Optimisation of a Capillary Zone Electrophoresis Methodology for Simultaneous Analysis of Organic Aliphatic Acids in Extracts of Brachiaria brizantha

Abstract

The methodology was successfully applied to simultaneously determine citric, malic and aspartic acid in roots and leaves extract solutions of Brachiaria brizantha, demonstrating its usefulness to study aluminum tolerance.

Advance of CE and CEC in phytochemical analysis (2012–2013)

“…To optimize a CZE method for simultaneous separation of acetic, citric, formic, lactic, malic, oxalic, pyruvic, succinic, tartaric, and aspartic acid in plant extract solutions, a 3 2 full factorial design, that is, an experimental investigation with two factors and three levels, followed by a 2 3 full factorial design (three factors and two levels) were carried out. The analysis could be performed in 4 min under the optimized conditions . In addition, the optimum conditions for separation and determination of honokiol and magnolol in Magnolia officinalis and its medicinal preparation, Huoxiang Zhengqi Liquid, were obtained based on a Box‐Behnken statistical design with three factors, three levels, and 13 runs .…”

“…UV detector is the standard detection of commercial CE system, though the concentration sensitivity is low due to its shorter optical path length. Both direct (with or without derivatization) and indirect (by adding substance with strong UV absorption into BGE) UV detections were used for phytochemical analysis. For derivatization, 1‐phenyl‐3‐methyl‐5‐pyrazolone and 4‐aminobenzoic acid ethyl ester were used as derivative agents for CE analysis carbohydrates.…”

“…For derivatization, 1‐phenyl‐3‐methyl‐5‐pyrazolone and 4‐aminobenzoic acid ethyl ester were used as derivative agents for CE analysis carbohydrates. While N ‐(1‐naphthyl)ethylenediamine dihydrochloride and phthalic acid were employed as probe for analysis of peimine and peiminine in Bulbus Fritillariae Thunbergii and organic aliphatic acids in extracts of Brachiaria brizantha , respectively. A promising CE method with direct UV detection was proposed for analysis of neutral carbohydrates under extremely high alkaline conditions .…”

A Rapid Method for Total Β‐Escin Analysis in Dry, Hydroalcoholic and Hydroglycolic Extracts of Aesculus hippocastanum L. by Capillary Zone Electrophoresis

“…The most common mode of CE is capillary zone electrophoresis (CZE), which has played an important role in modern separation science since the late 1970s (Mikkers et al ., ) and currently has acquired considerable importance as a technique for separating a wide range of analytes in biological, environmental, food, pharmaceutical and medical samples (Hanrahan et al ., ). Capillary electrophoresis has several advantages over other separation techniques, such as versatility, selectivity, high efficiency, sensitivity, short analysis time, easy sample preparation (derivatisation steps are not always necessary), low cost and generally absence of organic solvents in the BGE, which suits the green chemistry principle (Xie and He ; Vaz et al ., ).…”

Chromophoreasy, an Excel-Based Program for Detection and Integration of Peaks from Chromatographic and Electromigration Techniques

“…The running conditions were based on a recent work. 24 The experiments involving CZE analysis of lactose and lactulose standards based on literature reports 25 were performed in an Agilent 7100 CE system controlled by Agilent ChemStation software (rev. B.04.03) and equipped with a DAD and a lab-made capacitively coupled contactless conductivity detector (C 4 D).…”