Light scattering by neutrophils: model, simulation, and experiment

Orlova, Darya; Yurkin, Maxim A.; Hoekstra, Alfons G.; Maltsev, Valeri P.

doi:10.1117/1.2992140

Cited by 32 publications

(28 citation statements)

References 22 publications

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“…This feature is realized by measurement of a mixture of unknown particles and polymer microspheres. 39 The LSP of the polymer microsphere, measured with the SFC, gives a high accuracy agreement with the Mie theory. 40 This allows calibration in absolute lightscattering units.…”

Section: Differential Light-scattering Cross Section Of Lymphocytessupporting

confidence: 50%

Is there a difference between T- and B-lymphocyte morphology?

et al. 2009

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General rightsIt is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), other than for strictly personal, individual use, unless the work is under an open content license (like Creative Commons). Disclaimer/Complaints regulationsIf you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library: http://uba.uva.nl/en/contact, or a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible. Abstract. We characterize T-and B-lymphocytes from several donors, determining cell diameter, ratio of nucleus to cell diameter, and refractive index of the nucleus and cytoplasm for each individual cell. We measure light-scattering profiles with a scanning flow cytometer and invert the signals using a coated sphere as an optical model of the cell and by relying on a global optimization technique. The main difference in morphology of T-and B-lymphocytes is found to be the larger mean diameters of the latter. However, the difference is smaller than the natural biological variability of a single cell. We propose nuclear inhomogeneity as a possible reason for the deviation of measured light-scattering profiles from real lymphocytes from those obtained from the coated sphere model. © 2009 Society of Photo-Optical Instrumentation Engineers.

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Section: Differential Light-scattering Cross Section Of Lymphocytessupporting

confidence: 50%

Is there a difference between T- and B-lymphocyte morphology?

et al. 2009

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“…The outcome could be very important new insights that can expand our knowledge about neutrophil function and malfunction, broaden our understanding of the human immune system in health and disease with implications for etiologic, diagnostic and therapeutic considerations in multiple human diseases. (20,21,26) circulating pool in bloodstream signalling between compartments, priming and depriming (22,23,26) age time in circulation maturation, differentiation (28,54) cell size in diameter activation stage (36,40,62) cell shape formation of pseudopods different capillary transit time, adhesion, (trans-)migration (4,35,69,99) nuclear size in diameter different gene expression, NET formation (25,55) nuclear shape banded / (multi-)lobulated maturation, differentiation (10,11) activation stage in/decreased deformability different capillary transit time, (trans-)migration, phagocytosis (6,10,30,46,77) surface markers e.g. CD18, CD66a, CD66b differentiation, signalling, adhesion, phagocytosis (15,21,23,26) …”

Section: Discussionmentioning

confidence: 99%

Mechanotransduction in neutrophil activation and deactivation

Ekpenyong

Toepfner

Chilvers

et al. 2015

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research

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Mechanotransduction refers to the processes through which cells sense mechanical stimuli by converting them to biochemical signals and, thus, eliciting specific cellular responses. Cells sense mechanical stimuli from their 3D environment, including the extracellular matrix, neighboring cells and other mechanical forces. Incidentally, the emerging concept of mechanical homeostasis,long term or chronic regulation of mechanical properties, seems to apply to neutrophils in a peculiar manner, owing to neutrophils' ability to dynamically switch between the activated/primed and deactivated/deprimed states. While neutrophil activation has been known for over a century, its deactivation is a relatively recent discovery. Even more intriguing is the reversibility of neutrophil activation and deactivation. We review and critically evaluate recent findings that suggest physiological roles for neutrophil activation and deactivation and discuss possible mechanisms by which mechanical stimuli can drive the oscillation of neutrophils between the activated and resting states. We highlight several molecules that have been identified in neutrophil mechanotransduction, including cell adhesion and transmembrane receptors, cytoskeletal and ion channel molecules. The physiological and pathophysiological implications of such mechanically induced signal transduction in neutrophils are highlighted as a basis for future work. This article is part of a Special Issue entitled: Mechanobiology.

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“…Orlova et al (2008) even validate their computed scattering results by comparisons to measurements performed with a scanning flow cytometer. Indeed, several approaches in addition to flow cytometers (Jacobs et al, 2009;Strokotov et al, 2011) have been taken towards measuring scattering by individual particles: for example, air flow systems measuring two-dimensional scattering patterns (Kaye et al, 2007) and digital holographic imaging (Berg and Videen, 2011).…”

Section: H Lindqvist Et Al: Scattering By Realistic Inhomogeneous mentioning

confidence: 96%

“…Finally, we note that the stereogrammetric approach to model scattering by single dust particles more realistically has some analogies to the confocal microscopy measurements method used by Brock et al (2006) and Orlova et al (2008) to model light scattering by biological particles: both methods result in a retrieved, three-dimensional model shape for a single particle. Orlova et al (2008) even validate their computed scattering results by comparisons to measurements performed with a scanning flow cytometer.…”

Section: H Lindqvist Et Al: Scattering By Realistic Inhomogeneous mentioning

confidence: 99%

Single scattering by realistic, inhomogeneous mineral dust particles with stereogrammetric shapes

et al. 2014

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Abstract. Light scattering by single, inhomogeneous mineral dust particles was simulated based on shapes and compositions derived directly from measurements of real dust particles instead of using a mathematical shape model. We demonstrate the use of the stereogrammetric shape retrieval method in the context of single-scattering modelling of mineral dust for four different dust types -all of them inhomogeneous -ranging from compact, equidimensional shapes to very elongated and aggregate shapes. The three-dimensional particle shapes were derived from stereo pairs of scanningelectron microscope images, and inhomogeneous composition was determined by mineralogical interpretation of localized elemental information based on energy-dispersive spectroscopy. Scattering computations were performed for particles of equal-volume diameters, from 0.08 µm up to 2.8 µm at 550 nm wavelength, using the discrete-dipole approximation. Particle-to-particle variation in scattering by mineral dust was found to be quite considerable and was not well reproduced by simplified shapes of homogeneous spheres, spheroids, or Gaussian random spheres. Effective-medium approximation results revealed that particle inhomogeneity should be accounted for even for small amounts of absorbing media (here up to 2 % of the volume), especially when considering scattering by inhomogeneous particles at size parameters 3 < x < 8. When integrated over a log-normal size distribution, the linear depolarization ratio and singlescattering albedo were also found to be sensitive to inhomogeneity. The methodology applied is work-intensive and the light-scattering method used quite limited in terms of size parameter coverage. It would therefore be desirable to find a sufficiently accurate but simpler approach with fewer limitations for single-scattering modelling of dust. For validation of such a method, the approach presented here could be used for producing reference data when applied to a suitable set of target particles.

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Light scattering by neutrophils: model, simulation, and experiment

Cited by 32 publications

References 22 publications

Is there a difference between T- and B-lymphocyte morphology?

Is there a difference between T- and B-lymphocyte morphology?

Mechanotransduction in neutrophil activation and deactivation

Single scattering by realistic, inhomogeneous mineral dust particles with stereogrammetric shapes

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