Optimisation of a lipid based oral delivery system containing A/Panama influenza haemagglutinin

Mann, J. Adin; Ferro, Valerie A.; Mullen, Alexander B.; Tetley, Lawrence; Mullen, Margaret; Carter, Katharine C.; Alexander, James; Stimson, W. H.

doi:10.1016/j.vaccine.2003.11.067

Cited by 61 publications

(37 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…17,18 Bile salts stabilized vesicles (bilosomes) have shown promising potential in oral vaccine delivery. 17,19,20 Bilosomes could provide both protection to the antigen from the hostile environment of GI tract as well as enable transmucosal uptake and subsequent immunization. 19 Thus, it was indicated that liposomes containing bile salts may act as more stable carriers than conventional liposomes and facilitate the transmembrane transport and absorption of drugs.…”

Section: Introductionmentioning

confidence: 99%

Enhanced oral bioavailability of cyclosporine A by liposomes containing a bile salt

Guan

Lü²,

Qi³

et al. 2011

IJN

View full text Add to dashboard Cite

The main purpose of this study was to evaluate liposomes containing a bile salt, sodium deoxycholate (SDC), as oral drug delivery systems to enhance the oral bioavailability of the poorly water-soluble and poorly permeable drug, cyclosporine A (CyA). Liposomes composed of soybean phosphatidylcholine (SPC) and SDC were prepared by a thin-film dispersion method followed by homogenization. Several properties of the liposomes including particle size, polydispersity index, and entrapment efficiency were characterized. The in vitro release of CyA from these liposomes was less than 5% at 12 hours as measured by a dynamic dialysis method. The pharmacokinetic results in rats showed improved absorption of CyA in SPC/SDC liposomes, compared with CyA-loaded conventional SPC/cholesterol (Chol) liposomes and microemulsion-based Sandimmune Neoral ® . The relative oral bioavailability of CyA-loaded SPC/SDC and SPC/Chol liposomes was 120.3% and 98.6%, respectively, with Sandimmun Neoral as the reference. The enhanced bioavailability of CyA was probably due to facilitated absorption by the liposomes containing SDC rather than improved release rate.

show abstract

Section: Introductionmentioning

confidence: 99%

Enhanced oral bioavailability of cyclosporine A by liposomes containing a bile salt

Guan

Lü²,

Qi³

et al. 2011

IJN

View full text Add to dashboard Cite

show abstract

“…Intranasal vaccines would have a detrimental effect on persons with asthma, reactive airway disease, and other chronic pulmonary or cardiovascular disorders (4). Oral vaccines therefore seem to be the safest alternative (13). Moreover, there is evidence to prove the ability of oral vaccination to prevent infection of the lungs (23) and cause transcytosis of the molecule across the cells into the circulation (24).…”

mentioning

confidence: 99%

Gastrointestinal Delivery of Baculovirus Displaying Influenza Virus Hemagglutinin Protects Mice against Heterologous H5N1 Infection

Prabakaran

Selvaraj

Prabhu

et al. 2010

J Virol

View full text Add to dashboard Cite

“…tetanus toxoid (Mann et al, 2006), the A/panama (Mann et al, 2004), Diphtheria toxoid (Shukla et al, 2011) and hepatitis B (Shukla et al, 2010). These studies all show that, by orally administering the bilosome vesicles with entrapped antigen, a mucosal immune response is elicited with specific IgA production increased.…”

Section: Introductionmentioning

confidence: 81%

“…In response to this, a range of modified lipid based vesicles, which have increased stability and increased absorption through the GIT, have been developed. Such vesicles include bilosomes, which have been shown to protect antigens from the enzymes present in the GIT and act as potent immunological adjuvants (Mann et al, 2009, Shukla et al, 2008, Mann et al, 2004. This has been attributed to the incorporation of bile salts into the vesicle bilayers, which increases resistance to degradation and disruption by digestive enzymes (Schubert et al, 1983).…”

Section: Introductionmentioning

confidence: 99%

Characterization and optimization of bilosomes for oral vaccine delivery

Wilkhu

McNeil

Anderson

et al. 2013

Journal of Drug Targeting

View full text Add to dashboard Cite

Oral vaccines offer significant benefits due to the ease of administration, better patient compliance and non-invasive, needle-free administration. However this route is marred by the harsh gastro intestinal environment which is detrimental to many vaccine formats. To address this, a range of delivery systems have been considered including bilosomes; these are bilayer vesicles constructed from non-ionic surfactants combined with the inclusion of bile salts which can stabilise the vesicles in the gastro intestinal tract by preventing membrane destabilisation. The aim of this study was to investigate the effect of formulation parameters on bilosome carriers using Design of Experiments to select an appropriate formulation to assess in vivo. Bilosomes were constructed from monopalmitoyl glycerol, cholesterol, dicetyl phosphate and sodium deoxycholate at different blends ratios. The optimised bilosome formulation was identified and the potential of this formulation as an oral vaccine delivery system were assessed in biodistribution and vaccine efficacy studies. Results showed that the larger bilosomes vesicles (~6 µm versus 2 µm in diameter) increased uptake within the Peyer's patches and were able to reduce median temperature differential change and promote a reduction in viral cell load in an influenza challenge study.3

show abstract

Optimisation of a lipid based oral delivery system containing A/Panama influenza haemagglutinin

Cited by 61 publications

References 12 publications

Enhanced oral bioavailability of cyclosporine A by liposomes containing a bile salt

Enhanced oral bioavailability of cyclosporine A by liposomes containing a bile salt

Gastrointestinal Delivery of Baculovirus Displaying Influenza Virus Hemagglutinin Protects Mice against Heterologous H5N1 Infection

Characterization and optimization of bilosomes for oral vaccine delivery

Contact Info

Product

Resources

About