Optimal Surface Aeration Rate for Bioethanol Production from the Hydrolysate of Seaweed Sargassum sagamianum Using Pichia stipitis

이상은,; 김혜지,; 최운용,; 강도형,; 이현용,; 정경환,

doi:10.7841/ksbbj.2011.26.4.311

Cited by 3 publications

(6 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this section, we investigated the performance of bioethanol production in the repeated-batch fermentor at surface aeration rates ranging from 30 to 100 ml/min in a working volume of 880 ml in a 2.5 l fermentor. A surface aeration rate of 100 ml/min corresponds to an oxygen transfer coefficient of about 0.053 (min -1 ) [17]. This value was remarkably lower than the value obtained when the shaking flask culture was conducted.…”

Section: Practical and Cost-effective Medium For Bioethanol Productiomentioning

confidence: 66%

“…In our previous study, we investigated the optimal surface aeration rate in the repeated-batch fermentor culture for bioethanol production using the hydrolysate of seaweed S. sagamianum [17]. The optimal surface aeration rates were determined to be between 30 and 100 ml/min in a working volume of 880 ml in a 2.5 l fermentor.…”

Section: Practical and Cost-effective Medium For Bioethanol Productiomentioning

confidence: 99%

“…The seed culture was performed in a 250 ml Erlenmeyer flask with 50 ml of YPD medium (yeast extract, 10 g/l; peptone, 20 g/l; glucose, 20 g/l) in a shaking incubator at 30 o C and 150 rpm. The fermentor culture was performed in a 2.5 l jar fermentor (KoBiotech, Republic of Korea) with an initial working volume of 880 ml, which was prepared by mixing 800 ml of CSH medium [CSL, 20 ml/l; (NH 4 ) 2 SO 4 , 1.2 g/l; KH 2 PO 4 , 2.4 g/l; and MgSO 4 •7H 2 O, 1.2 g/l were dissolved in the hydrolysate of seaweed S. sagamianum] [17,25] and 80 ml of seed culture. During fermentation, the temperature, pH, and agitation speed were maintained at 30 o C, 5.0, and 200 rpm, respectively.…”

mentioning

confidence: 99%

“…All measurements were conducted three times (n=3) using the same sample, and the average and standard deviations were calculated. The data are partially quoted from our previous work [17].…”

mentioning

confidence: 99%

See 3 more Smart Citations

Development of a Practical and Cost-Effective Medium for Bioethanol Production from the Seaweed Hydrolysate in Surface-Aerated Fermentor by Repeated-Batch Operation

Lee

Lee²,

Shin³

et al. 2012

J. Microbiol. Biotechnol.

Self Cite

View full text Add to dashboard Cite

To develop a practical and cost-effective medium for bioethanol production from the hydrolysate of seaweed Sargassum sagamianum, we investigated the feasibility and performance of bioethanol production in CSL (cornsteep liquor)-containing medium, where yeast Pichia stipitis was used and the repeated batch was carried out in a surface-aerated fermentor. The optimal medium replacement time during the repeated operation was determined to be 36 h, and the surface aeration rates were 30 and 100 ml/min. Under these conditions, the repeatedbatch operation was successfully carried out for 6 runs (216 h), in which the maximum bioethanol concentrations reached about 11-12 g/l at each batch operation. These results demonstrated that bioethanol production could be carried out repeatedly and steadily for 216 h. In these experiments, the total cumulative bioethanol production was 57.9 g and 58.0 g when the surface aeration rates were 30 ml/min and 100 ml/min, respectively. In addition, the bioethanol yields were 0.43 (about 84% of theoretical value) and 0.44 (about 86% of theoretical value) when the surface aeration rates were 30 ml/min and 100 ml/min, respectively. CSL was successfully used as a medium ingredient for the bioethanol production from the hydrolysate of seaweed Sargassum sagamianum, indicating that this medium may be practical and cost-effective for bioethanol production.

show abstract

Section: Practical and Cost-effective Medium For Bioethanol Productiomentioning

confidence: 66%

Section: Practical and Cost-effective Medium For Bioethanol Productiomentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Development of a Practical and Cost-Effective Medium for Bioethanol Production from the Seaweed Hydrolysate in Surface-Aerated Fermentor by Repeated-Batch Operation

Lee

Lee²,

Shin³

et al. 2012

J. Microbiol. Biotechnol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…The temperature, pH, and agitation speed were maintained at 30 o C, 5.0, and 200 rpm, respectively. The surface aeration protocol used has been described previously [24,26,37].…”

Section: Surface-aerated Fermentor Culturementioning

confidence: 99%

Two-Step Process Using Immobilized Saccharomyces cerevisiae and Pichia stipitis for Ethanol Production from Ulva pertusa Kjellman Hydrolysate

Lee

Kim²,

Choi³

et al. 2013

J. Microbiol. Biotechnol.

Self Cite

View full text Add to dashboard Cite

We established a two-step production process using immobilized S. cerevisiae and P. stipitis yeast to produce ethanol from seaweed (U. pertusa Kjellman) hydrolysate. The process was designed to completely consume both glucose and xylose. In particular, the yeasts were immobilized using DEAE-corncob and DEAE-cotton, respectively. The first step of the process included a continuous column reactor using immobilized S. cerevisiae, and the second step included a repeated-batch reactor using immobilized P. stipitis. It was verified that the glucose and xylose in 20 L of medium containing the U. pertusa Kjellman hydrolysate was converted completely to about 5.0 g/l ethanol through the two-step process, in which the overall ethanol yield from total reducing sugar was 0.37 and the volumetric ethanol productivity was 0.126 g/ l/h. The volumetric ethanol productivity of the two-step process was about 2.7 times greater than that when P. stipitis was used alone for ethanol production from U. pertusa Kjellman hydrolysate. In addition, the overall ethanol yield from glucose and xylose was superior to that when P. stipitis was used alone for ethanol production. This two-step process will not only contribute to the development of an integrated process for ethanol production from glucose and xylose-containing biomass hydrolysates, but could also be used as an alternative method for ethanol production.

show abstract

Immobilization of Yeast Pichia stipitis for Ethanol Production

Lee¹,

Lee²,

Kim³

et al. 2012

Journal of Life Science

Self Cite

View full text Add to dashboard Cite

In this study, DEAE-cotton [derivatized by 2-(diethylamino)ethyl chloride hydrochloride (DEAE·HCl)] was prepared as a carrier for immobilized Pichia stipitis for ethanol production. When cotton was derivatized with 0.5 M DEAE·HCl, the yeast cell suspension was adsorbed at 100% of the initial cell OD600. The adsorbed yeast cells were estimated to be 101.8 mg-dry cells/g-DEAE-cotton. In particular, when a flask culture using the immobilized yeast cells was conducted in a glucose and xylose-containing medium, the yeast cells on the DEAE-cotton gradually produced ethanol, according to glucose and xylose consumption; the ethanol yield was approximately 0.33 g-ethanol/g-monosaccharide. Because DEAE-cotton was successfully used as a carrier for ethanol production from a glucose and xylose-containing medium, we expect that this bioethanol production process may be used for the bioethanol production process from the hydrolysate of lignocellulosic biomass. All the results of DEAE-cotton were compared with those of DEAE-cellulose as a carrier for immobilization.

show abstract

Optimal Surface Aeration Rate for Bioethanol Production from the Hydrolysate of Seaweed Sargassum sagamianum Using Pichia stipitis

Cited by 3 publications

References 15 publications

Development of a Practical and Cost-Effective Medium for Bioethanol Production from the Seaweed Hydrolysate in Surface-Aerated Fermentor by Repeated-Batch Operation

Development of a Practical and Cost-Effective Medium for Bioethanol Production from the Seaweed Hydrolysate in Surface-Aerated Fermentor by Repeated-Batch Operation

Two-Step Process Using Immobilized Saccharomyces cerevisiae and Pichia stipitis for Ethanol Production from Ulva pertusa Kjellman Hydrolysate

Immobilization of Yeast Pichia stipitis for Ethanol Production

Contact Info

Product

Resources

About