Infectious Laryngotracheitis (ILT) is an acute highly contagious respiratory disease of chickens. It has significant economic importance due to mortalities and the decrease in egg production. In this study, twenty nine samples from different layer farms were collected from the outbreaks that occurred in waset province, Iraq, at the period from November 2021 till March 2022 to detect ILTV by molecular test through real time polymerase chain reaction assay (RT-PCR) as well as isolation on embryonated chicken eggs through chorioallantoic membrane (CAM) route. The clinical signs examination of infected birds revealed extend head with expectoration of bloody mucous, gasping with weeping eye (watery eye). Gross pathological lesions examination revealed catarrhal, caseated, fibrinonecrotic, hemorrhagic tracheitis. The RT-PCR revealed amplification specific for glycoprotein G gene of ILT virus. Preparation and Inoculation of tissue samples which giving positive results with RT-PCR on embryonated chicken eggs (ECE) appeared as white pock lesions on the inoculated CAM from the second passage.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.