Molecular study of the quasispecies evolution of a typical pigeon paramyxovirus type 1 after serial passages in pigeons by contact

Abstract

The quasispecies nature of a typical pigeon paramyxovirus type 1 (pPMV-1) was, for the first time, studied under conditions close to the natural infectious environment. The virus was serially passaged in pigeons by successive contacts. Viral heterogeneity was analysed in the kidneys and brain of five pigeons from the last contact, by reverse transcriptase-polymerase chain reactions performed on RNA directly extracted from the organ and targeting the P and HN genes of the virus. The viral diversity following in vivo passage was found to be different from that in the inoculum, but demonstrated the reality of the quasispecies concept for pPMV-1 strains. Moreover, some aberrant genomic RNAs comprising insertions in the P gene editing site or deletions in the HN gene were also detected, with possible consequences for the pathogenicity and infectivity of the virus.

Isolation and Detection of Newcastle Disease Virus From Field Outbreaks in Broiler and Layer Chickens by Reverse Transcription–polymerase Chain Reaction

“…Newcastle disease virus (NDV) which is also called avian paramyxovirus serotype 1 (APMV-1) is a single stranded, enveloped, negative sense RNA virus belonging to the genus Rubulavirus of subfamily Paramyxovirinae and family Paramyxoviridiae (Barbezange and Jestin, 2005;Mayo, 2002). Based on pathogenic and virulence properties, NDV is categorized into three major pathotypic strains i.e.…”

Genome-wide association study of antibody level response to NDV and IBV in Jinghai yellow chicken based on SLAF-seq technology

“…ND results in dyspnoea, dysentery, neurological disorders, and serosal and mucosal haemorrhages, with high morbidity and mortality in chickens (Aldous and Alexander 2001;Barbezange and Jestin 2005). In addition, it can lead to substantial economic losses due to international trade restrictions and closures.…”

Detection of Newcastle Disease Virus by Immunohistochemistry on the Brains of Laying Birds with Clinical Signs Torticollis and Curled Toe Paralysis

“…NDV belonging to the genus Rubulavirus and family Paramyxoviridiae [4]. Based on the pathogenic and virulence properties, NDV is categorized into three major pathotypic strains e.g.…”