Optimization and characterization of Alginic acid synthesized from a novel strain of Pseudomonas stutzeri

Meena, S.; M, Vidya Kalaivani; Tripathi, Abhishek Dutt; Ts, Ramyaa Lakshmi

doi:10.1016/j.btre.2020.e00517

Cited by 13 publications

(3 citation statements)

References 54 publications

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“…These broad bands at 4.7 ppm, 3.4 ppm, 3.08 ppm, and 5.1 ppm are confirmed to be strongly predictive of the presence of the G and M blocks of alginate in our documentary. The resonances were in good agreement with those published in the literature [ 63 , 65 ]. The 13C NMR analysis (Fig.…”

Section: Resultssupporting

confidence: 90%

“…Alginate G5-H2 moieties of L-guluronic acid in homopolymeric blocks are attributed to the detected signal between 4.2 and 5.1 ppm [ 62 ]. A resonance below 3.2 ppm, a property of proteins and lipids, as well as acetyl and succinyl groups, was also detected in the spectrum fraction of cyanobacterial alginate [ 63 , 64 ]. These broad bands at 4.7 ppm, 3.4 ppm, 3.08 ppm, and 5.1 ppm are confirmed to be strongly predictive of the presence of the G and M blocks of alginate in our documentary.…”

Section: Resultsmentioning

confidence: 99%

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Identification and statistical optimization of a novel alginate polymer extracted from newly isolated Synechocystis algini MNE ON864447 with antibacterial activity

Saad,

Sidkey,

El-Fakharany

2023

Microb Cell Fact

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Cyanobacteria are a potential source of promising secondary metabolites with different biological activities, including antibacterial, antiviral, antifungal, antiprotozoal, and anticancer activities. To combat the emergence of antibiotic resistance, there is an urgent requirement for new drugs, and cyanobacteria metabolites can constitute alternative new antibacterial medication. The chemical complexity of their exopolysaccharides indicates that they have the potential to be bioactive molecules with many biological activities. The present study aimed to produce and optimise a novel alginate polymer from a newly isolated cyanobacterium, S. algini MNE ON864447, in addition to its promising antibacterial activity. We successfully isolated a new cyanobacterium strain, S. algini MNE ON864447 from the Nile River, which produces alginate as an extracellular polymeric substance. The isolated cyanobacterial alginate was identified using a set of tests, including FTIR, TLC, HPLC, GC–MS, and 1H NMR. Plackett–Burman statistical design showed that working volume (X1), the incubation period (X2), and inoculum size (X3) are the most significant variables affecting the production of alginate. The highest alginate production (3.57 g/L) was obtained using 4% inoculum size in 400 mL medium/L conical flask after 20 days of the incubation period. The extracted alginate showed potent antibacterial activity against both Gram-negative and Gram-positive bacteria and Streptococcus mutants (NCTC10449) are the most sensitive tested pathogen for purified cyanobacterial alginate with inhibition zone diameters of 34 ± 0.1 mm at 10 mg/mL of purified alginate while Vibro cholera (NCTC 8021) the lowest sensitive one and showed inhibition zone diameters of 22.5 ± 0.05 mm at the same cyanobacterial alginate concentration. This antibacterial activity is a critical step in the development of antibacterial drugs and presents a new challenge to fight against multi-resistant bacteria.

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Section: Resultssupporting

confidence: 90%

Section: Resultsmentioning

confidence: 99%

Identification and statistical optimization of a novel alginate polymer extracted from newly isolated Synechocystis algini MNE ON864447 with antibacterial activity

Saad,

Sidkey,

El-Fakharany

2023

Microb Cell Fact

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“…Streptococcus equi , S. zooepidemicus , S. pyogenes , Pasteurella multocida , and Cryptococcus neoformans are natural bacteria that produce hyaluronic acid (Sze et al 2016 ). Alginate is linear HePS with solubility in water (S et al 2020 ). Its subunits consist of d -manopyranuronic acid and l -guluronic acid (Sun and Zhang 2021 ).…”

Section: Classification and Properties Of Microbial Epssmentioning

confidence: 99%

Recent advances in the biological activities of microbial exopolysaccharides

Salimi

Farrokh

2023

World J Microbiol Biotechnol

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Microbial exopolysaccharides (EPSs) are valuable extracellular macromolecules secreted as capsules or slime layers. Various microorganisms, including bacteria, yeasts, fungi, and algae have been studied for their ability to produce EPSs. Microbial EPSs exist as homopolysaccharides or heteropolysaccharides with various properties such as different monosaccharide compositions, structural conformation, molecular weight, and functional groups. They are cost-effective alternatives to plant and animal-derived polysaccharides because the microbial cells produced them in large quantities by biotechnological processes using low-cost substrates such as industrial wastes in a short time. Microbial EPSs are safe, biodegradable, and compatible polymers. They have extensive bioactivities, including antibacterial, antifungal, antiviral, antioxidant, antitumor, antidiabetic, antiulcer, anticoagulant, antiaging, immunomodulatory, wound healing, and cholesterol-lowering activities. Microbial EPSs owing to biological activities, special biochemical structures, and attractive physicochemical properties find plenty of potential applications in various industries. The enhancement of the production of EPSs and improving their properties can be provided by genetic engineering methods. The current review aims to provide a comprehensive examination of the therapeutic activities of microbial EPSs in infectious diseases and metabolic disorders, with a focus on the mechanisms involved. Also, the effect of the physicochemical characteristics of EPSs on these bioactivities was discussed to reveal the structure-activity relationship.

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Composite of Natural Polymers and Their Adsorbent Properties on the Dyes and Heavy Metal Ions

Kayan

2021

J Polym Environ

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Optimization and characterization of Alginic acid synthesized from a novel strain of Pseudomonas stutzeri

Abstract: Graphical abstract

Cited by 13 publications

References 54 publications

Identification and statistical optimization of a novel alginate polymer extracted from newly isolated Synechocystis algini MNE ON864447 with antibacterial activity

Identification and statistical optimization of a novel alginate polymer extracted from newly isolated Synechocystis algini MNE ON864447 with antibacterial activity

Recent advances in the biological activities of microbial exopolysaccharides

Composite of Natural Polymers and Their Adsorbent Properties on the Dyes and Heavy Metal Ions

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