Optimization and application of a high-resolution melting protocol in the characterization of avian infectious laryngotracheitis virus

Rojas, María; König, Guido; Vagnozzi, Ariel; Vera, Federico; Scolaro, Luis Alberto; Craig, María Isabel

doi:10.1016/j.ram.2020.04.008

Cited by 2 publications

(3 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our optimization of SSR-HRM indicated that a low annealing temperature (56 • C, Groups 1, 4, 7) resulted in nonspecific amplification which generated incorrect SSR genotyping. The DNA amounts of different HRM protocols ranged from 20 to 50 ng in the 10 µL mixture [16,22,26,29]. The excess DNA of R. rugosa (100 ng, Groups 7, 8, 9) generated a weaker fluorescence signal and decreased both specificity and reproducibility.…”

Section: Discussionmentioning

confidence: 99%

“…Biomolecules 2023, 13, x FOR PEER REVIEW 5 of 9 loci included dimer to hexamer motifs except the trimer. The SSR-HRM analysis of the 19 cultivars and the wild R. rugosa produced three (SSR4), four (SSR11, 13, 28), five (29,31), six (SSR9, 12) and nine (SSR19) genotypes, respectively (Table S1). Genotypes of the most SSRs included at least two cultivars (e.g., SSR9 or SSR12, Figure 2) except for SSR19 (Figure 2E).…”

Section: Ssr Fingerprint Constructionmentioning

confidence: 99%

See 1 more Smart Citation

Simple Sequence Repeat Fingerprint Identification of Essential-Oil-Bearing Rosa rugosa via High-Resolution Melting (HRM) Analysis

Xu,

Wang,

Feng

et al. 2023

Biomolecules

View full text Add to dashboard Cite

Oil-bearing Rosa rugosa are popular in the essential oil and perfume markets. The similar botanical characteristics between high-oil-yield or low-oil-yield cultivars are confusing and it is hard for farmers or breeders to identify the high-oil-yield cultivar by phenotype difference. High-resolution melting (HRM) analysis of simple sequence repeats (SSRs) can construct accurate DNA fingerprints quickly, which was shown to be effective for identification of closely related cultivars of R. rugosa. Optimization of HRM-SSR indicated that the 10 µL HRM reaction mixture containing 20 ng of genomic DNA of R. rugosa and 0.75 µL of 10 µmol/L of each primer with an annealing temperature of 64 °C was a robust SSR genotyping protocol. Using this protocol, 9 polymorphic SSR markers with 3–9 genotypes among the 19 R. rugosa cultivars were identified. The top three polymorphic makers SSR9, SSR12 and SSR19 constructed a fingerprint of all cultivars, and the rare insertion in the flanking sequences of the repeat motif of SSR19 generated three characteristic genotypes of three high-oil-yield cultivars. These results may be economical and practical for the identification of high-oil-yield R. rugosa and be helpful for the selection and breeding of oil-bearing roses.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Ssr Fingerprint Constructionmentioning

confidence: 99%

Simple Sequence Repeat Fingerprint Identification of Essential-Oil-Bearing Rosa rugosa via High-Resolution Melting (HRM) Analysis

Xu,

Wang,

Feng

et al. 2023

Biomolecules

View full text Add to dashboard Cite

show abstract

“…That has a worldwide distribution and can cause economic losses during repeat outbreaks. Caused by herpes virus with order Herpesvirales, family Herpesviridae, subfamily alphaherpesvirinae, genus Iltovirus, species Gallid herpesvirus 1 (GaHV-1) (Rojas et al, 2021), Chickens are the primary natural host, and all ages are susceptible but birds older than three weeks are more susceptible (Dufour-Zavala, 2008). Pheasant and peafowl are susceptible to ILT virus (Abdul-Aziz & Barnes, 2018).…”

Section: Introductionmentioning

confidence: 99%

Molecular detection and isolation of infectious laryngotracheitis virus (ILTV) in layer farms of Waset province, Iraq

Hussein

Abdullah

2022

ijhs

View full text Add to dashboard Cite

Infectious Laryngotracheitis (ILT) is an acute highly contagious respiratory disease of chickens. It has significant economic importance due to mortalities and the decrease in egg production. In this study, twenty nine samples from different layer farms were collected from the outbreaks that occurred in waset province, Iraq, at the period from November 2021 till March 2022 to detect ILTV by molecular test through real time polymerase chain reaction assay (RT-PCR) as well as isolation on embryonated chicken eggs through chorioallantoic membrane (CAM) route. The clinical signs examination of infected birds revealed extend head with expectoration of bloody mucous, gasping with weeping eye (watery eye). Gross pathological lesions examination revealed catarrhal, caseated, fibrinonecrotic, hemorrhagic tracheitis. The RT-PCR revealed amplification specific for glycoprotein G gene of ILT virus. Preparation and Inoculation of tissue samples which giving positive results with RT-PCR on embryonated chicken eggs (ECE) appeared as white pock lesions on the inoculated CAM from the second passage.

show abstract

Optimization and application of a high-resolution melting protocol in the characterization of avian infectious laryngotracheitis virus

Cited by 2 publications

References 26 publications

Simple Sequence Repeat Fingerprint Identification of Essential-Oil-Bearing Rosa rugosa via High-Resolution Melting (HRM) Analysis

Simple Sequence Repeat Fingerprint Identification of Essential-Oil-Bearing Rosa rugosa via High-Resolution Melting (HRM) Analysis

Molecular detection and isolation of infectious laryngotracheitis virus (ILTV) in layer farms of Waset province, Iraq

Contact Info

Product

Resources

About