Optical-resolution photoacoustic endomicroscopy in vivo

Yang, Joon-Mo; Li, Chiye; Chen, Ruimin; Rao, Bin; Yao, Junjie; Yeh, Cheng-Hung; Danielli, Amos; Maslov, Konstantin; Zhou, Qifa; Shung, K. Kirk; Wang, Lihong V.

doi:10.1364/boe.6.000918

Cited by 80 publications

(85 citation statements)

References 48 publications

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“…In addition to ongoing challenge of finding an acoustic matching medium that does not need replacement, as we discussed in our recent report on this OR-PAEM work [59], another urgent task is to increase the sensitivity of the imaging probe. In this study, we achieved a 29 dB SNR for the 3-µm diameter microsphere illuminated by a 500-nJ pulse energy in a 9.2 µm beam diameter, which yielded a surface fluence of ~44 mJ/cm 2 , about two times greater than the ANSI safety limit (20 mJ/cm 2 ) for allowable skin laser fluence [62].…”

Section: Discussionmentioning

confidence: 99%

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Label-free optical-resolution photoacoustic endomicroscopy in vivo

Yang

Chen

et al. 2015

SPIE Proceedings

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Intravital microscopy techniques have become increasingly important in biomedical research because they can provide unique microscopic views of various biological or disease developmental processes in situ. Here we present an opticalresolution photoacoustic endomicroscopy (OR-PAEM) system that visualizes internal organs with a much finer resolution than conventional acoustic-resolution photoacoustic endoscopy systems. By combining gradient index (GRIN) lens-based optical focusing and ultrasonic ring transducer-based acoustic focusing, we achieved a transverse resolution as fine as ~10 μm at an optical working distance of 6.5 mm. The OR-PAEM system's high-resolution intravital imaging capability is demonstrated through animal experiments.

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Section: Discussionmentioning

confidence: 99%

“…More detailed information on the imaging probe and peripheral systems is available in our recent report [59].…”

Section: Design and Construction Of The Or-paem Probe And Peripheral mentioning

confidence: 99%

Label-free optical-resolution photoacoustic endomicroscopy in vivo

Yang

Chen

et al. 2015

SPIE Proceedings

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“…It is an excellent avenue for interrogating angiogenesis and blood content in highly aggressive tumors, and shows great potential for molecular imaging [5]. PAI and Photoacoustic Tomography (PAT) have been developing for many years in both clinical and preclinical regimes with applications in skin [6] and breast cancer [7,8], lymph node mapping [9][10][11], and endoscopy [12,13]. These technique relies on absorption of light to create contrast, a phenomenon that is governed by both tissue optical properties and light fluence.…”

Section: Introductionmentioning

confidence: 99%

Quantitative photoacoustic image reconstruction improves accuracy in deep tissue structures

Mastanduno¹,

Gambhir²

2016

Biomed. Opt. Express

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Photoacoustic imaging (PAI) is emerging as a potentially powerful imaging tool with multiple applications. Image reconstruction for PAI has been relatively limited because of limited or no modeling of light delivery to deep tissues. This work demonstrates a numerical approach to quantitative photoacoustic image reconstruction that minimizes depth and spectrally derived artifacts. We present the first time-domain quantitative photoacoustic image reconstruction algorithm that models optical sources through acoustic data to create quantitative images of absorption coefficients. We demonstrate quantitative accuracy of less than 5% error in large 3 cm diameter 2D geometries with multiple targets and within 22% error in the largest size quantitative photoacoustic studies to date (6cm diameter). We extend the algorithm to spectral data, reconstructing 6 varying chromophores to within 17% of the true values. This quantitiative PA tomography method was able to improve considerably on filtered-back projection from the standpoint of image quality, absolute, and relative quantification in all our simulation geometries. We characterize the effects of time step size, initial guess, and source configuration on final accuracy. This work could help to generate accurate quantitative images from both endogenous absorbers and exogenous photoacoustic dyes in both preclinical and clinical work, thereby increasing the information content obtained especially from deep-tissue photoacoustic imaging studies. Hahn, and A. G. Yodh, "In vivo reflectance measurement of optical properties, blood oxygenation and motexafin lutetium uptake in canine large bowels, kidneys and prostates," Phys. Med. Biol. 47(6), 857-873 (2002)

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“…Fully encapsulated AR-PAM endoscopes have been demonstrated by Yang et al [17,18] using a single mode fiber and an actuation system for the beam steering. Recently OR-PAM images have been acquired by the same group using a device with an outer diameter of 3.4 mm and a lateral resolution of 9.2 μm [19]. OR-PAM by using a MMF has been demonstrated by Papadopoulos et al in [20].…”

Section: Introductionmentioning

confidence: 99%

Towards new applications using capillary waveguides

Stasio¹,

Shibukawa²,

Papadopoulos³

et al. 2015

Biomed. Opt. Express

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Abstract:In this paper we demonstrate the enhancement of the sensing capabilities of glass capillaries. We exploit their properties as optical and acoustic waveguides to transform them potentially into high resolution minimally invasive endoscopic devices. We show two possible applications of silica capillary waveguides demonstrating fluorescence and opticalresolution photoacoustic imaging using a single 330 μm-thick silica capillary. A nanosecond pulsed laser is focused and scanned in front of a capillary by digital phase conjugation through the silica annular ring of the capillary, used as an optical waveguide. We demonstrate optical-resolution photoacoustic images of a 30 μm-thick nylon thread using the water-filled core of the same capillary as an acoustic waveguide, resulting in a fully passive endoscopic device. Moreover, fluorescence images of 1.5 μm beads are obtained collecting the fluorescence signal through the optical waveguide. This kind of silica-capillary waveguide together with wavefront shaping techniques such as digital phase conjugation, paves the way to minimally invasive multi-modal endoscopy.

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Optical-resolution photoacoustic endomicroscopy in vivo

Cited by 80 publications

References 48 publications

Label-free optical-resolution photoacoustic endomicroscopy in vivo

Label-free optical-resolution photoacoustic endomicroscopy in vivo

Quantitative photoacoustic image reconstruction improves accuracy in deep tissue structures

Towards new applications using capillary waveguides

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