Opportunistic gill infection is associated with TiO2 nanoparticle-induced mortality in zebrafish

Huang, Chiao-Yi; Yu, Wei; Liu, Geng-Chia; Hung, S.; Chang, Jen-Hsiang; Chang, Jen-Che; Cheng, Chia‐Liang; Sun, Der-Shan; Lin, Ming-Der; Lin, Wen-Ying; Tzeng, Yin-Jeh; Chang, Hsin-Hou

doi:10.1371/journal.pone.0247859

Cited by 14 publications

(11 citation statements)

References 65 publications

(97 reference statements)

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“…They had a higher MR%, which could be predicted by the cytokine levels that indicated a compromised immune status. Similarly, the MR% of adult zebrafish was 100% after exposure to two different doses of TiO 2 NPs (5 and 40 mg/L) for 20 and 7 days, respectively, with blinding injury before mortality (Huang et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

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Lactobacillus plantarum ameliorates the immunological impacts of titanium dioxide nanoparticles (rutile) in Oreochromis niloticus

Sherif¹,

Khalil

Tanekhy

et al. 2022

Aquaculture Research

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Titanium dioxide (TiO2) is used in numerous applications, such as medicinal products, cosmetics and food whitening (E171). This work aimed to assess the protective role of Lactobacillus plantarum in the TiO2 nanoparticles induced toxicity in Oreochromis niloticus. A total number of 600 O. niloticus was divided into four groups G1–4; G1 was fed on the basal diet, G2 fed on L. plantarum, G3 fed on contaminated TiO2 NPs‐diet (1 mg/kg body weight), while G4 fed on contaminated TiO2 NPs‐diet then treated with L. plantarum for the same period. Fish immunity was evaluated at 10, 20 and 30 days except G4 were at 20, 40 and 60 days. The gene expression of the interleukin (IL)‐1β, IL‐6, IL‐10 and tumour necrosis factor‐α were significantly enhanced in G4 compared with G3. On days 10 and 20, the phagocytic activity was significantly increased in G4 compared with G1 and G3, while it was decreased on day 30 in G4 and G3. In addition, oxidative burst activity and serum antibacterial activity had the same trend indicating the immunosuppression effects of TiO2 NPs. The mortality rate of fish challenged against Aeromonas hydrophila was decreased in G4 (30%, 50% and 60%) compared with G3 (50%, 50% and 70%) respectively. The antioxidant enzymes catalase, glutathione peroxidase and superoxide dismutase were stimulated, indicating the generation of reactive oxygen species by TiO2 in exposed O. niloticus. It was concluded that L. plantarum could protect O. niloticus against TiO2 NPs‐induced immunosuppression by enhancing their immune response and oxidative status.

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Section: Discussionmentioning

confidence: 99%

“…of TiO 2 NPs (5 and 40 mg/L) for 20 and 7 days, respectively, with blinding injury before mortality (Huang et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

Lactobacillus plantarum ameliorates the immunological impacts of titanium dioxide nanoparticles (rutile) in Oreochromis niloticus

Sherif¹,

Khalil

Tanekhy

et al. 2022

Aquaculture Research

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“…Higher microbiome diversity could therefore reflect infection, to which reduced activity level is a common behavioural response [72]. Indeed, Sphingobacteriales.f_env.OPS bacteria is an opportunistic pathogen of zebrafish gills [73]. Alternatively, these microbes could be affecting the host through many different physiological pathways such as hormone production, olfactory modulation and inflammation, which could influence the fish's activity level without active disease [74].…”

Section: Discussionmentioning

confidence: 99%

Skin bacterial microbiome diversity predicts lower activity levels in female, but not male, guppies,Poecilia reticulata

2022

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While the link between the gut microbiome and host behaviour is well established, how the microbiomes of other organs correlate with behaviour remains unclear. Additionally, behaviour–microbiome correlations are likely sex-specific because of sex differences in behaviour and physiology, but this is rarely tested. Here, we tested whether the skin microbiome of the Trinidadian guppy, Poecilia reticulata , predicts fish activity level and shoaling tendency in a sex-specific manner. High-throughput sequencing revealed that the bacterial community richness on the skin (Faith's phylogenetic diversity) was correlated with both behaviours differently between males and females. Females with richer skin-associated bacterial communities spent less time actively swimming. Activity level was significantly correlated with community membership (unweighted UniFrac), with the relative abundances of 16 bacterial taxa significantly negatively correlated with activity level. We found no association between skin microbiome and behaviours among male fish. This sex-specific relationship between the skin microbiome and host behaviour may indicate sex-specific physiological interactions with the skin microbiome. More broadly, sex specificity in host–microbiome interactions could give insight into the forces shaping the microbiome and its role in the evolutionary ecology of the host.

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“…USA) ( 56 , 57 ), and red fluorescent NDs (50 nm; brFND-50, nitrogen-vacancy NV centers per particle > 100, FND Biotech, Taipei, Taiwan) ( 58 , 59 ), the nanoparticles (NPs) were dispersed in distilled deionized water under sonication (80 W/L, 46 kHz) for 20 min. Test NP solutions were prepared immediately before use by dilution of the stock solutions with distilled deionized water and sonication (80 W/L, 46 kHz) for 20 min ( 60 ).…”

Section: Methodsmentioning

confidence: 99%

Nanodiamond-Induced Thrombocytopenia in Mice Involve P-Selectin-Dependent Nlrp3 Inflammasome-Mediated Platelet Aggregation, Pyroptosis and Apoptosis

Hung

Lien

et al. 2022

Front. Immunol.

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Nanodiamond (ND) has been developed as a carrier to conduct various in vivo diagnostic and therapeutic uses. Safety is one of the major considerations, while the hemocompatibility of ND is not clearly addressed. Here we found that, compared to the other sizes of ND with relatively inert properties, treatments of 50 nm ND induced stronger platelet aggregation, platelet pyroptosis, apoptosis and thrombocytopenia in mice. Blockage treatments of soluble P-selectin, reactive oxygen species (ROS), and Nlrp3 inflammasome inhibitors markedly suppressed such adverse effects, suggesting ND-induced platelet activation and pyroptosis involves surface P-selectin-mediated enhancement of mitochondrial superoxide levels and Nlrp3 inflammasome activation. In addition, challenges of NDs induced less platelet pyroptosis and displayed less thrombocytopenia in P-selectin (Selp-/-), Nlrp3 (Nlrp3-/-) and caspase-1 (Casp1-/-) mutants, as compared to the wild type mice. Blockers of P-selectin, ROS, and Nlrp3 inflammasome pathways could be considered as antidotes for ND induced platelet activation and thrombocytopenia.

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Opportunistic gill infection is associated with TiO2 nanoparticle-induced mortality in zebrafish

Cited by 14 publications

References 65 publications

Lactobacillus plantarum ameliorates the immunological impacts of titanium dioxide nanoparticles (rutile) in Oreochromis niloticus

Lactobacillus plantarum ameliorates the immunological impacts of titanium dioxide nanoparticles (rutile) in Oreochromis niloticus

Skin bacterial microbiome diversity predicts lower activity levels in female, but not male, guppies,Poecilia reticulata

Nanodiamond-Induced Thrombocytopenia in Mice Involve P-Selectin-Dependent Nlrp3 Inflammasome-Mediated Platelet Aggregation, Pyroptosis and Apoptosis

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