Optimisation of a silicon/silicon dioxide substrate for a fluorescence DNA microarray

Bras, Marlène; Dugas, Vincent; Bessueille, François; Cloarec, Jean-Pierre; Martin, Jean‐René; Cabrera, Michel; Chauvet, Jean-Paul; Souteyrand, Éliane; Garrigues, M.

doi:10.1016/j.bios.2004.03.018

Cited by 75 publications

(58 citation statements)

References 17 publications

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“…The maximum achievable enhancement is in the order of 20 to 30 for multilayer stacks. 77,78 However, these values will be hard to reach for multiple fluorophores because a greater variety of excitation and emission wavelengths have to be taken into account.…”

Section: Scanning Techniquesmentioning

confidence: 99%

Optical microarray biosensing techniques

Bally

Halter

Vörös

et al. 2006

Surface & Interface Analysis

158

126

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Microarrays provide a powerful analytical tool for the simultaneous analyses of thousands of parameters, be they DNA or proteins, in a single experiment. However, a number of challenges continue to hinder the widespread application of microarray assays for analytical and diagnostic purposes. Advances in detection methods can help overcome some of these challenges by improving sensitivity and reliability in signal detection and by enabling real-time detection of binding events. Thus, the aim of this review is to highlight the most promising optical microarray biosensing techniques. The principles of techniques making use of labels, including scanner type, total internal reflection type, fiber-optics-based, and SPRenhanced fluorescence, are described, along with a brief summary of labeling strategies. State of the art label-free techniques, including imaging SPR and imaging ellipsometry, are also reviewed. Examples of microarray-based assays using each technique are given to illustrate both their usefulness and their limits of detection. Furthermore, the most competitive commercial microarray systems are presented and compared with one another in the context of their detection system. Finally, a discussion of the remaining challenges as well as trends and future applications of microarrays are presented in the context of optical sensing.

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Section: Scanning Techniquesmentioning

confidence: 99%

Optical microarray biosensing techniques

Bally

Halter

Vörös

et al. 2006

Surface & Interface Analysis

158

126

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“…Our process uses a monofunctional silane, tert-butyl-11-(dimethylamino)silylundecanoate which is an organo silazane bearing an ester function. Chemical functionalization of silica (Bras et al, 2004), PSi (Bessueille et al, 2005) and glass have been reported using this molecule from solution in pentane or from gas phase (Phaner-Goutorbe et al, 2011). As illustrated in fig.…”

Section: Porous Silicon Functionalization For Dna Sensingmentioning

confidence: 99%

New concepts of integrated photonic biosensors based on porous silicon

Li¹,

Gerelli²,

Orobtchouk³

et al. 2011

Biosensors - Emerging Materials and Applications

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“…Previous studies show that utilizing a layered substrate, e.g., SiO 2 /Si, can redirect the emission profile back to the detector for fluorescence enhancement. 21,22 SiO 2 /Si substrates are a standard in the semiconductor industry and the wide availability, well-established processing protocols, and smooth SiO 2 surface make them a promising alternative to glass slides. The glass top-layer facilitates the use of well-established surface chemistries.…”

Section: A Slide Designmentioning

confidence: 99%

Integrated imaging instrument for self-calibrated fluorescence protein microarrays

Reddington

Monroe

Ünlü

2013

Review of Scientific Instruments

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Protein microarrays, or multiplexed and high-throughput assays, monitor multiple protein binding events to facilitate the understanding of disease progression and cell physiology. Fluorescence imaging is a popular method to detect proteins captured by immobilized probes with high sensitivity and specificity. Reliability of fluorescence assays depends on achieving minimal inter-and intra-assay probe immobilization variation, an ongoing challenge for protein microarrays. Therefore, it is desirable to establish a label-free method to quantify the probe density prior to target incubation to calibrate the fluorescence readout. Previously, a silicon oxide on silicon chip design was introduced to enhance the fluorescence signal and enable interferometric imaging to self-calibrate the signal with the immobilized probe density. In this paper, an integrated interferometric reflectance imaging sensor and widefield fluorescence instrument is introduced for sensitive and calibrated microarray measurements.

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Optimisation of a silicon/silicon dioxide substrate for a fluorescence DNA microarray

Cited by 75 publications

References 17 publications

Optical microarray biosensing techniques

Optical microarray biosensing techniques

New concepts of integrated photonic biosensors based on porous silicon

Integrated imaging instrument for self-calibrated fluorescence protein microarrays

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