Optimal design of affinity membrane chromatographic columns

Tejeda, Armando; Ortega, Jaime; Magaña, Ignacio; Guzmán, Roberto

doi:10.1016/s0021-9673(98)00898-x

Cited by 29 publications

(10 citation statements)

References 18 publications

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“…Therefore, to assure nanofibre use is accessible and applicable for many markets, an ability to control the production must be established. This study is particularly relevant to membrane operations which are commonly limited by poor membrane pore size uniformity and axial and radial diffusion which results in poor system dispersion, yielding low utilisation of membrane capacity [16,17]. Previous studies have highlighted the importance of designing a membrane with regards to the proposed system and operating conditions, optimising the membrane pore size by balancing mass transfer against and fouling issues [18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Nanofibre fabrication in a temperature and humidity controlled environment for improved fibre consistency

2011

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Fabricating nanofibres with reproducible characteristics is an important demand in the membrane industry in order to establish commercial viability. In this study, the effect of controlled atmospheric conditions on electrospun cellulose acetate (CA) nanofibres was evaluated for temperatures ranging 17.5-32.5°C and relative humidity ranging 20-70%. CA solution (0.2 g/mL) in a solvent mixture of acetone/dimethylformamide/ethanol (2:2:1) was electrospun into nonwoven fibre mesh with the fibre diameter ranging from 150 nm to 1 lm. The resulting nanofibres were analysed by differential scanning calorimetry, showing a correlation of reducing melt enthalpy with increasing atmospheric temperature. The opposite was seen with increasing atmospheric humidity, which conferred increasing melt enthalpy. Analysis of scanning electron microscopy images provided a correlation of reducing average fibre diameter with increasing atmospheric temperature and increasing fibre diameter with increasing atmospheric humidity. These results correlate with the melt enthalpy results, suggesting that finer CA nanofibres infer a lower melt enthalpy. Together these studies provide strong evidence that the controlled atmospheric conditions affect the fibre diameter of the resulting electrospun nanofibres. A salient observation in this study was that increased humidity reduced the effect of fibre beading yielding a more consistent and therefore better quality of fibre. This has apparent implications for the reproducibility of nanofibre production and offers a new method of controlling fibre morphology. This study has highlighted the requirement to control atmospheric conditions during the electrospinning process to fabricate reproducible fibre mats.

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Section: Introductionmentioning

confidence: 99%

Nanofibre fabrication in a temperature and humidity controlled environment for improved fibre consistency

2011

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“…Based on the analysis of the adsorption kinetics [6][7][8][9], basic membranes should have homogeneously microporous/macroporous structure with a large internal surface area, high interconnectivity and mechanical stability, which is usually owed to the feature of microfiltration membrane. Adsorptive membrane substrates should be mechanically resilient and resistant to solvents used to activate coupling and not participate in secondary hydrophobic adsorption, which produces non-specific retention that interferes with product resolution or can lead to the denaturation of biopolymers.…”

Section: Introductionmentioning

confidence: 99%

Preparation and performance of cellulose acetate/polyethyleneimine blend microfiltration membranes and their applications

Chen

Deng

Chen

et al. 2004

Journal of Membrane Science

173

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“…The integration of membrane and affinity chromatography provides a number of advantages over traditional affinity chromatography with porousw x bead-packed columns, especially with regard to time and activity recovery 49,50 . w x Tejeda et al 51 have developed a method for optimal affinity column design, based on the solution of the Thomas kinetic model for frontal analysis in membrane column adsorption. The method has allowed choosing suitable membrane operating conditions, column dimensions, and processing time to maximize the throughput when an operating capacity restriction in the range of 80-95% of the column capacity is used.…”

Section: Introductionmentioning

confidence: 99%