The main objective of this paper was to examine the effect of the increase of organic loading rates (OLRs) (by reducing solid retention time, SRT, from 20 d to 5 d) in single-phase mesophilic anaerobic co-digestion of the sewage sludge with glycerine (1% v/v). Experimentally, it was confirmed that anaerobic co-digestion of these biowastes in steady-state conditions can achieve 85±5% of volatile fatty acid (VFA) reduction at SRTs between 20 and 9 d, with a methane production around 0.8 l CH4/l/d. Decreases in the SRT not only allow maintaining the sludge stability and the biogas production, but it also implies an increase in the waste that could be treated and lower cost. Therefore, mesophilic anaerobic co-digestion of sewage sludge and glycerin at SRT lower than 20 d is possible and preferable, due to it is more economical and environmental friendly.