Optimisation and sensitivity of single-stranded conformation polymorphism for the detection of hepatitis C virus quasi-species

McKechnie, Victoria M.; Mccruden, E. A. B.

doi:10.1016/s0166-0934(00)00279-2

Cited by 5 publications

(2 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It would also be useful to further characterize the viral subpopulations excreted in the digestive and respiratory tracts, paying particular attention to the different contact groups. For such large studies, techniques using differential gel electrophoresis mobility, such as single-strand conformation polymorphism (Mc Kechnie & Mc Cruden, 2001), would be useful to obtain an overview on viral heterogeneity. Several studies using only pPMV-1 are needed to fully understand its pathogenesis.…”

Section: C (1) -----------------------------------------------------mentioning

confidence: 99%

Molecular study of the quasispecies evolution of a typical pigeon paramyxovirus type 1 after serial passages in pigeons by contact

Barbezange

Jestin

2005

Avian Pathology

View full text Add to dashboard Cite

The quasispecies nature of a typical pigeon paramyxovirus type 1 (pPMV-1) was, for the first time, studied under conditions close to the natural infectious environment. The virus was serially passaged in pigeons by successive contacts. Viral heterogeneity was analysed in the kidneys and brain of five pigeons from the last contact, by reverse transcriptase-polymerase chain reactions performed on RNA directly extracted from the organ and targeting the P and HN genes of the virus. The viral diversity following in vivo passage was found to be different from that in the inoculum, but demonstrated the reality of the quasispecies concept for pPMV-1 strains. Moreover, some aberrant genomic RNAs comprising insertions in the P gene editing site or deletions in the HN gene were also detected, with possible consequences for the pathogenicity and infectivity of the virus.

show abstract

Section: C (1) -----------------------------------------------------mentioning

confidence: 99%

Molecular study of the quasispecies evolution of a typical pigeon paramyxovirus type 1 after serial passages in pigeons by contact

Barbezange

Jestin

2005

Avian Pathology

View full text Add to dashboard Cite

show abstract

“…Various methods for mutation detection have been developed and used in clinical diagnosis of genetic disorders, cancer, viral genome analysis, and for pharmacogenetic studies (5)(6)(7)(8)(9)(10)(11). Among them are denaturing gradient gel electrophoresis (DGGE), singlestrand conformation polymorphism (SSCP), chemical/enzymatic mismatch cleavage, restriction fragment length polymorphism (RFLP), allele-specific oligonucleotide hybridization, allele-specific amplification, capillary electrophoresis and DNA sequencing.…”

Section: Introductionmentioning

confidence: 99%

Increasing the Sensitivity of Single-Strand Conformation Polymorphism Analysis of the LDLR Gene Mutations in Brazilian Patients with Familial Hypercholesterolemia

Salazar¹,

Hirata²,

Hirata³

2002

Clinical Chemistry and Laboratory Medicine

View full text Add to dashboard Cite

Mutations in the low-density lipoprotein receptor (LDLR) gene cause familial hypercholesterolemia (FH), one of the most common single gene disorders. It is thought that FH affects approximately 1 of 500 individuals in most populations. Single-strand conformation polymorphism (SSCP) analysis is widely used to detect mutations in the LDLR gene. However, several factors such as temperature, pH, running time, gel composition and size of the DNA fragments can influence its sensitivity. We have optimized the electrophoretic conditions to screen mutations in the promoter region and exons 1-18 of the LDLR gene by varying temperature (5 degrees C, 8 degrees C, 12 degrees C and 15 degrees C), voltage (300 to 600 V), and running time (1 to 4 hours) in the semi-automated GenePhor system (Amersham Biosciences). The efficiency of the method was evaluated by using 30 positive controls (DNA samples with mutations and polymorphisms in the LDLR gene, previously characterized) and DNA samples from 90 Brazilian patients with FH. Our results show that the use of two temperatures (5 degrees C and 15 degrees C) in combination with other optimized conditions resulted in high mutation detection rate (97%), which was considered appropriate for routine screening. Therefore, this strategy could be useful for the diagnosis of genetic disorders, cancer, and for pharmacogenetic studies.

show abstract

Quasispecies as Predictive Response Factors for Antiviral Treatment in Patients with Chronic Hepatitis C

et al. 2006

View full text Add to dashboard Cite

The object of this study was to evaluate the viral factor, especially the quasispecies, as predictive of sustained virologic response. We studied the quasispecies, genotype, viral load, and hepatitis C (HCV) cAg in 41 patients with chronic hepatitis C treated with interferon and in 84 with interferon and ribavirin. In the interferon group, responders presented a lower viral load. From logistic regression analysis of patients treated with interferon plus ribavirin, independent predictors for sustained virologic response were genotype 3a, a low baseline viral load and

show abstract

Optimisation and sensitivity of single-stranded conformation polymorphism for the detection of hepatitis C virus quasi-species

Cited by 5 publications

References 22 publications

Molecular study of the quasispecies evolution of a typical pigeon paramyxovirus type 1 after serial passages in pigeons by contact

Molecular study of the quasispecies evolution of a typical pigeon paramyxovirus type 1 after serial passages in pigeons by contact

Increasing the Sensitivity of Single-Strand Conformation Polymorphism Analysis of the LDLR Gene Mutations in Brazilian Patients with Familial Hypercholesterolemia

Quasispecies as Predictive Response Factors for Antiviral Treatment in Patients with Chronic Hepatitis C

Contact Info

Product

Resources

About