Skin bacterial microbiome diversity predicts lower activity levels in female, but not male, guppies,<i>Poecilia reticulata</i>

Kramp, Rachael D.; Kohl, Kevin D.; Stephenson, Jessica F.

doi:10.1098/rsbl.2022.0167

Cited by 3 publications

(7 citation statements)

References 79 publications

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“…Alpha diversity was negatively correlated with both behaviours; sh with higher Chao1 and Faith_pd diversities displayed lower levels of activity and inspection. These results mirror the decline in activity with increasing Faith_pd skin diversity previously observed in female guppies [14]. Dysbiosis due to infection, hormonal production or olfactory regulation were proposed to explain the correlation in the case of the guppies, but whether there is a causal relationship and its possible direction was unclear [14].…”

Section: Discussionsupporting

confidence: 61%

“…Germ-free zebra sh and larvae supplemented with the probiotic Lactobacillus plantarum display less anxiety-related behaviours compared to controls raised under standard conditions, supporting the suggestion that the microbiome plays a role in regulating the stress response in this sh [13]. Skin microbiota also seems to in uence activity levels in sh in a sex-speci c manner, as a recent study in guppies indicated [14]. The association between microbiome and behaviour can be particularly important given the negative effects of stress observed in the sh skin and gut microbiomes [15].…”

Section: Introductionsupporting

confidence: 53%

“…Yet, the changes observed in behaviour, physiology and gut microbiota composition associated with rank change in African cichlids (Astatotilapia burtoni) for example, indicate that the microbiome-gut-brain axis is likely to be important for other sh species as well [60]. The disruption of the sh skin microbiome has also been related to stress in salmonids [15,61] and to sex-speci c activity in guppies [14], although the potential route of interaction is less clear than in the case of the gut.…”

Section: Discussionmentioning

confidence: 99%

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Rearing environment influences the interaction between fish gut microbiota and behaviour

Anka,

Webster,

McLaughlin

et al. 2024

Preprint

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Background The gut microbiota influences human and animal cognition and behaviour through its effects on the endocrine and immune systems, but the relationship between host genetics, environmental factors and the microbiota, and their joint effect on the host cognitive behaviour has not been determined. The microbiome-behaviour relationship may be especially relevant for fish, due to their diverse evolutionary history and potential applications for farming and conservation. Yet, there is limited research of the interaction between gut microbiome and behaviour in fish, apart from using zebrafish as a model for human applications. We manipulated the rearing environment and diet of fish from two highly inbred strains of the self-fertilising mangrove killifish (Kryptolebias marmoratus) and assessed their effects on the gut microbiome (based on 16S rRNA sequencing) and the potential interactions with behaviour (based on a novel object test). Results We found that the microbiome composition and alpha diversity were significantly influenced by strain, hatching mode and diet, but not by environmental enrichment. We also identified a strong association between fish gut microbiota (community composition and alpha diversity measured as Chao1 and Faith phylogenetic diversity) and behaviour (activity level and inspections of a novel object), but no significant influence of strain, diet, environment or hatching mode on behaviour. The particular taxa influenced by host genetics and environmental factors were not the same as those associated with the behaviour, but in both cases were dominated by Bacteroidales, potentially related to the production of SCFAs and neurodevelopment. Conclusion We suggest thatthe observed association between microbiome and fish behaviour could be an indirect effect of the modulation of the ecological conditions of the gut microbiota by the host genetics and rearing conditions (hatching and diet), which could be affecting the production of microbial metabolites that interact with the fish physiology.

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Section: Discussionsupporting

confidence: 61%

Section: Introductionsupporting

confidence: 53%

Section: Discussionmentioning

confidence: 99%

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Rearing environment influences the interaction between fish gut microbiota and behaviour

Anka,

Webster,

McLaughlin

et al. 2024

Preprint

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“…However, studies on several other vertebrates found no evidence for biological sex to be a significant variable shaping skin microbial communities 6 – 8 . Recent studies have explored the relationship between fish biological sex and skin microbiome 9 – 11 . For instance, female guppies found to have a higher abundance of certain bacterial taxa, such as Flavobacterium, compared to males 9 .…”

Section: Introductionmentioning

confidence: 99%

“…Recent studies have explored the relationship between fish biological sex and skin microbiome 9 – 11 . For instance, female guppies found to have a higher abundance of certain bacterial taxa, such as Flavobacterium, compared to males 9 . These differences are thought to be driven by differences in hormonal profiles and immune function between the sexes.…”

Section: Introductionmentioning

confidence: 99%

Sex-dependent effects of mechanical delousing on the skin microbiome of broodstock Atlantic salmon (Salmo salar L.)

Casadei,

Mani,

Cisco

et al. 2023

Sci Rep

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Delousing strategies, including mechanical delousing, are typically used to treat Atlantic salmon (Salmo salar) sea lice infestations. In this study, we evaluate the impact of mechanical delousing (Hydrolicer) on the skin bacterial microbiome of broodstock female and male Atlantic salmon. 16S rDNA sequencing of salmon skin microbial communities was performed immediately before delousing, right after delousing and 2 and 13 days post-delousing (dpd). The skin bacterial community of female salmon was more diverse than that of males at the start of the experiment. Overall, hydrolycer caused losses in alpha diversity in females and increases in alpha diversity in males. Hydrolicer also caused rapid shifts in the skin microbial community composition immediately after delicing in a sex-specific manner. There was a decrease in abundance of Proteobacteria and Bacteriodetes in both female and male salmon, whereas Firmicutes and Tenericutes abundances increased. Interestingly, the female community recovered faster, while the male community remained dysbiotic 13 dpd due to expansions in Bacteroidetes (Pseudomonadaceae) and Firmicutes. Our data suggest that female broodstock are more resilient to Hydrolicer treatment due to their more diverse skin microbiota community, and that sex influences the skin microbial community and therefore host health outcomes during common farming manipulations.

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Dietary tannins alter growth, behavior, and the gut microbiome of larval amphibians

2023

Self Cite

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Research has shown that leached plant toxins negatively impact the growth and development of larval amphibians. However, tadpoles may encounter these same toxins in food material, and differential exposure routes and distribution of toxic chemicals can yield variable downstream effects on animals. To date, most research understanding the interactions between dietary plant toxins and herbivores has been conducted in terrestrial systems. Despite the abundance of plant toxins in food and water sources, the effects of dietary plant toxins on larval amphibians have not been studied, and tannins could negatively affect these species. Here, green frog tadpoles (Lithobates clamitans) were fed diets with or without 2% tannic acid to test how their growth, development, behavior, and gut microbiome respond to dietary tannins. At the end of the trial, we conducted a behavioral assay to measure tadpole activity and boldness and inventoried the gut microbiome using 16S rRNA sequencing. Dietary tannins significantly decreased body mass by 66% and length by 28%, without influencing tadpole developmental stage. We found significant differences in exploratory behavior and boldness during the first minute of our behavioral assay, demonstrating that tannins have the potential to influence behavior during novel or stressful events. Finally, tannins significantly sculpted the gut microbiome, with an increase in the measurement of Shannon entropy. We observed 7 microbial phyla and 153 microbial genera that exhibited significantly differential abundances differences between control and tannic acid‐fed tadpoles. Collectively, our results demonstrate that dietary tannins have the potential to alter amphibian growth, behavior, and microbiome.

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Skin bacterial microbiome diversity predicts lower activity levels in female, but not male, guppies,Poecilia reticulata

Cited by 3 publications

References 79 publications

Rearing environment influences the interaction between fish gut microbiota and behaviour

Rearing environment influences the interaction between fish gut microbiota and behaviour

Sex-dependent effects of mechanical delousing on the skin microbiome of broodstock Atlantic salmon (Salmo salar L.)

Dietary tannins alter growth, behavior, and the gut microbiome of larval amphibians

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