Optimisation and Evaluation of Zinc in Food Samples by Cloud Point Extraction and Spectrophotometric Detection

Azizi, Nurul Azwa Mohd; Rahim, Nurul Yani; Raoov, Muggundha; Asman, Saliza

doi:10.24191/srj.v16i1.5551

Cited by 4 publications

(2 citation statements)

References 21 publications

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“…TX-114 is a cost-effective surfactant, and forms micelles at room temperature. CPE methods using TX-114, ethylenediaminetetraacetic acid, or 8-hydroxyquinoline were used to analyze trace NPs under aqueous conditions or minerals in tissues, resulting in their separation in the precipitated, surfactant-rich phase [27,28]. In this case, pretreatments to digest organic matrices were essentially required prior to CPE application.…”

Section: Introductionmentioning

confidence: 99%

Determination of Two Differently Manufactured Silicon Dioxide Nanoparticles by Cloud Point Extraction Approach in Intestinal Cells, Intestinal Barriers and Tissues

Yoo

Jeon

Choi

2021

IJMS

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Food additive amorphous silicon dioxide (SiO2) particles are manufactured by two different methods—precipitated and fumed procedures—which can induce different physicochemical properties and biological fates. In this study, precipitated and fumed SiO2 particles were characterized in terms of constituent particle size, hydrodynamic diameter, zeta potential, surface area, and solubility. Their fates in intestinal cells, intestinal barriers, and tissues after oral administration in rats were determined by optimizing Triton X-114-based cloud point extraction (CPE). The results demonstrate that the constituent particle sizes of precipitated and fumed SiO2 particles were similar, but their aggregate states differed from biofluid types, which also affect dissolution properties. Significantly higher cellular uptake, intestinal transport amount, and tissue accumulation of precipitated SiO2 than of fumed SiO2 was found. The intracellular fates of both types of particles in intestinal cells were primarily particle forms, but slowly decomposed into ions during intestinal transport and after distribution in the liver, and completely dissolved in the bloodstream and kidneys. These findings will provide crucial information for understanding and predicting the potential toxicity of food additive SiO2 after oral intake.

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Section: Introductionmentioning

confidence: 99%

Determination of Two Differently Manufactured Silicon Dioxide Nanoparticles by Cloud Point Extraction Approach in Intestinal Cells, Intestinal Barriers and Tissues

Yoo

Jeon

Choi

2021

IJMS

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“…However, these studies used ethylenediaminetetraacetic acid (EDTA) or 8-hydroxyquinoline as chelating agents for precipitation of Al or Zn ions in precipitated surfactant-rich phase by CPE [34,35]. Moreover, the CPE method was applied after pre-treatments such as filtering, nitric acid treatment, and dry-ashing were applied in order to digest organic matrices in foods [34,35]. No attempt has been made to detect ZnO in its particle form in complex systems, such as in foods or biomatrices.…”

Section: Introductionmentioning

confidence: 99%

Fate Determination of ZnO in Commercial Foods and Human Intestinal Cells

Jeon

Jin

Choi

2020

IJMS

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(1) Background: Zinc oxide (ZnO) particles are widely used as zinc (Zn) fortifiers, because Zn is essential for various cellular functions. Nanotechnology developments may lead to production of nano-sized ZnO, although nanoparticles (NPs) are not intended to be used as food additives. Current regulations do not specify the size distribution of NPs. Moreover, ZnO is easily dissolved into Zn ions under acidic conditions. However, the fate of ZnO in commercial foods or during intestinal transit is still poorly understood. (2) Methods: We established surfactant-based cloud point extraction (CPE) for ZnO NP detection as intact particle forms using pristine ZnO-NP-spiked powdered or liquid foods. The fate determination and dissolution characterization of ZnO were carried out in commercial foods and human intestinal cells using in vitro intestinal transport and ex vivo small intestine absorption models. (3) Results: The results demonstrated that the CPE can effectively separate ZnO particles and Zn ions in food matrices and cells. The major fate of ZnO in powdered foods was in particle form, in contrast to its ionic fate in liquid beverages. The fate of ZnO was closely related to the extent of its dissolution in food or biomatrices. ZnO NPs were internalized into cells in both particle and ion form, but dissolved into ions with time, probably forming a Zn–ligand complex. ZnO was transported through intestinal barriers and absorbed in the small intestine primarily as Zn ions, but a small amount of ZnO was absorbed as particles. (4) Conclusion: The fate of ZnO is highly dependent on food matrix type, showing particle and ionic fates in powdered foods and liquid beverages, respectively. The major intracellular and intestinal absorption fates of ZnO NPs were Zn ions, but a small portion of ZnO particle fate was also observed after intestinal transit. These findings suggest that the toxicity of ZnO is mainly related to the Zn ion, but potential toxicity resulting from ZnO particles cannot be completely excluded.

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Utilization of a novel PVC- optical sensor for high sensitive and selective determination of zinc ion in real samples

Alshehri,

El-Feky,

Askar

et al. 2024

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

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Optimisation and Evaluation of Zinc in Food Samples by Cloud Point Extraction and Spectrophotometric Detection

Cited by 4 publications

References 21 publications

Determination of Two Differently Manufactured Silicon Dioxide Nanoparticles by Cloud Point Extraction Approach in Intestinal Cells, Intestinal Barriers and Tissues

Determination of Two Differently Manufactured Silicon Dioxide Nanoparticles by Cloud Point Extraction Approach in Intestinal Cells, Intestinal Barriers and Tissues

Fate Determination of ZnO in Commercial Foods and Human Intestinal Cells

Utilization of a novel PVC- optical sensor for high sensitive and selective determination of zinc ion in real samples

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