Optical Investigation of Individual Red Blood Cells for Determining Cell Count and Cellular Hemoglobin Concentration in a Microfluidic Channel

Reichenwallner, Ann-Kathrin; Vurmaz, Esma; Battis, Kristina; Handl, Laura; Üstün, Helin; Mach, Tivadar; Hörnig, Gabriele; Lipfert, Jan; Richter, Lukas

doi:10.3390/mi12040358

Cited by 5 publications

(4 citation statements)

References 42 publications

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“…Determination of peripheral red blood parameters. Erythrocytes were counted using the Goryaev counting grid [19], and the hemoglobin content was determined with a Sally hemoglobinometer. The relative percentage of hemoglobin was divided by the double number of the rst two numbers of erythrocytes to obtain a color index.…”

Section: Methodsmentioning

confidence: 99%

Impact of Immobilization Stress on Erythropoiesis, Alterations in the Antioxidant System, and Potential Correction with Stevia rebaudiana Bertoni in Rabbit

Karapetyan,

Barseghyan,

Karapetyan

et al. 2024

Preprint

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This study investigates the impact of immobilization stress as a risk factor for pathological processes, with a focus on erythropoiesis and oxidative stress. Additionally, the potential therapeutic effects of Stevia rebaudiana Bertoni in mitigating these stress-induced alterations are explored. Immobilization stress was induced for 30 days, and its effects on erythropoiesis and antioxidant systems were assessed. Stevia rebaudiana Bertoni was administered to evaluate its potential for preventing and correcting the observed negative processes. Prolonged immobilization stress led to disruptions in erythropoiesis, manifested by a delay in proliferative and maturation processes and a moderate decrease in erythrocyte equilibrium. Concurrently, increased stress duration stimulated lipid peroxidation processes and inhibited antioxidant enzyme activity. The use of Stevia rebaudiana Bertoni demonstrated significant positive effects on lipid peroxidation processes and restored the activity of antioxidant enzymes, particularly peroxidase and catalase. Immobilization stress adversely affects erythropoiesis and oxidative balance, contributing to pathological processes. Stevia rebaudiana Bertoni emerges as a promising intervention, displaying the potential to attenuate the negative effects of immobilization stress through its positive influence on erythropoiesis and antioxidant mechanisms.

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Section: Methodsmentioning

confidence: 99%

Impact of Immobilization Stress on Erythropoiesis, Alterations in the Antioxidant System, and Potential Correction with Stevia rebaudiana Bertoni in Rabbit

Karapetyan,

Barseghyan,

Karapetyan

et al. 2024

Preprint

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show abstract

“…The rise of microfluidics in the last two decades has enabled the increase in experimental options to study RBCs' properties and their effect on blood flow [89,[100][101][102]. The easiness of replicating small structures in microfluidics allowed the development of various designs and structures to observe and analyze the deformation of RBCs [103][104][105]. Typical microfluidics approaches consider a forced, or gradual, constriction of RBCs, as they circulate through very narrow slits.…”

Section: Hemodynamics and Hemorheology For A Single Cellmentioning

confidence: 99%

Microfluidics Approach to the Mechanical Properties of Red Blood Cell Membrane and Their Effect on Blood Rheology

et al. 2022

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In this article, we describe the general features of red blood cell membranes and their effect on blood flow and blood rheology. We first present a basic description of membranes and move forward to red blood cell membranes’ characteristics and modeling. We later review the specific properties of red blood cells, presenting recent numerical and experimental microfluidics studies that elucidate the effect of the elastic properties of the red blood cell membrane on blood flow and hemorheology. Finally, we describe specific hemorheological pathologies directly related to the mechanical properties of red blood cells and their effect on microcirculation, reviewing microfluidic applications for the diagnosis and treatment of these diseases.

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“…Whole blood consists of plasma (55% by volume) and formed elements (45% by volume), 99% of which are erythrocytes (hemoglobin-containing elements) and 1% are leukocytes and platelets [23,24]. Under normal physiological conditions (erythrocytes are not deformable), the optics of whole blood, as a highly concentrated turbid medium, is determined by the properties of erythrocytes and plasma.…”

Section: Theoretical Aspectsmentioning

confidence: 99%

Analysis of Polarization Images in the Microphysical Blood Parameters Research for the Hematocrit Diagnostics

et al. 2022

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The development of non-invasive optoelectronic technologies for human blood monitoring is one of the important research areas for medicine. A critical analysis of optoelectronic methods of blood research and the micromechanical systems based on them is carried out in this article. A design realization of a polarizing portable system for non-invasive monitoring of hematocrit as one of the basic homeostatic constants of the human body containing information about the microphysical parameters of blood cells has been substantiated. A physical model of polarized radiation conversion in a video information system of laser sensing of a biological research object has been formed. Visual and quantitative differences in the spatial distribution of polarization parameters of the scattered radiation for the states of the body with different hematocrit levels have been revealed. A scheme of a multichannel imaging portable system, based on a smartphone using miniature optical and microelectronic components of information conversion for non-invasive monitoring of microphysical blood parameters, has been created. The system implements the principle of polarimetric blood photometry and a multiparametric analysis of the polarization properties of the laser radiation scattered by blood. The developed portable optoelectronic system, based on a smartphone, can be used for rapid blood diagnostics in disaster medicine and the presence of clinical contraindications to the formation of invasive tests. The proposed polarization-based approach is a promising automated alternative to traditional devices and systems for the research of microphysical blood parameters.

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Optical Investigation of Individual Red Blood Cells for Determining Cell Count and Cellular Hemoglobin Concentration in a Microfluidic Channel

Cited by 5 publications

References 42 publications

Impact of Immobilization Stress on Erythropoiesis, Alterations in the Antioxidant System, and Potential Correction with Stevia rebaudiana Bertoni in Rabbit

Impact of Immobilization Stress on Erythropoiesis, Alterations in the Antioxidant System, and Potential Correction with Stevia rebaudiana Bertoni in Rabbit

Microfluidics Approach to the Mechanical Properties of Red Blood Cell Membrane and Their Effect on Blood Rheology

Analysis of Polarization Images in the Microphysical Blood Parameters Research for the Hematocrit Diagnostics

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