Optical sectioning microscopes with no moving parts using a micro-stripe array light emitting diode

Poher, V.; Zhang, H. X.; Kennedy, Gordon T.; Griffin, C.; Oddos, Stéphane; Gu, Erdan; Elson, Daniel S.; Girkin, John M.; French, Paul M. W.; Dawson, Martin D.; Neil, Mark A. A.

doi:10.1364/oe.15.011196

Cited by 52 publications

(36 citation statements)

References 11 publications

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“…Various methods of cosine fringe projection have been demonstrated in SIM systems, including mechanically moving grating, 5,6 two beam interference illumination, 7 micro-stripe array light-emitting diodes (LED), 8 and liquid crystal spatial light modulators. 9 Despite these efforts, an illumination pattern transition time of several tens of milliseconds still limits the net frame rate of SIM to a few hertz.…”

mentioning

confidence: 99%

Fast optical sectioning obtained by structured illumination microscopy using a digital mirror device

Jiang

et al. 2013

J. Biomed. Opt

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confidence: 99%

Fast optical sectioning obtained by structured illumination microscopy using a digital mirror device

Jiang

et al. 2013

J. Biomed. Opt

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“…The incoherent non-super-resolution SIM systems, which are the focus of our analysis, share all the common operations: projecting a patterned illumination on the sample, phase-shifting the pattern in accurate steps over the specimen, determining the contrast values for all pixels, shifting the specimen in steps of z s through focus, extracting the focal depth response (FDR) for all pixels and determining the focal position of each pixel to generate the topography of the specimen. The different existing SIM systems differ in their shapes of the patterns (sinusoidal, [4,[9][10][11][12][13][14] rectangular [15][16][17][18][19][20] ), their algorithms, their pattern generations (grating, [1,4,9,[20][21][22][23][24][25] digital light modulator (DMD), [10,12,16,26] spatial light modulator (SLM), [27][28][29][30] light emitting diode (LED) array, [19] interference [10] ) and their manners of shifting the pattern over the sample (high precision linear actuator, [1,4,10,15,20] rotational actuator, [24,25] micro mirrors, [11,12,16,…”

Section: Introductionmentioning

confidence: 99%

“…Although SIM systems with no moving parts exist, [17,19,[27][28][29][30] the majority of SIM systems uses a physical grating which is shifted by an actuator. It is the great benefit of the grating compared to DMD, SLM and other systems that the simple Center contrast value on the FDR C À1 , C þ1…”

Section: Introductionmentioning

confidence: 99%

Fault Tolerant Algorithm for Structured Illumination Microscopy with Incoherent Light

Wang

Heidingsfelder

Gao

et al. 2015

International Journal of Optomechatronics

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In this contribution we present a new algorithm for structured illumination microscopy with incoherent light. Existing algorithms for determining the contrast values of the focal depth response require a high accurate phase shift of the fringe pattern illumination. The presented algorithm, which is robust against inaccurate phase shift of the fringe pattern, reduces significantly the requirements for the phase shift and consequently the costs of the microscope. The new algorithm was tested by a preliminary experiment, whereby the grating was shifted by an elastic guided micro-motion mechanism employing a low-cost stepper motor replacing the conventional expensive piezo drive. The determined focal depth response is very smooth and corresponds very well to the theoretical focal depth response.

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“…Appropriate illumination of the specimen is an important factor in achieving high-resolution and high-quality images in microscopy and critical photomicrography. With the maturation of LED technology, the use of LEDs as the light source for optical microscopy can bring certain cost-and usage-advantages [3,7,8]. In this Letter, we demonstrate a simple and cost-effective microscopy illumination scheme by replacing the optical condenser with a programmable LED array.…”

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confidence: 99%

“…It can provide specimen illumination that is uniformly bright and free from glare [1]. More advanced illumination schemes have also been reported in recent years, including structured illumination [2,3], light sheet illumination [4], focusgrid illumination [5], and nondiffracted Bessel beam illumination [6].…”

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Microscopy refocusing and dark-field imaging by using a simple LED array

2011

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The condenser is one of the main components in most transmitted light compound microscopes. In this Letter, we show that such a condenser can be replaced by a programmable LED array to achieve greater imaging flexibility and functionality. Without mechanically scanning the sample or changing the microscope setup, the proposed approach can be used for dark-field imaging, bright-field imaging, microscopy sectioning, and digital refocusing. Images of a starfish embryo were acquired by using such an approach for demonstration. © 2011 Optical Society of America OCIS codes: 110.0180, 110.2945, 110.3010, 180.6900. Appropriate illumination of the specimen is an important factor in achieving high-resolution and high-quality images in microscopy and critical photomicrography. With the maturation of LED technology, the use of LEDs as the light source for optical microscopy can bring certain cost-and usage-advantages [3,7,8]. In this Letter, we demonstrate a simple and cost-effective microscopy illumination scheme by replacing the optical condenser with a programmable LED array. The proposed illumination scheme has several advantages. 1) A conventional bright-field image can be acquired by digitally matching the illumination numerical aperture (NA) to the collection NA (i.e. NA of objective lens). 2) A dark-field image of the specimen can be acquired by simply turning on the LEDs at the edge of the array, where the illumination NA is beyond the collection NA. 3) We can sequentially turn on each individual LED and capture a sequence of specimen images. These images contain the information for different view angles and therefore we can postprocess them to digitally refocus the specimen into different depths. 4) The aforementioned imaging schemes can be accomplished simultaneously by a single LED scan process. We can select the images corresponding to LEDs within the collection NA to form a bright-field image. On the other hand, the images corresponding to LEDs beyond the collection NA can be used for dark-field imaging. Furthermore, we can realign all the images (with different view angles) to digitally refocus the specimen into different depths. 5) No mechanical moving parts are involved in the proposed scheme. The scanning rate of the LEDs can easily operate in the kHz domain. The limiting factor in our prototype is the capturing frame rate/data transfer rate of the camera. Because of the rapid development of the semiconductor industry, we believe that such a data transfer rate will not be a significant bottleneck for the proposed illumination scheme in the near future. 6) The proposed scheme is cost-effective and compatible with most modern laboratory microscopes.This Letter is structured as follows. We will first describe the experimental setup of our proposed illumination scheme. Next, we will report on our results of bright-field and dark-field imaging capabilities. We will then report on our demonstration of digital refocusing of the specimen into different depths. Finally, we will draw our conclusion at the end of...

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Optical sectioning microscopes with no moving parts using a micro-stripe array light emitting diode

Cited by 52 publications

References 11 publications

Fast optical sectioning obtained by structured illumination microscopy using a digital mirror device

Fast optical sectioning obtained by structured illumination microscopy using a digital mirror device

Fault Tolerant Algorithm for Structured Illumination Microscopy with Incoherent Light

Microscopy refocusing and dark-field imaging by using a simple LED array

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