure 5.Main effects of the defined variables on CS/PEO fiber diameter a) concentration, b) voltage, c) distance, and d) angle.…”
Section: Resultsmentioning
confidence: 99%
“…result, increased surface charge density results in nanofibers with smaller diameters and increases the drag force imposed on the polymer jet flow. 45,46 The obtained term coefficient between electrospinning parameters and response variables indicated that the mean diameter of nanofibers significantly decreased with an increase in the applied voltage (r = À 0.267, p value <.0001). In Figure 5(b), The inverse relationship between the applied voltage and the average diameter of the fiber is explained by the fact that increasing the applied voltage increases the strength of the electric field and electrostatic stretching force, which results in an accelerated jet and thinner nanofibers.…”
Section: Investigation Of the Effect Of Processing Variables On The R...mentioning
The present study is an attempt to optimize the electrospinning parameters to fabricate chitosan/polyethylene oxide hybrid nanofiber composite with minimum diameter and coefficient of variation (homogeneity) by using response surface methodology (RSM). Based on central composite design (CCD), four factors (the chitosan/PEO ratio, the applied voltage, the needle-to-collector distance, and the spinning angle) was applied to evaluate the individual and combined effects of the parameters on the average diameter of nanofibers. Chitosan (CH) was blended with Polyethylene oxide (PEO) at weight ratios of 1:1, 2:1, 3:1, 4:1, and 5:1. As the composition ratio of chitosan was reduced, the viscosity of the chitosan/PEO blend solution also dropped. The RSM model identified the following conditions as necessary to produce the desired and most uniform CS/PEO fiber diameter: a CS/PEO blend ratio of 2:1 (w/w), a voltage of 25 kV, a distance of 20 cm, and a spinning angle of 45°. The average nanofiber diameter and homogeneity under these conditions were calculated to be 73 nm and 20.5%, respectively, compared to the predicted values of 71 nm and 15.58%, respectively.
“…As a result, increased surface charge density results in nanofibers with smaller diameters and increases the drag force imposed on the polymer jet flow. 45,46 Figure 5.Main effects of the defined variables on CS/PEO fiber diameter a) concentration, b) voltage, c) distance, and d) angle.…”
Section: Resultsmentioning
confidence: 99%
“…result, increased surface charge density results in nanofibers with smaller diameters and increases the drag force imposed on the polymer jet flow. 45,46 The obtained term coefficient between electrospinning parameters and response variables indicated that the mean diameter of nanofibers significantly decreased with an increase in the applied voltage (r = À 0.267, p value <.0001). In Figure 5(b), The inverse relationship between the applied voltage and the average diameter of the fiber is explained by the fact that increasing the applied voltage increases the strength of the electric field and electrostatic stretching force, which results in an accelerated jet and thinner nanofibers.…”
Section: Investigation Of the Effect Of Processing Variables On The R...mentioning
The present study is an attempt to optimize the electrospinning parameters to fabricate chitosan/polyethylene oxide hybrid nanofiber composite with minimum diameter and coefficient of variation (homogeneity) by using response surface methodology (RSM). Based on central composite design (CCD), four factors (the chitosan/PEO ratio, the applied voltage, the needle-to-collector distance, and the spinning angle) was applied to evaluate the individual and combined effects of the parameters on the average diameter of nanofibers. Chitosan (CH) was blended with Polyethylene oxide (PEO) at weight ratios of 1:1, 2:1, 3:1, 4:1, and 5:1. As the composition ratio of chitosan was reduced, the viscosity of the chitosan/PEO blend solution also dropped. The RSM model identified the following conditions as necessary to produce the desired and most uniform CS/PEO fiber diameter: a CS/PEO blend ratio of 2:1 (w/w), a voltage of 25 kV, a distance of 20 cm, and a spinning angle of 45°. The average nanofiber diameter and homogeneity under these conditions were calculated to be 73 nm and 20.5%, respectively, compared to the predicted values of 71 nm and 15.58%, respectively.
“…In electrospinning process, the entrapment of cells in nanofibers can be achieved by direct electrospinning of cells with other components such as polymers. Commonly, water soluble polymers are used to entrap cells due to their properties such as good fiber forming characteristic, non-toxic, able to retain high bioavailability of the cells and will undergo solvent evaporation during the electrospinning process which can help in extending the shelf life of cells [39,40].On top of that, these polymers show relatively high degradation in aqueous environment which is desirable in applications that need quick release of cells [21]. Therefore, in most cases, water soluble polymers have been used to dissolve the cells so that they can form homogenous solution with the cells.…”
Section: Entrapment Principle Of Cells In Electrospun Nanofibersmentioning
Nanotechnology is a growing technology that has been recognized as vital and scientific with bioprocess development especially in dealing with usage of free cells. Limitations such as low bioavailability, storage instability and low substrate inhibition bound the application of free cells in various field thus leading researchers into focusing more on free cells immobilization system. With the increasing knowledge in nanomaterials fabrication techniques, the immobilization of free cells through entrapment approach in highly porous and the high surface area of nanofibers is becoming an interesting subject to be highlighted. The production of free cells entrapped in nanofibers via electrospinning in terms of quality and quantity is highly affected by the electrospinning operating parameters including solution formulation, ambient conditions, operating conditions of electrospinning machine and types of materials used. Hence, this review paper will provide an overview of the operating conditions involved in electrospinning of cells through entrapment process which affect the characteristics of the electrospun nanofibers produced and the viability or growth of cells when entrapped in the electrospun nanofibers mats.
“…Linear or square polynomial functions are employed to describe the system studied and to explore (modeling and displacing) the optimal experimental conditions. [7] Regression analysis is a modeling technique, which determines the relationship between a dependent (target) and independent variables (predictor). Using the regression methods, the experimental data thus obtained were utilized to build mathematical models which analyze the optimal conditions for the process.…”
Aim: Recombinant human asparaginase (rhASP) from Escherichia coli is an important therapeutic enzyme used in the treatment of malignant cancers. Due to such a pivotal role, rhASP production in E. coli has drawn great attention of the biopharmaceutical market commercially. The present work aims at the optimization of fermentation medium for the production of rhASP in E. coli. Materials and Methods: The rhASP yield is optimized using sequential optimization designs comprising one variable at a time, Taguchi design, and central composite designs (CCDs). Taguchi design is used to select the effective variables such as soytone, sodium pyruvate, trace element solution, vitamin solution, and yeast extract, which are further optimized by CCD under response surface methodology. Results and Discussion: The CCD design developed a quadratic model with high adequacy for the prediction of rhASP yield with a statistically significant response (R 2 = 97.49% and P < 0.0001) toward the variables. Conclusion: The CCD results showed that the maximum rhASP yield of 38.4387 µg/mL was achieved with the optimized concentrations of media components comprising 9.0 g/L of soytone, 7.5 g/L of sodium pyruvate, 12.5 mL/L of trace element solution, 12.5 mL/L of vitamin solution, and 9.0 g/L of yeast extract.
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