Optical Studies of Nanodiamond-Tissue Interaction: Skin Penetration and Localization

Perevedentseva, E.; Ali, Nsrein; Karmenyan, Artashes; Skovorodkin, Ilya; Prunskaite‐Hyyryläinen, Renata; Vainio, Seppo; Cheng, Chia‐Liang

doi:10.3390/ma12223762

Cited by 15 publications

(23 citation statements)

References 98 publications

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“…Such aggregation can prevent transdermal activity, as NDs with a diameter larger than 100 nm will not penetrate to the deep skin layers. Rather, they will be localized mostly in appendages that are open to the skin, mainly hair follicles, but also in sweat gland pores and the sebaceous gland . Those appendages allow the NDs to cross the stratum corneum, which is in coherence with experiments done on other NPs .…”

Section: Introductionsupporting

confidence: 56%

Noninvasive Nanodiamond Skin Permeation Profiling Using a Phase Analysis Method: Ex Vivo Experiments

et al. 2022

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Carbon-based nanoparticles (NPs) are widely used in nanotechnology. Among them, nanodiamonds (NDs) are suitable for biotechnology and are especially interesting for skin delivery and topical treatments. However, noninvasive detection of NDs within the different skin layers or analyzing their penetration ability is complicated due to the turbid nature of the tissue. The iterative multiplane optical properties extraction (IMOPE) technique detects differences in the optical properties of the measured item by a phase-image analysis method. The phase image is reconstructed by the multiplane Gerchberg–Saxton algorithm. This technique, traditionally, detects differences in the reduced scattering coefficients. Here, however, due to the actual size of the NDs, the IMOPE technique’s detection relies on absorption analysis rather than relying on scattering events. In this paper, we use the IMOPE technique to detect the presence of the NDs within tissue-like phantoms. In addition, we perform ex vivo pigskin experiments to estimate the penetration of the NDs to the different skin layers and show that their presence reduces at deeper layers. The significance signal of the NDs within the epidermis, dermis, and fat layers gradually reduces, with t test significance values that are smaller than 10–4, 10–3, and 10–2, respectively. The IMOPE results are corroborated by TEM results and Franz-cell experiments. These results confirm that the IMOPE profiled the skin-permeation of the NDs noninvasively.

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Section: Introductionsupporting

confidence: 56%

Noninvasive Nanodiamond Skin Permeation Profiling Using a Phase Analysis Method: Ex Vivo Experiments

et al. 2022

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“…Mice of strain Crl:CD1(ICR) of 6-8 weeks age were used for the experiments, provided by Laboratory Animal Center of the Oulu University. Skin patches were obtained and prepared as described in [32].…”

Section: Skin Samples Preparation and Treatment With Aunstmentioning

confidence: 99%

Au nanostar nanoparticle as a bio-imaging agent and its detection and visualization in biosystems

Perevedentseva

Ali

Lin

et al. 2020

Biomed. Opt. Express

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In the present work, we report the imaging of Au nanostars nanoparticles (AuNSt) and their multifunctional applications in biomedical research and theranostics applications. Their optical and spectroscopic properties are considered for the multimodal imaging purpose. The AuNSt are prepared by the seed-meditated method and characterized for use as an agent for bio-imaging. To demonstrate imaging with AuNSt, penetration and localization in different biological models such as cancer cell culture (A549 lung carcinoma cell), 3D tissue model (multicellular tumor spheroid on the base of human oral squamous carcinoma cell, SAS) and murine skin tissue are studied. AuNSt were visualized using fluorescence lifetime imaging (FLIM) at two-photon excitation with a pulse duration 140 fs, repetition rate 80 MHz and 780 nm wavelength femtosecond laser. Strong emission of AuNSt at two-photon excitation in the near infrared range and fluorescence lifetime less than 0.5 ns were observed. It allows using AuNSt as a fluorescent marker at two-photon fluorescence microscopy and lifetime imaging (FLIM). It was shown that AuNSt can be observed inside a thick sample (tissue and its model). This is the first demonstration using AuNSt as an imaging agent for FLIM at two-photon excitation in biosystems. Increased scattering of near-infrared light upon excitation of AuNSt surface plasmon oscillation was also observed and rendered using a possible contrast agent for optical coherence tomography (OCT). AuNSt detection in a biological system using FLIM is compared with OCT on the model of AuNSt penetrating into animal skin. The AuNSt application for multimodal imaging is discussed.

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“…Test TiO 2 NP solutions were prepared immediately before use through dilution of the stock solutions with distilled deionized water and sonication (50 W/L, 40 kHz) for 20 min. In our studies, the particle size distributions and z-potential were estimated using the dynamic light scattering method (DLS) with a Zetasizer Nano ZS (Malvern Instruments, Malvern, UK) [36,37]. The averaged z-potential of the used TiO 2 NPs in solution was 22.08 ± 0.32 mV, with pH value around 5.35-5.45.…”

Section: Chemicals and Tio 2 Npsmentioning

confidence: 99%

Opportunistic gill infection is associated with TiO2 nanoparticle-induced mortality in zebrafish

Huang

Liu

et al. 2021

PLoS ONE

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The large amounts of engineered titanium dioxide nanoparticles (TiO2NPs) that have been manufactured have inevitably been released into the ecosystem. Reports have suggested that TiO2 is a relatively inert material that has low toxicity to animals. However, as various types of NPs increasingly accumulate in the ocean, their effects on aquatic life-forms remain unclear. In this study, a zebrafish model was used to investigate TiO2NP-induced injury and mortality. We found that the treatment dosages of TiO2NP are positively associated with increased motility of zebrafish and the bacterial counts in the water. Notably, gill but not dorsal fin and caudal fin of the zebrafish displayed considerably increased bacterial load. Metagenomic analysis further revealed that gut microflora, such as phyla Proteobacteria, Bacteroidetes, and Actinobacteria, involving more than 95% of total bacteria counts in the NP-injured zebrafish gill samples. These results collectively suggest that opportunistic bacterial infections are associated with TiO2NP-induced mortality in zebrafish. Infections secondary to TiO2NP-induced injury could be a neglected factor determining the detrimental effects of TiO2NPs on wild fish.

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Optical Studies of Nanodiamond-Tissue Interaction: Skin Penetration and Localization

Cited by 15 publications

References 98 publications

Noninvasive Nanodiamond Skin Permeation Profiling Using a Phase Analysis Method: Ex Vivo Experiments

Noninvasive Nanodiamond Skin Permeation Profiling Using a Phase Analysis Method: Ex Vivo Experiments

Au nanostar nanoparticle as a bio-imaging agent and its detection and visualization in biosystems

Opportunistic gill infection is associated with TiO2 nanoparticle-induced mortality in zebrafish

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