Optimisation of a culture medium containing fish silage for l-lysine production by Corynebacterium glutamicum

Coello, Nereida; Montiel, Edie; Concepcion, Milagros; Christen, Pierre

doi:10.1016/s0960-8524(02)00084-6

Cited by 28 publications

(16 citation statements)

References 7 publications

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“…However, the general physiological response of C. glutamicum to complex medium (compounds) was not studied yet. To date, supplementation investigations with the strain mostly aimed at media optimization (Coello et al, 2002; Jeon et al, 2013) or usage of alternative carbon and/or nitrogen sources (Lee et al, 2014; Wendisch et al, 2016; Lange et al, 2017). For E. coli , in contrast, profound knowledge exists based on physiological investigations in Luria–Bertani (LB) broth (Sezonov et al, 2007) and due to comparable transcriptional expression analyses of late-exponential E. coli cultures grown in minimal and LB medium (Tao et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Physiological Response of Corynebacterium glutamicum to Increasingly Nutrient-Rich Growth Conditions

et al. 2018

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To ensure economic competitiveness, bioprocesses should achieve maximum productivities enabled by high growth rates (μ) and equally high substrate consumption rates (qS) as a prerequisite of sufficient carbon-to-product conversion. Both traits were investigated and improved via bioprocess engineering approaches studying the industrial work horse Corynebacterium glutamicum. Standard minimal medium CGXII with glucose as sole carbon source was supplemented with complex brain-heart-infusion (BHI) or amino acid (AA) cocktails. Maximum μ of 0.67 h-1 was exclusively observed in 37 g BHI L-1 whereas only minor growth stimulation was found after AA supplementation (μ = 0.468 h-1). Increasing glucose consumption rates (qGlc) were solely observed in certain dosages of BHI (1–10 g L-1), while 37 g BHI L-1 and AA addition revealed qGlc below the reference experiments. Moreover, BHI supplementation revealed Monod-type saturation kinetics of μ (KBHI = 2.73 g BHI L-1) referring to the preference of non-AAs as key boosting nutrients. ATP-demands under reference, 1 g BHI L-1, and AA conditions were nearly constant but halved in BHI concentrations above 5 g L-1 reflecting the energetic advantage of consuming complex nutrient components in addition to “simple” building blocks such as AAs. Furthermore, C. glutamicum revealed maximum biomass per carbon yields of about 18 gCDW C-mol-1 irrespective of the medium. In AA supplementation experiments, simultaneous uptake of 17 AAs was observed, maximum individual consumption rates determined, and L-asparagine and L-glutamine were distinguished as compounds with the highest consumption rates. Employment of the expanded stoichiometric model iMG481 successfully reproduced experimental results and revealed the importance of C. glutamicum’s transaminase network to compensate needs of limiting AA supply. Model-based sensitivity studies attributed the highest impact on μ to AAs with high ATP and NADPH demands such as L-tryptophan or L-phenylalanine.

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Section: Introductionmentioning

confidence: 99%

Physiological Response of Corynebacterium glutamicum to Increasingly Nutrient-Rich Growth Conditions

et al. 2018

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“…Fermentation of high concentrations of l -lysine is easily carried out891011121314. Currently, l -lysine has an annual production of more than 2 million tons15.…”

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confidence: 99%

Enzymatic production of 5-aminovalerate from l-lysine using l-lysine monooxygenase and 5-aminovaleramide amidohydrolase

Liu

Zhang

et al. 2014

Sci Rep

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5-Aminovalerate is a potential C5 platform chemical for synthesis of valerolactam, 5-hydroxyvalerate, glutarate, and 1,5-pentanediol. It is a metabolite of l-lysine catabolism through the aminovalerate pathway in Pseudomonas putida. l-Lysine monooxygenase (DavB) and 5-aminovaleramide amidohydrolase (DavA) play key roles in the biotransformation of l-lysine into 5-aminovalerate. Here, DavB and DavA of P. putida KT2440 were expressed, purified, and coupled for the production of 5-aminovalerate from l-lysine. Under optimal conditions, 20.8 g/L 5-aminovalerate was produced from 30 g/L l-lysine in 12 h. Because l-lysine is an industrial fermentation product, the two-enzyme coupled system presents a promising alternative for the production of 5-aminovalerate.

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“…L-Lysine is produced mainly via submerged fermentation, which represents around 80% of worldwide market. Corynebacterium glutamicum or engineered Escherichia coli are employed to carry out the fermentation steps (Coello et al 2002). The major costs involved in L-Lysine production are due to raw material followed by separation and purification steps (Whitmann and Becker 2007).…”

Section: Introductionmentioning

confidence: 99%

Development of an L-Lysine Enriched Bran for Animal Nutrition via Submerged Fermentation by Corynebacterium glutamicum using Agroindustrial Substrates

Letti

Soccol

2016

Braz. arch. biol. technol.

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L-Lysine is an essential aminoacid added as supplement for animal feed. The aim of this work was to produce an LLysine enriched bran using Brazilian agroindustrial byproducts. Both the raw material costs and purification steps were minimized. Firstly, medium composition for the growth of Corynebacterium glutamicum ATCC 21799 was optimized targeting enhanced L-Lysine production -salt, vitaminsand nitrogen sources concentrations were tested and selected. Next, UV mutant strains were generated and the best producers were used in formulated media using sugarcane molasses. It was reached a production of 9.3 g/L of L-Lysine with the optimized formulated media. This LLyisne rich broth was then impregnated and cyclically reimpregnated in pre-treated solid matrixes (sugarcane bagasse, citrus pulp, brewer spent grain, soybean husk and wheat bran). After processing, it was generated enriched brans with significant amounts of 7.0%, 8.9%, 5.9% and 8.4%, respectively), which has an interesting market potential for animal feed.

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Optimisation of a culture medium containing fish silage for l-lysine production by Corynebacterium glutamicum

Cited by 28 publications

References 7 publications

Physiological Response of Corynebacterium glutamicum to Increasingly Nutrient-Rich Growth Conditions

Physiological Response of Corynebacterium glutamicum to Increasingly Nutrient-Rich Growth Conditions

Enzymatic production of 5-aminovalerate from l-lysine using l-lysine monooxygenase and 5-aminovaleramide amidohydrolase

Development of an L-Lysine Enriched Bran for Animal Nutrition via Submerged Fermentation by Corynebacterium glutamicum using Agroindustrial Substrates

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