Optimal exponential feeding strategy for dual-substrate biostimulation of phenol degradation using Cupriavidus taiwanensis

Treatment of phenol-contaminated soil by Corynebacterium glutamicum and toxicity removal evaluation

“…This population size corresponded to the growth status of a batch culture of C. glutamicum at mid-exponential phase [12]. Based on several reports, phenol degradation actively occurs during the mid-exponential phase in a batch culture experiment of bacteria [1,7,12]. In contrast, 7.6 log 10 CFU mL −1 of C. glutamicum did not improve the efficiency of phenol degradation in contaminated soils.…”

“…Chen et al [7] reported that phenol degradation capacity is strongly dependent upon cell population size per unit volume. In the present study, the initial population of C. glutamicum was determined for the effective degradation of phenol in phenolcontaminated soils.…”

“…As shown in Table 1, the efficiency of phenol degradation by C. glutamicum was nearly 95% when the soil was spiked with phenol (8.48 mM); however, high concentrations of phenol (21.20 mM) decreased the efficiency to below 17%. In general, phenol causes membrane and protein damage to bacteria [12], indicating that the bioavailability of phenol in C. glutamicum is inhibited by high concentrations of phenol due to severe toxicity [7,12]. Fig.…”

“…Currently the primary method for its removal is the use of various biological approaches, so-called bioremediation technologies [4][5][6]. Of the techniques available for the removal of phenols from soil, biodegradation by soil bacteria is environmentally friendly and cost-effective [7,8]. Recently Corynebacterium glutamicum, an industrial soil microorganism important for amino acid production, was reported to assimilate various aromatic compounds [9,10].…”

Optimization of Soluble Organic Selenium Accumulation during Fermentation of Flammulina velutipes Mycelia

“…So, development of a suitable feeding strategy is critical in fed-batch cultivation. Exponential feeding is a simple method that allows cells to grow at a constant growth rate (Kim et al, 2004), and exponential feeding of substrates has been widely applied during fermentation (Ding and Tan, 2006;Miguel et al, 2007;Chen et al, 2009). However, no constant specifi c growth rate of fi lamentous fungi can be assumed, when growth takes place in the form of pellets (Lejeune and Baron, 1998).…”