Optics of Erythrocytes

Light scattering by neutrophils: model, simulation, and experiment

“…The location of the last peak in this spectrum has a good linear correlation with the cell diameter, which was shown by extensive numerical simulations for spheres 23 and concentric spheres. 8 Using the proportionality coefficient determined from this numerical simulation, spectral sizing was empirically applied to erythrocytes, 24 and leukocytes, 14 producing meaningful results. However, it has never been verified for granulated cells.…”

Quantitative analysis of morphological alterations in Plasmodium falciparum infected red blood cells through theoretical interpretation of spectral measurements

“…Yet, the application of the traditional spectroscopy methods such as angular light scattering (Eremina et al, 2005;Tarasov et al, 2007) and integrating sphere diffuse transmittance (Meinke et al, 2005;Naqvi et al, 2004) to the quantitative analysis of the malaria infected RBCs remains difficult. For instance, the interpretation approaches to the angular light scattering measurements conducted at single or few wavelength(s) neglect the effect of absorption and are hurdled by the biconcave shape and the orientation of the RBCs relative to the source that heavily influence the light scattering pattern at observation angles greater than 51 (Eremina et al, 2005;Tarasov et al, 2007). The exact numerical modelling of angular light scattering from true RBC shape is computationally cumbersome (Karlsson et al, 2005;Lu et al, 2007;Nilsson et al, 1998) whereas the approximations to the RBC scattering behaviour using spheres, ellipsoids, and oblate disks have provided only adequate results (Tarasov et al, 2007).…”

“…For instance, the interpretation approaches to the angular light scattering measurements conducted at single or few wavelength(s) neglect the effect of absorption and are hurdled by the biconcave shape and the orientation of the RBCs relative to the source that heavily influence the light scattering pattern at observation angles greater than 51 (Eremina et al, 2005;Tarasov et al, 2007). The exact numerical modelling of angular light scattering from true RBC shape is computationally cumbersome (Karlsson et al, 2005;Lu et al, 2007;Nilsson et al, 1998) whereas the approximations to the RBC scattering behaviour using spheres, ellipsoids, and oblate disks have provided only adequate results (Tarasov et al, 2007). Multiwavelength diffuse transmittance employs the integrating sphere or similar optical configurations with spectrometers that collect light attenuated over a broad range of scattering angles simultaneously thereby averaging out the scattering component of the transmitted light.…”

“…We utilized simultaneous multi-wavelength measurements of both absorption and forward scattered light (i.e., the observation angle is less than 21), for which the ellipsoid approximation of the RBC shape is quantitatively effective when applied to the measurements at observation angles less than 41 (Tarasov et al, 2007). We applied a multilayered complex cell structure to account for the differences in the optical properties of the IRBC components.…”

Building up spectral libraries for mapping erythrocytes by hyperspectral dark field microscopy

“…Therefore, our work was first focused on assessing the HDFM protocol for red blood cell (RBC) imaging and developing the spectral library for healthy human RBCs. The morphology and optical characteristics of erythrocytes have been widely studied by numerous research groups [8,23,27]. Spectral characterization and information obtained from RBC can be further applied to the evaluation of health and disease conditions, since pathological status can alter the structure and molecular content of RBC [4].…”