Optical microscopy of ultrathin tissue sections

Moore, Richard D.; Mumaw, V. R.; Schoenberg, Melvin D.

doi:10.1016/s0022-5320(60)90047-2

Cited by 53 publications

(17 citation statements)

References 4 publications

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“…Sections were obtained with a Reichert Ultracut S ultratome (Leica, Wien, Austria). Semi-thin sections (0.75 μm in thickness) were stained by crystal violet and basic fuchsin [29] and observed under the light microscope Nikon Eclipse Ni (Nikon, Tokyo, Japan). Data were recorded with a DS-5 M-L1 digital camera system (Nikon).…”

Section: Methodsmentioning

confidence: 99%

AIF-1 and RNASET2 Play Complementary Roles in the Innate Immune Response of Medicinal Leech

et al. 2018

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Recent studies demonstrated that allograft inflammatory factor-1 (AIF-1) and RNASET2 act as chemoattractants for macrophages and modulate the inflammatory processes in both vertebrates and invertebrates. The expression of these proteins significantly increases after bacterial infection; however, the mechanisms by which they regulate the innate immune response are still poorly defined. Here, we evaluate the effect of bacterial lipopolysaccharide injection on the expression pattern of these genes and the interrelation between them during innate immune response in the medicinal leech, an invertebrate model with a simple anatomy and a marked similarity with vertebrates in inflammatory processes. Collectively, prokaryotic-eukaryotic co-cultures and in vivo infection assays suggest that RNASET2 and AIF-1 play a crucial role in orchestrating a functional cross-talk between granulocytes and macrophages in leeches, resulting in the activation of an effective response against pathogen infection. RNASET2, firstly released by granulocytes, likely plays an early antibacterial role. Subsequently, AIF-1⁺ RNASET2-recruited macrophages further recruit other macrophages to potentiate the antibacterial inflammatory response. These experimental data are in keeping with the notion of RNASET2 acting as an alarmin-like molecule whose role is to locally transmit a “danger” signal (such as a bacterial infection) to the innate immune system in order to trigger an appropriate host response.

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Section: Methodsmentioning

confidence: 99%

AIF-1 and RNASET2 Play Complementary Roles in the Innate Immune Response of Medicinal Leech

et al. 2018

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“…Sections were obtained with a Reichert Ultracut S ultratome (Leica, Wien, Austria). Semi-thin sections (0.75 μm in thickness) were stained by conventional methods (crystal violet and basic fuchsin, according to Moore et al 1960) and subsequently observed under a light microscope (Nikon Eclipse Ni, Nikon, Tokyo, Japan). Data were recorded with a DS-5 M-L1 digital camera system (Nikon).…”

Section: Animals and Treatmentsmentioning

confidence: 99%

Homolog of allograft inflammatory factor-1 induces macrophage migration during innate immune response in leech

et al. 2014

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Allograft inflammatory factor-1 (AIF-1) is a 17-kDa cytokine-inducible calcium-binding protein that, in vertebrates, plays an important role in the allograft immune response. Its expression is mostly limited to the monocyte/macrophage lineage. Until recently, AIF-1 was assumed to be a novel molecule involved in inflammatory responses. To clarify this aspect, we have investigated the expression of AIF-1 after bacterial challenge and its potential role in regulating the innate immune response in an invertebrate model, the medicinal leech (Hirudo medicinalis). Analysis of an expressed sequence tag library from the central nervous system of Hirudo revealed the presence of the gene Hmaif-1/alias Hmiba1, showing high homology with vertebrate aif-1. Immunohistochemistry with an anti-HmAIF-1 polyclonal antibody revealed the constitutive presence of this protein in spread CD68(+) macrophage-like cells. A few hours after pathogen (bacterial) injection into the body wall, the amount of these immunopositive cells co-expressing HmAIF-1 and the common leucocyte marker CD45 increased at the injected site. Moreover, the recombinant protein HmAIF-1 induced massive angiogenesis and was a potent chemoattractant for macrophages. Following rHmAIF-1 stimulation, macrophage-like cells co-expressed the macrophage marker CD68 and the surface glycoprotein CD45, which, in vertebrates, seems to have a role in the integrin-mediated adhesion of macrophages and in the regulation of the functional responsiveness of cells to chemoattractants. CD45 is therefore probably involved in leech macrophage-like cell activation and migration towards an inflammation site. We have also examined its potential effect on HmAIF-1-induced signalling.

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“…Cross sections (1 pm thick) of worms obtained with an Ultrotome V LKB were stained with cristal violet and basic fuchsin, according to Moore et al, (1960).…”

Section: Semi-thin Sectionsmentioning

confidence: 99%

Ultrastructural and histochemical comparison in two haplotaxids

1994

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Pelodrilus leruthi Hrabe, 1958 and Haplotaxis gordioides Hartman, 1821 have been investigated from a histochemical point of view. A different distribution and number of fast and slow fibres has been detected in the two species. Pelodrilus leruthi specimens living in different caves, show some differences in the body wall muscle thickness and in the structure of the ventral nerve cord. A correlation with their different life styles is proposed.

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Optical microscopy of ultrathin tissue sections

Cited by 53 publications

References 4 publications

AIF-1 and RNASET2 Play Complementary Roles in the Innate Immune Response of Medicinal Leech

AIF-1 and RNASET2 Play Complementary Roles in the Innate Immune Response of Medicinal Leech

Homolog of allograft inflammatory factor-1 induces macrophage migration during innate immune response in leech

Ultrastructural and histochemical comparison in two haplotaxids

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