Optically-induced-dielectrophoresis (ODEP)-based cell manipulation in a microfluidic system for high-purity isolation of integral circulating tumor cell (CTC) clusters based on their size characteristics

Chiu, Tzu-Keng; Chao, A-Ching; Chou, Wen-Pin; Liao, Chia‐Jung; Wang, Hung‐Ming; Chang, Jyun-Huan; Chen, Ping‐Hei; Wu, Min‐Hsien

doi:10.1016/j.snb.2017.12.003

Cited by 72 publications

(59 citation statements)

References 50 publications

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“…The simplicity of the microfluidics with one main channel, contrast with the complexity of the dynamic square light image array to manipulate the cells. The device was tested with 8 mL of blood sample and 6.25 H209 cancer cell clusters per milliliter, with a result of excellent purification of the cell with a rate of recovery of 91.5% ± 5.6% and 70.5% ± 5.2%, respectively [62].…”

Section: Platforms Based On Circulating Tumor Cells Analysismentioning

confidence: 99%

Sensor-Integrated Microfluidic Approaches for Liquid Biopsies Applications in Early Detection of Cancer

Sierra

Marrugo-Ramírez

Rodriguez-Trujíllo

et al. 2020

Sensors

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Cancer represents one of the conditions with the most causes of death worldwide. Common methods for its diagnosis are based on tissue biopsies—the extraction of tissue from the primary tumor, which is used for its histological analysis. However, this technique represents a risk for the patient, along with being expensive and time-consuming and so it cannot be frequently used to follow the progress of the disease. Liquid biopsy is a new cancer diagnostic alternative, which allows the analysis of the molecular information of the solid tumors via a body fluid draw. This fluid-based diagnostic method displays relevant advantages, including its minimal invasiveness, lower risk, use as often as required, it can be analyzed with the use of microfluidic-based platforms with low consumption of reagent, and it does not require specialized personnel and expensive equipment for the diagnosis. In recent years, the integration of sensors in microfluidics lab-on-a-chip devices was performed for liquid biopsies applications, granting significant advantages in the separation and detection of circulating tumor nucleic acids (ctNAs), circulating tumor cells (CTCs) and exosomes. The improvements in isolation and detection technologies offer increasingly sensitive and selective equipment’s, and the integration in microfluidic devices provides a better characterization and analysis of these biomarkers. These fully integrated systems will facilitate the generation of fully automatized platforms at low-cost for compact cancer diagnosis systems at an early stage and for the prediction and prognosis of cancer treatment through the biomarkers for personalized tumor analysis.

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Section: Platforms Based On Circulating Tumor Cells Analysismentioning

confidence: 99%

Sensor-Integrated Microfluidic Approaches for Liquid Biopsies Applications in Early Detection of Cancer

Sierra

Marrugo-Ramírez

Rodriguez-Trujíllo

et al. 2020

Sensors

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“…This allows for applying up to four isolation conditions simultaneously and implementing a higher-resolution separation. As the most recent example, these authors proposed the microfluidic oDEP device for high-purity isolation of CTC clusters based on their size and preserving clusters integrity for further analysis [221]. Furthermore, oDEP was shown as a versatile tool to determine the cell electrophysiological parameters.…”

Section: Electrokinetic Cell Separationmentioning

confidence: 99%

Detection of Rare Objects by Flow Cytometry: Imaging, Cell Sorting, and Deep Learning Approaches

Voronin

Kozlova

Verkhovskii

et al. 2020

IJMS

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Flow cytometry nowadays is among the main working instruments in modern biology paving the way for clinics to provide early, quick, and reliable diagnostics of many blood-related diseases. The major problem for clinical applications is the detection of rare pathogenic objects in patient blood. These objects can be circulating tumor cells, very rare during the early stages of cancer development, various microorganisms and parasites in the blood during acute blood infections. All of these rare diagnostic objects can be detected and identified very rapidly to save a patient’s life. This review outlines the main techniques of visualization of rare objects in the blood flow, methods for extraction of such objects from the blood flow for further investigations and new approaches to identify the objects automatically with the modern deep learning methods.

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“…oDEP is a promising DEP technique developed for living/dead cell manipulation . When illumination is projected on a photoconductive surface, virtual electrodes are formed and the non‐uniform electric field is produced by direct optical images on the photoconductive surface . Amorphous silicon can be deposited on the substrate as the photoconductive layer using chemical vapor deposition with plasma enhancement.…”

Section: Configurations Of Electrodes and Dep Devicesmentioning

confidence: 99%

Recent advances in dielectrophoresis‐based cell viability assessment

et al. 2020

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Recent advances in dielectrophoresis-based cell viability assessmentDielectrophoresis (DEP) is a non-destructive, accurate, and label-free cell manipulating technique and DEP applications have been found in various fields. Assessment of cell viability is one of the important applications and many investigations have been reported. In this paper, cell polarization and its modeling, some key parameters employed for living/dead cell separation, as well as electrode configurations are reviewed. Focus is given to the latest development of DEP devices employed for the assessment of cell viability. Experimentally determined factors for separating living/dead cells, such as the conductivity of suspending medium and the frequency of applied electric field, are summarized. The future directions and potential challenges in this field are also outlined. Theory of cell DEPThe fundamentals of DEP were systematically reviewed in the past [35,36]. In this section, we only focus on the theory concerning cellular DEP. Based on the spherical particle model, single-shell model and multi-shell model of biological cells are extended. Color online: See the article online to view Figs. 1-7 in color. J. Zhang et al. Electrophoresis 2020, 41, 917-932

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Optically-induced-dielectrophoresis (ODEP)-based cell manipulation in a microfluidic system for high-purity isolation of integral circulating tumor cell (CTC) clusters based on their size characteristics

Cited by 72 publications

References 50 publications

Sensor-Integrated Microfluidic Approaches for Liquid Biopsies Applications in Early Detection of Cancer

Sensor-Integrated Microfluidic Approaches for Liquid Biopsies Applications in Early Detection of Cancer

Detection of Rare Objects by Flow Cytometry: Imaging, Cell Sorting, and Deep Learning Approaches

Recent advances in dielectrophoresis‐based cell viability assessment

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