Optimising effective parameters to improve performance quality in lateral flow immunoassay for detection of <i>PBP2a</i> in methicillin-resistant <i>Staphylococcus aureus</i> (MRSA)

Amini, Mohsen; Pourmand, Mohammad Reza; Faridi‐Majidi, Reza; Heiat, Mohammad; Nezhady, Mohammad Ali Mohammad; Safari, Mojtaba; Noorbakhsh, Farshid; Baharifar, Hadi

doi:10.1080/17458080.2020.1775197

Cited by 17 publications

(16 citation statements)

References 38 publications

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“…Sugar (such as sucrose) is the key component in the conjugate pad treat buffer to preserve the biological activity and function of the detective antibody by forming a layer at the surface of AuNP-antibody conjugate to keep the structure and the binding site 35,36 . Moreover, sucrose is believed to have a positive effect on the flow of the analyte because the sucrose layer dissolves immediately and works as a carrier of the detector to enter the fluid stream 36,37 . The effect of Tween 20 was firstly detected by testing the performance of the solution with Tween 20 concentrated from 1 to 7% (v/v).…”

Section: Resultsmentioning

confidence: 99%

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Quantitative brain-derived neurotrophic factor lateral flow assay for point-of-care detection of glaucoma

Jiang

et al. 2022

Lab Chip

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Section: Resultsmentioning

confidence: 99%

“…To fabricate the LFA strip, pretreated sample pad, conjugate pad and absorbance pad were assembled in the backing card with 2 mm overlap (Figure 4a) 37 . A red test line and a red control line were the positive results and only one line at the control area represented the negative result (Figure 4a).…”

Section: Resultsmentioning

confidence: 99%

Quantitative brain-derived neurotrophic factor lateral flow assay for point-of-care detection of glaucoma

Jiang

et al. 2022

Lab Chip

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“…Maldonado and co-authors developed a nanophotonic biosensor which uses a bimodal waveguide interferometer to differentiate MRSA from MSSA, based on PBP2a protein detection [ 136 ]. A last example of a phenotypic biosensor, based on a lateral flow immunoassay that detects the PBP2a, was developed by Amini and coauthors [ 137 ].…”

Section: Emerging Methods For Antimicrobial Resistance Detection In S Aureusmentioning

confidence: 99%

Recent Developments in Phenotypic and Molecular Diagnostic Methods for Antimicrobial Resistance Detection in Staphylococcus aureus: A Narrative Review

Sanchini¹

2022

Diagnostics

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Staphylococcus aureus is an opportunistic pathogen responsible for a wide range of infections in humans, such as skin and soft tissue infections, pneumonia, food poisoning or sepsis. Historically, S. aureus was able to rapidly adapt to anti-staphylococcal antibiotics and become resistant to several classes of antibiotics. Today, methicillin-resistant S. aureus (MRSA) is a multidrug-resistant pathogen and is one of the most common bacteria responsible for hospital-acquired infections and outbreaks, in community settings as well. The rapid and accurate diagnosis of antimicrobial resistance in S. aureus is crucial to the early initiation of directed antibiotic therapy and to improve clinical outcomes for patients. In this narrative review, I provide an overview of recent phenotypic and molecular diagnostic methods for antimicrobial resistance detection in S. aureus, with a particular focus on MRSA detection. I consider methods for resistance detection in both clinical samples and isolated S. aureus cultures, along with a brief discussion of the advantages and the challenges of implementing such methods in routine diagnostics.

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“…It should be noted that the pH value equal to 9.0 being chosen for IgG immobilization accords to common recommendations for preparation of GNP-IgG conjugates for analytical purposes [45]. Early and recent studies [22,[46][47][48] repeatedly confirm that this pH provides reaching both colloidal stability and high antigen-binding efficiency of the obtained products. The impact of pH to composition of conjugates was indicated earlier [32], and the technique for conjugates characterization presented in this paper may be used to evaluate this impact as well as impact of other parameters of the reaction media and properties of interacting molecules.…”

Section: Calculation Of the Numbers Of Immunoglobulin G Sorbed On Gnpsmentioning

confidence: 99%

Retention of Activity by Antibodies Immobilized on Gold Nanoparticles of Different Sizes: Fluorometric Method of Determination and Comparative Evaluation

et al. 2021

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Antibody–nanoparticle conjugates are widely used analytical reagents. An informative parameter reflecting the conjugates’ properties is the number of antibodies per nanoparticle that retain their antigen-binding ability. Estimation of this parameter is characterized by a lack of simple, reproducible methods. The proposed method is based on the registration of fluorescence of tryptophan residues contained in proteins and combines sequential measurements of first the immobilized antibody number and then the bound protein antigen number. Requirements for the measurement procedure have been determined to ensure reliable and accurate results. Using the developed technique, preparations of spherical gold nanoparticles obtained by the most common method of citrate reduction of gold salts (the Turkevich–Frens method) and varying in average diameter from 15 to 55 nm have been characterized. It was shown that the number of antibodies (immunoglobulins G) bound by one nanoparticle ranged from 30 to 194 during adsorptive unoriented monolayer immobilization. C-reactive protein was considered as the model antigen. The percentage of antibody valences that retained their antigen-binding properties in the conjugate increased from 17 to 34% with an increase in the diameter of gold nanoparticles. The proposed method and the results of the study provide tools to assess the capabilities of the preparations of gold nanoparticles and their conjugates as well as the expediency of seeking the best techniques for various practical purposes.

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Optimising effective parameters to improve performance quality in lateral flow immunoassay for detection of PBP2a in methicillin-resistant Staphylococcus aureus (MRSA)

Cited by 17 publications

References 38 publications

Quantitative brain-derived neurotrophic factor lateral flow assay for point-of-care detection of glaucoma

Quantitative brain-derived neurotrophic factor lateral flow assay for point-of-care detection of glaucoma

Recent Developments in Phenotypic and Molecular Diagnostic Methods for Antimicrobial Resistance Detection in Staphylococcus aureus: A Narrative Review

Retention of Activity by Antibodies Immobilized on Gold Nanoparticles of Different Sizes: Fluorometric Method of Determination and Comparative Evaluation

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