Optimisation of Cellulase Production by Penicillium funiculosum in a Stirred Tank Bioreactor Using Multivariate Response Surface Analysis

Abstract

Increasing interest in the production of second-generation ethanol necessitates the low-cost production of enzymes from the cellulolytic complex (endoglucanases, exoglucanases, and β-glucosidases), which act synergistically in cellulose breakdown. The present work aimed to optimise a bioprocess to produce these biocatalysts from the fungus Penicillium funiculosum ATCC11797. A statistical full factorial design (FFD) was employed to determine the optimal conditions for cellulase production. The optimal composition of culture media using Avicel (10 g·L−1) as carbon source was determined to include urea (1.2 g·L−1), yeast extract (1.0 g·L−1), KH2PO4 (6.0 g·L−1), and MgSO4 ·7H2O (1.2 g·L−1). The growth process was performed in batches in a bioreactor. Using a different FFD strategy, the optimised bioreactor operational conditions of an agitation speed of 220 rpm and aeration rate of 0.6 vvm allowed the obtainment of an enzyme pool with activities of 508 U·L−1 for FPase, 9,204 U·L−1 for endoglucanase, and 2,395 U·L−1 for β-glucosidase. The sequential optimisation strategy was effective and afforded increased cellulase production in the order from 3.6 to 9.5 times higher than production using nonoptimised conditions.

Optimization and production kinetics for cellulases by wild and mutant strain of Thermomyces dupontii in stirred tank reactor

“…In current project several factors including time of incubation, agitation rate, aeration, oxygen transfer, pH and inoculum size were optimized for scale up studies by thermophillic T.dupontii. Similar study has been mentioned by Carvalho et al (2014) who reported cellulaes production in a stirred tank reactor by Penicillium sp.…”

“…Besides this optimal enzyme activity also relies on the type or nature of microbial strain, their genetic makeup as well as its environmental and cultural conditions (Sudan & Bajaj, 2007). Besides this shorter fermentation time in case of mutant thermophilic T. dupontii as compared to other thermophilic indicates the stain possess the ability of economic enzyme production Carvalho et al (2014). The current findings are not in accordance with Carvalho et al (2014) who stated optimal cellulase production in the case of Penicillium at 72 hours.…”

“…Generally, bioprocesses are categorized into three main scales, i.e. laboratory scale, pilot plant and production plant (Carvalho et al, 2014). Now a day the most common bioreactor used is stirred tank reactor (STR).…”

Co-Cultivation of Penicillium sp. AKB-24 and Aspergillus nidulans AKB-25 as a Cost-Effective Method to Produce Cellulases for the Hydrolysis of Pearl Millet Stover

“…For efficient hydrolysis of lignocellulosic biomass in to reducing sugars, enzyme preparation requires balanced activities of all the components of cellulases such as endo-β-1, 4-glucanases, exo-β-1, 4-glucanase, and β-glucosidase along with xylanases [11,13]. All these enzymes act synergistically to hydrolyze lignocellulosic biomass by the creation of new accessible sites for each other and prevention of product inhibition [14]. Products obtained after hydrolysis of lignocellulosic biomass are composed mainly of glucose, xylose, cellobiose, and oligosaccharides.…”

Statistics-Based Optimization of Cellulase and Xylanase Production by the Endophytic Fungus Talaromyces Funiculosus using Agricultural Waste Materials

“…Carvalho and co-authors [33] carried out optimization of the culture media composition for strain P. funiculosum ATCC11797 in accordance with the full factorial (2 4 FFD) design. Th e se- [14,31].…”